Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_19340 and AO356_19345 are separated by 319 nucleotides AO356_19345 and AO356_19350 overlap by 1 nucleotides AO356_19350 and AO356_19355 are separated by 124 nucleotides AO356_19355 and AO356_19360 are separated by 34 nucleotides
AO356_19340: AO356_19340 - NAD(P)H-quinone oxidoreductase, at 4,343,002 to 4,343,964
_19340
AO356_19345: AO356_19345 - hypothetical protein, at 4,344,284 to 4,344,919
_19345
AO356_19350: AO356_19350 - hypothetical protein, at 4,344,919 to 4,345,707
_19350
AO356_19355: AO356_19355 - HAD family hydrolase, at 4,345,832 to 4,346,485
_19355
AO356_19360: AO356_19360 - spermidine/putrescine ABC transporter ATP-binding protein, at 4,346,520 to 4,347,509
_19360
Position (kb)
4344
4345
4346 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4344.076 kb on + strand at 4344.076 kb on + strand at 4344.076 kb on + strand at 4344.076 kb on + strand at 4344.077 kb on - strand at 4344.077 kb on - strand at 4344.108 kb on + strand at 4344.221 kb on + strand at 4344.221 kb on + strand at 4344.222 kb on - strand at 4344.222 kb on - strand at 4344.222 kb on - strand at 4344.222 kb on - strand at 4344.337 kb on + strand at 4344.337 kb on + strand at 4344.338 kb on - strand at 4344.338 kb on - strand at 4344.338 kb on - strand at 4344.423 kb on - strand, within AO356_19345 at 4344.827 kb on + strand, within AO356_19345 at 4344.828 kb on - strand, within AO356_19345 at 4344.852 kb on + strand, within AO356_19345 at 4344.852 kb on + strand, within AO356_19345 at 4344.852 kb on + strand, within AO356_19345 at 4344.852 kb on + strand, within AO356_19345 at 4344.853 kb on - strand, within AO356_19345 at 4344.853 kb on - strand, within AO356_19345 at 4344.872 kb on - strand at 4344.872 kb on - strand at 4344.888 kb on + strand at 4344.888 kb on + strand at 4344.888 kb on + strand at 4344.888 kb on + strand at 4344.889 kb on - strand at 4344.889 kb on - strand at 4344.909 kb on + strand at 4344.909 kb on + strand at 4344.909 kb on + strand at 4344.910 kb on - strand at 4344.910 kb on - strand at 4344.910 kb on - strand at 4345.189 kb on + strand, within AO356_19350 at 4345.190 kb on - strand, within AO356_19350 at 4345.261 kb on + strand, within AO356_19350 at 4345.262 kb on - strand, within AO356_19350 at 4345.262 kb on - strand, within AO356_19350 at 4345.262 kb on - strand, within AO356_19350 at 4345.359 kb on + strand, within AO356_19350 at 4345.399 kb on + strand, within AO356_19350 at 4345.400 kb on - strand, within AO356_19350 at 4345.468 kb on + strand, within AO356_19350 at 4345.469 kb on - strand, within AO356_19350 at 4345.522 kb on + strand, within AO356_19350 at 4345.522 kb on + strand, within AO356_19350 at 4345.523 kb on - strand, within AO356_19350 at 4345.705 kb on + strand at 4345.720 kb on + strand at 4345.720 kb on + strand at 4345.720 kb on + strand at 4345.720 kb on + strand at 4345.721 kb on - strand at 4345.772 kb on + strand at 4345.843 kb on + strand at 4345.843 kb on + strand at 4345.843 kb on + strand at 4345.956 kb on + strand, within AO356_19355 at 4345.956 kb on + strand, within AO356_19355 at 4346.031 kb on + strand, within AO356_19355 at 4346.032 kb on - strand, within AO356_19355 at 4346.062 kb on - strand, within AO356_19355 at 4346.316 kb on + strand, within AO356_19355 at 4346.316 kb on + strand, within AO356_19355 at 4346.316 kb on + strand, within AO356_19355 at 4346.317 kb on - strand, within AO356_19355 at 4346.317 kb on - strand, within AO356_19355 at 4346.317 kb on - strand, within AO356_19355 at 4346.317 kb on - strand, within AO356_19355 at 4346.346 kb on + strand, within AO356_19355 at 4346.346 kb on + strand, within AO356_19355
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,344,076 + +0.2 4,344,076 + +0.1 4,344,076 + +0.4 4,344,076 + -1.2 4,344,077 - -2.1 4,344,077 - +1.7 4,344,108 + -0.5 4,344,221 + +0.6 4,344,221 + +1.1 4,344,222 - +1.1 4,344,222 - +1.7 4,344,222 - +0.7 4,344,222 - -0.1 4,344,337 + +0.7 4,344,337 + +0.0 4,344,338 - +1.4 4,344,338 - +1.1 4,344,338 - +1.1 4,344,423 - AO356_19345 0.22 +0.7 4,344,827 + AO356_19345 0.85 -0.6 4,344,828 - AO356_19345 0.86 -1.5 4,344,852 + AO356_19345 0.89 -1.2 4,344,852 + AO356_19345 0.89 +0.4 4,344,852 + AO356_19345 0.89 -0.4 4,344,852 + AO356_19345 0.89 +1.5 4,344,853 - AO356_19345 0.89 -2.4 4,344,853 - AO356_19345 0.89 +0.3 4,344,872 - -0.1 4,344,872 - +0.8 4,344,888 + +0.7 4,344,888 + +0.0 4,344,888 + +0.5 4,344,888 + +2.4 4,344,889 - +0.1 4,344,889 - +1.4 4,344,909 + +0.0 4,344,909 + -1.5 4,344,909 + -1.8 4,344,910 - +1.3 4,344,910 - +0.1 4,344,910 - +0.4 4,345,189 + AO356_19350 0.34 -1.4 4,345,190 - AO356_19350 0.34 -2.5 4,345,261 + AO356_19350 0.43 +0.2 4,345,262 - AO356_19350 0.43 +2.2 4,345,262 - AO356_19350 0.43 +0.6 4,345,262 - AO356_19350 0.43 -0.8 4,345,359 + AO356_19350 0.56 -2.6 4,345,399 + AO356_19350 0.61 -3.4 4,345,400 - AO356_19350 0.61 -1.7 4,345,468 + AO356_19350 0.70 +1.3 4,345,469 - AO356_19350 0.70 +0.4 4,345,522 + AO356_19350 0.76 +0.4 4,345,522 + AO356_19350 0.76 +1.6 4,345,523 - AO356_19350 0.77 -1.8 4,345,705 + +0.2 4,345,720 + +0.4 4,345,720 + -0.9 4,345,720 + +1.4 4,345,720 + -0.7 4,345,721 - -1.2 4,345,772 + +0.2 4,345,843 + -0.4 4,345,843 + -0.9 4,345,843 + -0.2 4,345,956 + AO356_19355 0.19 -2.5 4,345,956 + AO356_19355 0.19 -1.4 4,346,031 + AO356_19355 0.30 -0.3 4,346,032 - AO356_19355 0.31 +0.2 4,346,062 - AO356_19355 0.35 -0.7 4,346,316 + AO356_19355 0.74 +0.0 4,346,316 + AO356_19355 0.74 -0.1 4,346,316 + AO356_19355 0.74 +2.0 4,346,317 - AO356_19355 0.74 +2.7 4,346,317 - AO356_19355 0.74 +0.2 4,346,317 - AO356_19355 0.74 -1.0 4,346,317 - AO356_19355 0.74 -1.3 4,346,346 + AO356_19355 0.79 +0.2 4,346,346 + AO356_19355 0.79 +0.6
Or see this region's nucleotide sequence