Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_10430 and AO356_10435 are separated by 47 nucleotides AO356_10435 and AO356_10440 are separated by 205 nucleotides AO356_10440 and AO356_10445 are separated by 148 nucleotides AO356_10445 and AO356_10450 are separated by 1 nucleotides
AO356_10430: AO356_10430 - Fis family transcriptional regulator, at 2,353,755 to 2,354,690
_10430
AO356_10435: AO356_10435 - alpha/beta hydrolase, at 2,354,738 to 2,355,319
_10435
AO356_10440: AO356_10440 - hypothetical protein, at 2,355,525 to 2,355,779
_10440
AO356_10445: AO356_10445 - serine protease, at 2,355,928 to 2,356,452
_10445
AO356_10450: AO356_10450 - hypothetical protein, at 2,356,454 to 2,357,215
_10450
Position (kb)
2355
2356 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2354.526 kb on + strand, within AO356_10430 at 2354.526 kb on + strand, within AO356_10430 at 2354.527 kb on - strand, within AO356_10430 at 2354.784 kb on + strand at 2354.784 kb on + strand at 2354.785 kb on - strand at 2354.790 kb on + strand at 2354.790 kb on + strand at 2354.791 kb on - strand at 2354.791 kb on - strand at 2354.791 kb on - strand at 2354.836 kb on + strand, within AO356_10435 at 2354.836 kb on + strand, within AO356_10435 at 2354.837 kb on - strand, within AO356_10435 at 2354.837 kb on - strand, within AO356_10435 at 2354.837 kb on - strand, within AO356_10435 at 2354.837 kb on - strand, within AO356_10435 at 2354.837 kb on - strand, within AO356_10435 at 2355.297 kb on + strand at 2355.298 kb on - strand at 2355.353 kb on + strand at 2355.353 kb on + strand at 2355.353 kb on + strand at 2355.353 kb on + strand at 2355.354 kb on - strand at 2355.355 kb on + strand at 2355.355 kb on + strand at 2355.355 kb on + strand at 2355.355 kb on + strand at 2355.355 kb on + strand at 2355.355 kb on + strand at 2355.355 kb on + strand at 2355.356 kb on - strand at 2355.356 kb on - strand at 2355.356 kb on - strand at 2355.356 kb on - strand at 2355.356 kb on - strand at 2355.356 kb on - strand at 2355.369 kb on - strand at 2355.369 kb on - strand at 2355.422 kb on + strand at 2355.479 kb on + strand at 2355.479 kb on + strand at 2355.480 kb on - strand at 2355.480 kb on - strand at 2355.480 kb on - strand at 2355.480 kb on - strand at 2355.488 kb on + strand at 2355.488 kb on + strand at 2355.489 kb on - strand at 2355.489 kb on - strand at 2355.542 kb on + strand at 2355.577 kb on - strand, within AO356_10440 at 2355.577 kb on - strand, within AO356_10440 at 2355.577 kb on - strand, within AO356_10440 at 2355.577 kb on - strand, within AO356_10440 at 2355.577 kb on - strand, within AO356_10440 at 2355.577 kb on - strand, within AO356_10440 at 2355.577 kb on - strand, within AO356_10440 at 2355.577 kb on - strand, within AO356_10440 at 2355.577 kb on - strand, within AO356_10440 at 2355.577 kb on - strand, within AO356_10440 at 2355.828 kb on + strand at 2355.828 kb on + strand at 2355.828 kb on + strand at 2355.829 kb on - strand at 2355.893 kb on + strand at 2355.893 kb on + strand at 2355.894 kb on - strand at 2355.894 kb on - strand at 2355.894 kb on - strand at 2356.081 kb on + strand, within AO356_10445 at 2356.082 kb on - strand, within AO356_10445 at 2356.082 kb on - strand, within AO356_10445 at 2356.448 kb on + strand at 2356.536 kb on - strand, within AO356_10450 at 2356.560 kb on + strand, within AO356_10450 at 2356.561 kb on - strand, within AO356_10450 at 2356.614 kb on + strand, within AO356_10450 at 2356.614 kb on + strand, within AO356_10450 at 2356.718 kb on + strand, within AO356_10450 at 2356.718 kb on + strand, within AO356_10450 at 2356.718 kb on + strand, within AO356_10450 at 2356.718 kb on + strand, within AO356_10450 at 2356.718 kb on + strand, within AO356_10450 at 2356.719 kb on - strand, within AO356_10450 at 2356.719 kb on - strand, within AO356_10450
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,354,526 + AO356_10430 0.82 +1.6 2,354,526 + AO356_10430 0.82 -0.8 2,354,527 - AO356_10430 0.82 +1.0 2,354,784 + +2.4 2,354,784 + -0.3 2,354,785 - -2.9 2,354,790 + -0.0 2,354,790 + +0.8 2,354,791 - +1.0 2,354,791 - -0.6 2,354,791 - +0.2 2,354,836 + AO356_10435 0.17 +0.0 2,354,836 + AO356_10435 0.17 +0.1 2,354,837 - AO356_10435 0.17 +1.1 2,354,837 - AO356_10435 0.17 +0.2 2,354,837 - AO356_10435 0.17 +0.4 2,354,837 - AO356_10435 0.17 +0.6 2,354,837 - AO356_10435 0.17 -0.5 2,355,297 + +1.7 2,355,298 - -1.8 2,355,353 + -1.2 2,355,353 + -2.2 2,355,353 + -3.0 2,355,353 + -2.6 2,355,354 - -2.8 2,355,355 + +0.6 2,355,355 + -1.3 2,355,355 + -1.0 2,355,355 + +0.7 2,355,355 + +0.4 2,355,355 + -2.0 2,355,355 + -0.6 2,355,356 - +0.5 2,355,356 - -0.4 2,355,356 - -0.6 2,355,356 - +2.0 2,355,356 - -0.1 2,355,356 - -1.7 2,355,369 - +1.2 2,355,369 - +0.8 2,355,422 + +1.0 2,355,479 + +1.9 2,355,479 + +1.5 2,355,480 - -0.1 2,355,480 - +0.7 2,355,480 - +0.5 2,355,480 - -0.5 2,355,488 + +0.7 2,355,488 + -1.0 2,355,489 - +1.1 2,355,489 - +1.7 2,355,542 + -0.1 2,355,577 - AO356_10440 0.20 -0.6 2,355,577 - AO356_10440 0.20 +0.0 2,355,577 - AO356_10440 0.20 +1.6 2,355,577 - AO356_10440 0.20 +0.8 2,355,577 - AO356_10440 0.20 +0.6 2,355,577 - AO356_10440 0.20 +1.1 2,355,577 - AO356_10440 0.20 +0.7 2,355,577 - AO356_10440 0.20 +1.1 2,355,577 - AO356_10440 0.20 -0.9 2,355,577 - AO356_10440 0.20 -2.9 2,355,828 + +1.8 2,355,828 + -1.3 2,355,828 + +0.8 2,355,829 - -1.0 2,355,893 + -1.3 2,355,893 + -0.8 2,355,894 - -1.7 2,355,894 - +0.2 2,355,894 - -1.8 2,356,081 + AO356_10445 0.29 -0.2 2,356,082 - AO356_10445 0.29 +1.2 2,356,082 - AO356_10445 0.29 +0.5 2,356,448 + -0.5 2,356,536 - AO356_10450 0.11 -0.8 2,356,560 + AO356_10450 0.14 +0.8 2,356,561 - AO356_10450 0.14 -2.1 2,356,614 + AO356_10450 0.21 -0.3 2,356,614 + AO356_10450 0.21 +0.2 2,356,718 + AO356_10450 0.35 -0.3 2,356,718 + AO356_10450 0.35 -0.0 2,356,718 + AO356_10450 0.35 -1.2 2,356,718 + AO356_10450 0.35 -2.3 2,356,718 + AO356_10450 0.35 +0.7 2,356,719 - AO356_10450 0.35 -0.9 2,356,719 - AO356_10450 0.35 -1.8
Or see this region's nucleotide sequence