Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_22955 and AO356_22960 are separated by 66 nucleotides AO356_22960 and AO356_22965 are separated by 109 nucleotides AO356_22965 and AO356_22970 are separated by 83 nucleotides
AO356_22955: AO356_22955 - ArsR family transcriptional regulator, at 5,124,864 to 5,125,631
_22955
AO356_22960: AO356_22960 - AraC family transcriptional regulator, at 5,125,698 to 5,126,489
_22960
AO356_22965: AO356_22965 - hypothetical protein, at 5,126,599 to 5,127,528
_22965
AO356_22970: AO356_22970 - Branched-chain amino acid aminotransferase (EC 2.6.1.42) <small>(from data)</small>, at 5,127,612 to 5,128,631
_22970
Position (kb) 5126
5127
5128 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5125.706 kb on + strand at 5125.706 kb on + strand at 5125.707 kb on - strand at 5125.707 kb on - strand at 5125.707 kb on - strand at 5125.995 kb on + strand, within AO356_22960 at 5126.032 kb on + strand, within AO356_22960 at 5126.032 kb on + strand, within AO356_22960 at 5126.033 kb on - strand, within AO356_22960 at 5126.218 kb on + strand, within AO356_22960 at 5126.219 kb on - strand, within AO356_22960 at 5126.219 kb on - strand, within AO356_22960 at 5126.308 kb on + strand, within AO356_22960 at 5126.309 kb on - strand, within AO356_22960 at 5126.309 kb on - strand, within AO356_22960 at 5126.309 kb on - strand, within AO356_22960 at 5126.347 kb on + strand, within AO356_22960 at 5126.348 kb on - strand, within AO356_22960 at 5126.348 kb on - strand, within AO356_22960 at 5126.389 kb on + strand, within AO356_22960 at 5126.536 kb on + strand at 5126.537 kb on - strand at 5126.537 kb on - strand at 5126.537 kb on - strand at 5126.539 kb on - strand at 5126.539 kb on - strand at 5126.880 kb on + strand, within AO356_22965 at 5126.913 kb on + strand, within AO356_22965 at 5127.123 kb on - strand, within AO356_22965 at 5127.169 kb on + strand, within AO356_22965 at 5127.169 kb on + strand, within AO356_22965 at 5127.169 kb on + strand, within AO356_22965 at 5127.169 kb on + strand, within AO356_22965 at 5127.169 kb on + strand, within AO356_22965 at 5127.169 kb on + strand, within AO356_22965 at 5127.170 kb on - strand, within AO356_22965 at 5127.328 kb on + strand, within AO356_22965 at 5127.328 kb on + strand, within AO356_22965 at 5127.329 kb on - strand, within AO356_22965 at 5127.329 kb on - strand, within AO356_22965 at 5127.419 kb on + strand, within AO356_22965 at 5127.426 kb on + strand, within AO356_22965 at 5127.427 kb on - strand, within AO356_22965 at 5127.542 kb on + strand at 5127.608 kb on + strand at 5127.609 kb on - strand at 5127.679 kb on + strand at 5127.679 kb on + strand at 5127.679 kb on + strand at 5127.680 kb on - strand at 5127.680 kb on - strand at 5127.742 kb on + strand, within AO356_22970 at 5127.742 kb on + strand, within AO356_22970 at 5127.742 kb on + strand, within AO356_22970 at 5127.743 kb on - strand, within AO356_22970 at 5128.007 kb on - strand, within AO356_22970 at 5128.066 kb on + strand, within AO356_22970 at 5128.066 kb on + strand, within AO356_22970 at 5128.067 kb on - strand, within AO356_22970 at 5128.162 kb on + strand, within AO356_22970 at 5128.163 kb on - strand, within AO356_22970 at 5128.280 kb on - strand, within AO356_22970 at 5128.474 kb on + strand, within AO356_22970
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,125,706 + -1.8 5,125,706 + -1.2 5,125,707 - +0.5 5,125,707 - +0.3 5,125,707 - -0.7 5,125,995 + AO356_22960 0.38 -2.7 5,126,032 + AO356_22960 0.42 -1.0 5,126,032 + AO356_22960 0.42 +0.5 5,126,033 - AO356_22960 0.42 +1.2 5,126,218 + AO356_22960 0.66 -1.9 5,126,219 - AO356_22960 0.66 +0.9 5,126,219 - AO356_22960 0.66 -1.0 5,126,308 + AO356_22960 0.77 -1.6 5,126,309 - AO356_22960 0.77 +0.9 5,126,309 - AO356_22960 0.77 +1.4 5,126,309 - AO356_22960 0.77 -0.8 5,126,347 + AO356_22960 0.82 +2.3 5,126,348 - AO356_22960 0.82 -1.9 5,126,348 - AO356_22960 0.82 +0.2 5,126,389 + AO356_22960 0.87 +0.3 5,126,536 + +0.3 5,126,537 - -1.1 5,126,537 - -0.5 5,126,537 - +0.4 5,126,539 - -0.2 5,126,539 - -0.3 5,126,880 + AO356_22965 0.30 -3.3 5,126,913 + AO356_22965 0.34 +0.6 5,127,123 - AO356_22965 0.56 +0.9 5,127,169 + AO356_22965 0.61 +0.5 5,127,169 + AO356_22965 0.61 -1.0 5,127,169 + AO356_22965 0.61 +1.0 5,127,169 + AO356_22965 0.61 -2.5 5,127,169 + AO356_22965 0.61 +0.2 5,127,169 + AO356_22965 0.61 -1.7 5,127,170 - AO356_22965 0.61 -0.2 5,127,328 + AO356_22965 0.78 +0.3 5,127,328 + AO356_22965 0.78 +0.2 5,127,329 - AO356_22965 0.78 -0.5 5,127,329 - AO356_22965 0.78 -2.2 5,127,419 + AO356_22965 0.88 +0.2 5,127,426 + AO356_22965 0.89 -0.6 5,127,427 - AO356_22965 0.89 +0.6 5,127,542 + +0.8 5,127,608 + -0.2 5,127,609 - -0.1 5,127,679 + -0.8 5,127,679 + +0.7 5,127,679 + -2.1 5,127,680 - +1.0 5,127,680 - +0.8 5,127,742 + AO356_22970 0.13 -1.6 5,127,742 + AO356_22970 0.13 +1.5 5,127,742 + AO356_22970 0.13 -0.5 5,127,743 - AO356_22970 0.13 -0.5 5,128,007 - AO356_22970 0.39 -0.0 5,128,066 + AO356_22970 0.45 +0.6 5,128,066 + AO356_22970 0.45 -0.8 5,128,067 - AO356_22970 0.45 +1.8 5,128,162 + AO356_22970 0.54 -1.4 5,128,163 - AO356_22970 0.54 -1.9 5,128,280 - AO356_22970 0.65 -2.4 5,128,474 + AO356_22970 0.85 -0.2
Or see this region's nucleotide sequence