Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_04645 and AO356_04650 are separated by 15 nucleotides AO356_04650 and AO356_04655 overlap by 4 nucleotides AO356_04655 and AO356_04660 are separated by 13 nucleotides
AO356_04645: AO356_04645 - lipid kinase, at 1,046,266 to 1,047,333
_04645
AO356_04650: AO356_04650 - lipid kinase, at 1,047,349 to 1,048,164
_04650
AO356_04655: AO356_04655 - lauroyl acyltransferase, at 1,048,161 to 1,048,949
_04655
AO356_04660: AO356_04660 - urea carboxylase, at 1,048,963 to 1,049,688
_04660
Position (kb)
1047
1048
1049 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1046.374 kb on + strand, within AO356_04645 at 1046.374 kb on + strand, within AO356_04645 at 1046.374 kb on + strand, within AO356_04645 at 1046.374 kb on + strand, within AO356_04645 at 1046.375 kb on - strand, within AO356_04645 at 1046.476 kb on + strand, within AO356_04645 at 1046.476 kb on + strand, within AO356_04645 at 1046.477 kb on - strand, within AO356_04645 at 1046.497 kb on + strand, within AO356_04645 at 1046.497 kb on + strand, within AO356_04645 at 1046.498 kb on - strand, within AO356_04645 at 1046.702 kb on - strand, within AO356_04645 at 1047.301 kb on + strand at 1047.301 kb on + strand at 1047.301 kb on + strand at 1047.301 kb on + strand at 1047.302 kb on - strand at 1047.302 kb on - strand at 1047.302 kb on - strand at 1047.302 kb on - strand at 1047.302 kb on - strand at 1047.302 kb on - strand at 1047.305 kb on + strand at 1047.305 kb on + strand at 1047.305 kb on + strand at 1047.305 kb on + strand at 1047.305 kb on + strand at 1047.306 kb on - strand at 1047.306 kb on - strand at 1047.306 kb on - strand at 1047.306 kb on - strand at 1047.495 kb on - strand, within AO356_04650 at 1047.495 kb on - strand, within AO356_04650 at 1047.553 kb on + strand, within AO356_04650 at 1047.554 kb on - strand, within AO356_04650 at 1047.554 kb on - strand, within AO356_04650 at 1047.918 kb on - strand, within AO356_04650 at 1047.918 kb on - strand, within AO356_04650 at 1048.066 kb on + strand, within AO356_04650 at 1048.067 kb on - strand, within AO356_04650 at 1048.067 kb on - strand, within AO356_04650 at 1048.144 kb on + strand at 1048.145 kb on - strand at 1048.145 kb on - strand at 1048.307 kb on - strand, within AO356_04655 at 1048.404 kb on + strand, within AO356_04655 at 1048.565 kb on + strand, within AO356_04655 at 1048.566 kb on - strand, within AO356_04655 at 1048.566 kb on - strand, within AO356_04655 at 1048.566 kb on - strand, within AO356_04655 at 1048.566 kb on - strand, within AO356_04655 at 1048.569 kb on + strand, within AO356_04655 at 1048.840 kb on - strand, within AO356_04655 at 1048.857 kb on + strand, within AO356_04655 at 1048.857 kb on + strand, within AO356_04655 at 1048.857 kb on + strand, within AO356_04655 at 1048.857 kb on + strand, within AO356_04655 at 1048.858 kb on - strand, within AO356_04655 at 1048.986 kb on + strand at 1049.103 kb on - strand, within AO356_04660
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,046,374 + AO356_04645 0.10 +0.1 1,046,374 + AO356_04645 0.10 -1.8 1,046,374 + AO356_04645 0.10 -2.4 1,046,374 + AO356_04645 0.10 +1.7 1,046,375 - AO356_04645 0.10 +0.1 1,046,476 + AO356_04645 0.20 -0.2 1,046,476 + AO356_04645 0.20 +2.1 1,046,477 - AO356_04645 0.20 +0.5 1,046,497 + AO356_04645 0.22 +0.2 1,046,497 + AO356_04645 0.22 +2.4 1,046,498 - AO356_04645 0.22 +1.7 1,046,702 - AO356_04645 0.41 +1.3 1,047,301 + +0.3 1,047,301 + -0.8 1,047,301 + +1.3 1,047,301 + +1.1 1,047,302 - -0.1 1,047,302 - +1.4 1,047,302 - -0.6 1,047,302 - +0.4 1,047,302 - +0.5 1,047,302 - +0.8 1,047,305 + -0.1 1,047,305 + +1.3 1,047,305 + +1.6 1,047,305 + +1.0 1,047,305 + -1.1 1,047,306 - +2.7 1,047,306 - +1.3 1,047,306 - +0.4 1,047,306 - +0.8 1,047,495 - AO356_04650 0.18 +0.2 1,047,495 - AO356_04650 0.18 +0.3 1,047,553 + AO356_04650 0.25 +0.5 1,047,554 - AO356_04650 0.25 +1.2 1,047,554 - AO356_04650 0.25 +0.3 1,047,918 - AO356_04650 0.70 -0.1 1,047,918 - AO356_04650 0.70 -0.2 1,048,066 + AO356_04650 0.88 -0.4 1,048,067 - AO356_04650 0.88 +0.5 1,048,067 - AO356_04650 0.88 +0.3 1,048,144 + +2.0 1,048,145 - -1.1 1,048,145 - +1.0 1,048,307 - AO356_04655 0.19 -0.3 1,048,404 + AO356_04655 0.31 +1.9 1,048,565 + AO356_04655 0.51 +1.0 1,048,566 - AO356_04655 0.51 +2.1 1,048,566 - AO356_04655 0.51 -0.6 1,048,566 - AO356_04655 0.51 -1.4 1,048,566 - AO356_04655 0.51 +0.5 1,048,569 + AO356_04655 0.52 +0.5 1,048,840 - AO356_04655 0.86 -0.7 1,048,857 + AO356_04655 0.88 -1.2 1,048,857 + AO356_04655 0.88 -0.2 1,048,857 + AO356_04655 0.88 +0.8 1,048,857 + AO356_04655 0.88 -4.0 1,048,858 - AO356_04655 0.88 -0.9 1,048,986 + -1.5 1,049,103 - AO356_04660 0.19 -2.6
Or see this region's nucleotide sequence