Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_19995 and AO356_20000 are separated by 165 nucleotides AO356_20000 and AO356_20005 are separated by 22 nucleotides AO356_20005 and AO356_20010 are separated by 56 nucleotides
AO356_19995: AO356_19995 - hypothetical protein, at 4,476,332 to 4,477,180
_19995
AO356_20000: AO356_20000 - hypothetical protein, at 4,477,346 to 4,477,615
_20000
AO356_20005: AO356_20005 - hypothetical protein, at 4,477,638 to 4,478,351
_20005
AO356_20010: AO356_20010 - secretin, at 4,478,408 to 4,479,631
_20010
Position (kb)
4477
4478
4479 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4477.235 kb on + strand at 4477.235 kb on + strand at 4477.235 kb on + strand at 4477.288 kb on + strand at 4477.288 kb on + strand at 4477.288 kb on + strand at 4477.288 kb on + strand at 4477.289 kb on - strand at 4477.289 kb on - strand at 4477.639 kb on + strand at 4477.816 kb on + strand, within AO356_20005 at 4477.817 kb on - strand, within AO356_20005 at 4477.817 kb on - strand, within AO356_20005 at 4477.924 kb on + strand, within AO356_20005 at 4477.925 kb on - strand, within AO356_20005 at 4477.925 kb on - strand, within AO356_20005 at 4477.991 kb on - strand, within AO356_20005 at 4478.086 kb on + strand, within AO356_20005 at 4478.087 kb on - strand, within AO356_20005 at 4478.125 kb on - strand, within AO356_20005 at 4478.194 kb on + strand, within AO356_20005 at 4478.195 kb on - strand, within AO356_20005 at 4478.195 kb on - strand, within AO356_20005 at 4478.196 kb on + strand, within AO356_20005 at 4478.197 kb on - strand, within AO356_20005 at 4478.197 kb on - strand, within AO356_20005 at 4478.457 kb on + strand at 4478.457 kb on + strand at 4478.457 kb on + strand at 4478.457 kb on + strand at 4478.458 kb on - strand at 4478.458 kb on - strand at 4478.458 kb on - strand at 4478.458 kb on - strand at 4478.458 kb on - strand at 4478.461 kb on + strand at 4478.461 kb on + strand at 4478.461 kb on + strand at 4478.461 kb on + strand at 4478.461 kb on + strand at 4478.461 kb on + strand at 4478.461 kb on + strand at 4478.461 kb on + strand at 4478.462 kb on - strand at 4478.462 kb on - strand at 4478.462 kb on - strand at 4478.462 kb on - strand at 4478.462 kb on - strand at 4478.867 kb on + strand, within AO356_20010 at 4478.867 kb on + strand, within AO356_20010 at 4478.868 kb on - strand, within AO356_20010 at 4478.868 kb on - strand, within AO356_20010 at 4478.868 kb on - strand, within AO356_20010 at 4478.998 kb on + strand, within AO356_20010 at 4478.999 kb on - strand, within AO356_20010 at 4479.001 kb on - strand, within AO356_20010 at 4479.047 kb on - strand, within AO356_20010 at 4479.080 kb on - strand, within AO356_20010 at 4479.330 kb on - strand, within AO356_20010
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,477,235 + -1.7 4,477,235 + +2.3 4,477,235 + -1.4 4,477,288 + -0.2 4,477,288 + +0.9 4,477,288 + +0.4 4,477,288 + -0.1 4,477,289 - -1.9 4,477,289 - -0.2 4,477,639 + +2.8 4,477,816 + AO356_20005 0.25 -0.5 4,477,817 - AO356_20005 0.25 +0.4 4,477,817 - AO356_20005 0.25 -1.6 4,477,924 + AO356_20005 0.40 +0.5 4,477,925 - AO356_20005 0.40 +0.2 4,477,925 - AO356_20005 0.40 -2.7 4,477,991 - AO356_20005 0.49 -1.6 4,478,086 + AO356_20005 0.63 -1.3 4,478,087 - AO356_20005 0.63 -1.9 4,478,125 - AO356_20005 0.68 +0.5 4,478,194 + AO356_20005 0.78 +0.1 4,478,195 - AO356_20005 0.78 +0.2 4,478,195 - AO356_20005 0.78 +0.6 4,478,196 + AO356_20005 0.78 +1.2 4,478,197 - AO356_20005 0.78 -0.6 4,478,197 - AO356_20005 0.78 +3.4 4,478,457 + +0.6 4,478,457 + +0.6 4,478,457 + +1.9 4,478,457 + -0.6 4,478,458 - +1.4 4,478,458 - -1.4 4,478,458 - +1.2 4,478,458 - -1.3 4,478,458 - -0.3 4,478,461 + -1.8 4,478,461 + -1.2 4,478,461 + -1.4 4,478,461 + +0.9 4,478,461 + -0.8 4,478,461 + -0.1 4,478,461 + -0.3 4,478,461 + +2.0 4,478,462 - +0.9 4,478,462 - +0.3 4,478,462 - -0.4 4,478,462 - -0.8 4,478,462 - -0.4 4,478,867 + AO356_20010 0.38 +1.1 4,478,867 + AO356_20010 0.38 -0.2 4,478,868 - AO356_20010 0.38 -1.2 4,478,868 - AO356_20010 0.38 +0.3 4,478,868 - AO356_20010 0.38 +2.0 4,478,998 + AO356_20010 0.48 +0.9 4,478,999 - AO356_20010 0.48 -2.2 4,479,001 - AO356_20010 0.48 +0.9 4,479,047 - AO356_20010 0.52 +1.4 4,479,080 - AO356_20010 0.55 +1.5 4,479,330 - AO356_20010 0.75 -1.1
Or see this region's nucleotide sequence