Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_19600 and AO356_19605 overlap by 11 nucleotides AO356_19605 and AO356_19610 are separated by 143 nucleotides
AO356_19600: AO356_19600 - pyruvate kinase, at 4,394,541 to 4,395,956
_19600
AO356_19605: AO356_19605 - hydroxypyruvate reductase, at 4,395,946 to 4,397,226
_19605
AO356_19610: AO356_19610 - 2-hydroxy-3-oxopropionate reductase, at 4,397,370 to 4,398,260
_19610
Position (kb) 4395
4396
4397
4398 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4395.773 kb on - strand, within AO356_19600 at 4395.944 kb on + strand at 4396.050 kb on - strand at 4396.050 kb on - strand at 4396.190 kb on + strand, within AO356_19605 at 4396.190 kb on + strand, within AO356_19605 at 4396.190 kb on + strand, within AO356_19605 at 4396.190 kb on + strand, within AO356_19605 at 4396.190 kb on + strand, within AO356_19605 at 4396.190 kb on + strand, within AO356_19605 at 4396.190 kb on + strand, within AO356_19605 at 4396.191 kb on - strand, within AO356_19605 at 4396.191 kb on - strand, within AO356_19605 at 4396.614 kb on + strand, within AO356_19605 at 4396.614 kb on + strand, within AO356_19605 at 4396.615 kb on - strand, within AO356_19605 at 4396.685 kb on + strand, within AO356_19605 at 4396.686 kb on - strand, within AO356_19605 at 4396.686 kb on - strand, within AO356_19605 at 4396.691 kb on + strand, within AO356_19605 at 4396.691 kb on + strand, within AO356_19605 at 4396.691 kb on + strand, within AO356_19605 at 4396.691 kb on + strand, within AO356_19605 at 4396.692 kb on - strand, within AO356_19605 at 4396.990 kb on + strand, within AO356_19605 at 4397.141 kb on + strand at 4397.142 kb on - strand at 4397.244 kb on + strand at 4397.245 kb on - strand at 4397.247 kb on + strand at 4397.248 kb on - strand at 4397.248 kb on - strand at 4397.250 kb on + strand at 4397.250 kb on + strand at 4397.250 kb on + strand at 4397.250 kb on + strand at 4397.250 kb on + strand at 4397.251 kb on - strand at 4397.251 kb on - strand at 4397.251 kb on - strand at 4397.251 kb on - strand at 4397.300 kb on + strand at 4397.371 kb on + strand at 4397.371 kb on + strand at 4397.372 kb on - strand at 4397.372 kb on - strand at 4397.399 kb on + strand at 4397.399 kb on + strand at 4397.399 kb on + strand at 4397.400 kb on - strand at 4397.608 kb on - strand, within AO356_19610 at 4397.914 kb on + strand, within AO356_19610 at 4397.915 kb on - strand, within AO356_19610 at 4397.915 kb on - strand, within AO356_19610
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,395,773 - AO356_19600 0.87 -0.8 4,395,944 + -1.6 4,396,050 - +0.7 4,396,050 - +0.4 4,396,190 + AO356_19605 0.19 +1.1 4,396,190 + AO356_19605 0.19 +1.4 4,396,190 + AO356_19605 0.19 -1.6 4,396,190 + AO356_19605 0.19 +0.7 4,396,190 + AO356_19605 0.19 -2.0 4,396,190 + AO356_19605 0.19 +0.3 4,396,190 + AO356_19605 0.19 +0.2 4,396,191 - AO356_19605 0.19 -1.5 4,396,191 - AO356_19605 0.19 -0.6 4,396,614 + AO356_19605 0.52 +0.4 4,396,614 + AO356_19605 0.52 -0.7 4,396,615 - AO356_19605 0.52 -1.5 4,396,685 + AO356_19605 0.58 +1.7 4,396,686 - AO356_19605 0.58 +0.4 4,396,686 - AO356_19605 0.58 -1.1 4,396,691 + AO356_19605 0.58 +0.6 4,396,691 + AO356_19605 0.58 -1.4 4,396,691 + AO356_19605 0.58 +1.7 4,396,691 + AO356_19605 0.58 -0.1 4,396,692 - AO356_19605 0.58 +0.3 4,396,990 + AO356_19605 0.81 -0.9 4,397,141 + -1.1 4,397,142 - -2.4 4,397,244 + -0.9 4,397,245 - +0.0 4,397,247 + +0.9 4,397,248 - -1.1 4,397,248 - -1.8 4,397,250 + +0.7 4,397,250 + +0.0 4,397,250 + +0.1 4,397,250 + +0.5 4,397,250 + -0.5 4,397,251 - +0.6 4,397,251 - +0.6 4,397,251 - +1.3 4,397,251 - +1.1 4,397,300 + +0.5 4,397,371 + +1.1 4,397,371 + +1.0 4,397,372 - -2.0 4,397,372 - +1.5 4,397,399 + -0.4 4,397,399 + +2.7 4,397,399 + +0.5 4,397,400 - -1.8 4,397,608 - AO356_19610 0.27 -0.8 4,397,914 + AO356_19610 0.61 -1.9 4,397,915 - AO356_19610 0.61 -1.8 4,397,915 - AO356_19610 0.61 -1.5
Or see this region's nucleotide sequence