Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_18355 and AO356_18360 overlap by 4 nucleotides AO356_18360 and AO356_18365 overlap by 14 nucleotides AO356_18365 and AO356_18370 overlap by 4 nucleotides AO356_18370 and AO356_18375 are separated by 131 nucleotides
AO356_18355: AO356_18355 - electron transporter RnfG, at 4,132,792 to 4,133,400
_18355
AO356_18360: AO356_18360 - electron transporter RnfD, at 4,133,397 to 4,134,377
_18360
AO356_18365: AO356_18365 - (4Fe-4S)-binding protein, at 4,134,364 to 4,135,377
_18365
AO356_18370: AO356_18370 - NADH:quinone oxidoreductase, at 4,135,374 to 4,135,946
_18370
AO356_18375: AO356_18375 - methionine--tRNA ligase, at 4,136,078 to 4,138,123
_18375
Position (kb) 4134
4135
4136 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4133.696 kb on + strand, within AO356_18360 at 4133.697 kb on - strand, within AO356_18360 at 4133.981 kb on + strand, within AO356_18360 at 4133.981 kb on + strand, within AO356_18360 at 4133.981 kb on + strand, within AO356_18360 at 4133.981 kb on + strand, within AO356_18360 at 4133.981 kb on + strand, within AO356_18360 at 4133.981 kb on + strand, within AO356_18360 at 4133.982 kb on - strand, within AO356_18360 at 4133.982 kb on - strand, within AO356_18360 at 4133.982 kb on - strand, within AO356_18360 at 4134.013 kb on + strand, within AO356_18360 at 4134.014 kb on - strand, within AO356_18360 at 4134.121 kb on + strand, within AO356_18360 at 4134.121 kb on + strand, within AO356_18360 at 4134.121 kb on + strand, within AO356_18360 at 4134.265 kb on - strand, within AO356_18360 at 4134.265 kb on - strand, within AO356_18360 at 4134.773 kb on - strand, within AO356_18365 at 4135.359 kb on + strand at 4135.360 kb on - strand at 4135.440 kb on + strand, within AO356_18370 at 4135.440 kb on + strand, within AO356_18370 at 4135.441 kb on - strand, within AO356_18370 at 4135.441 kb on - strand, within AO356_18370 at 4135.441 kb on - strand, within AO356_18370 at 4135.469 kb on + strand, within AO356_18370 at 4135.975 kb on - strand at 4135.975 kb on - strand at 4135.982 kb on + strand at 4135.983 kb on - strand at 4135.983 kb on - strand at 4135.983 kb on - strand at 4135.983 kb on - strand at 4136.018 kb on + strand at 4136.018 kb on + strand at 4136.019 kb on - strand at 4136.019 kb on - strand at 4136.019 kb on - strand at 4136.019 kb on - strand at 4136.019 kb on - strand at 4136.080 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,133,696 + AO356_18360 0.30 +0.6 4,133,697 - AO356_18360 0.31 +0.8 4,133,981 + AO356_18360 0.60 +1.2 4,133,981 + AO356_18360 0.60 +0.5 4,133,981 + AO356_18360 0.60 +0.6 4,133,981 + AO356_18360 0.60 -0.7 4,133,981 + AO356_18360 0.60 -1.5 4,133,981 + AO356_18360 0.60 +0.1 4,133,982 - AO356_18360 0.60 -1.2 4,133,982 - AO356_18360 0.60 -0.6 4,133,982 - AO356_18360 0.60 +1.0 4,134,013 + AO356_18360 0.63 +0.5 4,134,014 - AO356_18360 0.63 +2.1 4,134,121 + AO356_18360 0.74 -1.3 4,134,121 + AO356_18360 0.74 -2.3 4,134,121 + AO356_18360 0.74 -1.0 4,134,265 - AO356_18360 0.88 -2.2 4,134,265 - AO356_18360 0.88 +0.2 4,134,773 - AO356_18365 0.40 -0.2 4,135,359 + -0.5 4,135,360 - -2.9 4,135,440 + AO356_18370 0.12 -0.8 4,135,440 + AO356_18370 0.12 +0.2 4,135,441 - AO356_18370 0.12 -2.2 4,135,441 - AO356_18370 0.12 +1.5 4,135,441 - AO356_18370 0.12 +0.6 4,135,469 + AO356_18370 0.17 -2.4 4,135,975 - -2.1 4,135,975 - +2.4 4,135,982 + -0.7 4,135,983 - -1.3 4,135,983 - -0.4 4,135,983 - -0.1 4,135,983 - -1.0 4,136,018 + -1.3 4,136,018 + +2.5 4,136,019 - -0.3 4,136,019 - +0.1 4,136,019 - -0.3 4,136,019 - -0.4 4,136,019 - -0.0 4,136,080 - +1.8
Or see this region's nucleotide sequence