Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_04515 and AO356_04520 are separated by 27 nucleotides AO356_04520 and AO356_04525 are separated by 4 nucleotides AO356_04525 and AO356_04530 are separated by 17 nucleotides
AO356_04515: AO356_04515 - phosphoribosylaminoimidazole-succinocarboxamide synthase, at 1,019,894 to 1,020,607
_04515
AO356_04520: AO356_04520 - MBL fold metallo-hydrolase, at 1,020,635 to 1,021,393
_04520
AO356_04525: AO356_04525 - hypothetical protein, at 1,021,398 to 1,022,513
_04525
AO356_04530: AO356_04530 - 4-hydroxy-tetrahydrodipicolinate synthase, at 1,022,531 to 1,023,409
_04530
Position (kb)
1021
1022
1023 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1020.624 kb on + strand at 1020.624 kb on + strand at 1020.625 kb on - strand at 1020.625 kb on - strand at 1020.625 kb on - strand at 1020.625 kb on - strand at 1020.625 kb on - strand at 1020.625 kb on - strand at 1020.625 kb on - strand at 1020.629 kb on - strand at 1020.629 kb on - strand at 1020.874 kb on - strand, within AO356_04520 at 1020.874 kb on - strand, within AO356_04520 at 1020.880 kb on - strand, within AO356_04520 at 1020.880 kb on - strand, within AO356_04520 at 1020.880 kb on - strand, within AO356_04520 at 1020.880 kb on - strand, within AO356_04520 at 1020.970 kb on - strand, within AO356_04520 at 1021.019 kb on + strand, within AO356_04520 at 1021.020 kb on - strand, within AO356_04520 at 1021.020 kb on - strand, within AO356_04520 at 1021.020 kb on - strand, within AO356_04520 at 1021.020 kb on - strand, within AO356_04520 at 1021.020 kb on - strand, within AO356_04520 at 1021.020 kb on - strand, within AO356_04520 at 1021.020 kb on - strand, within AO356_04520 at 1021.020 kb on - strand, within AO356_04520 at 1021.020 kb on - strand, within AO356_04520 at 1021.020 kb on - strand, within AO356_04520 at 1021.024 kb on - strand, within AO356_04520 at 1021.024 kb on - strand, within AO356_04520 at 1021.024 kb on - strand, within AO356_04520 at 1021.024 kb on - strand, within AO356_04520 at 1021.024 kb on - strand, within AO356_04520 at 1021.024 kb on - strand, within AO356_04520 at 1021.024 kb on - strand, within AO356_04520 at 1021.024 kb on - strand, within AO356_04520 at 1021.024 kb on - strand, within AO356_04520 at 1021.165 kb on - strand, within AO356_04520 at 1021.165 kb on - strand, within AO356_04520 at 1021.179 kb on + strand, within AO356_04520 at 1021.179 kb on + strand, within AO356_04520 at 1021.180 kb on - strand, within AO356_04520 at 1021.326 kb on + strand at 1021.326 kb on + strand at 1021.326 kb on + strand at 1021.326 kb on + strand at 1021.326 kb on + strand at 1021.327 kb on - strand at 1021.327 kb on - strand at 1021.327 kb on - strand at 1021.327 kb on - strand at 1021.327 kb on - strand at 1021.327 kb on - strand at 1021.330 kb on + strand at 1021.330 kb on + strand at 1021.330 kb on + strand at 1021.331 kb on - strand at 1021.331 kb on - strand at 1021.331 kb on - strand at 1021.331 kb on - strand at 1021.331 kb on - strand at 1021.331 kb on - strand at 1021.428 kb on + strand at 1021.428 kb on + strand at 1021.429 kb on - strand at 1021.429 kb on - strand at 1021.429 kb on - strand at 1021.633 kb on + strand, within AO356_04525 at 1021.633 kb on + strand, within AO356_04525 at 1021.633 kb on + strand, within AO356_04525 at 1021.634 kb on - strand, within AO356_04525 at 1021.634 kb on - strand, within AO356_04525 at 1021.634 kb on - strand, within AO356_04525 at 1021.634 kb on - strand, within AO356_04525 at 1021.634 kb on - strand, within AO356_04525 at 1021.634 kb on - strand, within AO356_04525 at 1021.637 kb on - strand, within AO356_04525 at 1021.637 kb on - strand, within AO356_04525 at 1021.637 kb on - strand, within AO356_04525 at 1021.637 kb on - strand, within AO356_04525 at 1021.637 kb on - strand, within AO356_04525 at 1021.637 kb on - strand, within AO356_04525 at 1021.958 kb on - strand, within AO356_04525 at 1021.958 kb on - strand, within AO356_04525 at 1022.284 kb on + strand, within AO356_04525 at 1022.284 kb on + strand, within AO356_04525 at 1022.284 kb on + strand, within AO356_04525 at 1022.285 kb on - strand, within AO356_04525 at 1022.285 kb on - strand, within AO356_04525 at 1022.405 kb on - strand at 1022.428 kb on + strand at 1022.428 kb on + strand at 1022.429 kb on - strand at 1022.474 kb on + strand at 1022.474 kb on + strand at 1022.475 kb on - strand at 1022.533 kb on - strand at 1022.533 kb on - strand at 1023.477 kb on + strand at 1023.477 kb on + strand at 1023.477 kb on + strand at 1023.477 kb on + strand at 1023.478 kb on - strand at 1023.478 kb on - strand at 1023.478 kb on - strand at 1023.478 kb on - strand at 1023.478 kb on - strand at 1023.478 kb on - strand at 1023.478 kb on - strand at 1023.478 kb on - strand at 1023.478 kb on - strand at 1023.505 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,020,624 + -0.8 1,020,624 + +1.4 1,020,625 - -0.2 1,020,625 - -1.7 1,020,625 - -0.7 1,020,625 - -0.4 1,020,625 - +0.9 1,020,625 - -0.4 1,020,625 - +0.1 1,020,629 - -1.9 1,020,629 - -0.2 1,020,874 - AO356_04520 0.31 -2.5 1,020,874 - AO356_04520 0.31 +0.1 1,020,880 - AO356_04520 0.32 -0.1 1,020,880 - AO356_04520 0.32 -2.1 1,020,880 - AO356_04520 0.32 -1.2 1,020,880 - AO356_04520 0.32 -0.2 1,020,970 - AO356_04520 0.44 +0.2 1,021,019 + AO356_04520 0.51 -0.4 1,021,020 - AO356_04520 0.51 +0.6 1,021,020 - AO356_04520 0.51 -0.0 1,021,020 - AO356_04520 0.51 +1.0 1,021,020 - AO356_04520 0.51 -2.3 1,021,020 - AO356_04520 0.51 -1.0 1,021,020 - AO356_04520 0.51 -1.0 1,021,020 - AO356_04520 0.51 -1.6 1,021,020 - AO356_04520 0.51 -0.5 1,021,020 - AO356_04520 0.51 +0.1 1,021,020 - AO356_04520 0.51 -1.8 1,021,024 - AO356_04520 0.51 +1.0 1,021,024 - AO356_04520 0.51 +0.5 1,021,024 - AO356_04520 0.51 -1.6 1,021,024 - AO356_04520 0.51 -0.9 1,021,024 - AO356_04520 0.51 -1.8 1,021,024 - AO356_04520 0.51 -2.4 1,021,024 - AO356_04520 0.51 -0.1 1,021,024 - AO356_04520 0.51 -0.6 1,021,024 - AO356_04520 0.51 +0.2 1,021,165 - AO356_04520 0.70 -0.1 1,021,165 - AO356_04520 0.70 +1.6 1,021,179 + AO356_04520 0.72 -0.5 1,021,179 + AO356_04520 0.72 +1.1 1,021,180 - AO356_04520 0.72 -0.3 1,021,326 + -1.9 1,021,326 + -0.8 1,021,326 + +0.7 1,021,326 + +1.4 1,021,326 + -0.6 1,021,327 - -1.6 1,021,327 - +1.1 1,021,327 - +2.8 1,021,327 - +2.1 1,021,327 - +0.1 1,021,327 - -2.4 1,021,330 + -1.8 1,021,330 + +0.1 1,021,330 + -0.1 1,021,331 - -2.7 1,021,331 - +0.5 1,021,331 - +1.1 1,021,331 - +3.4 1,021,331 - +1.9 1,021,331 - +1.9 1,021,428 + +0.2 1,021,428 + -1.4 1,021,429 - -2.4 1,021,429 - -1.1 1,021,429 - +2.1 1,021,633 + AO356_04525 0.21 +1.4 1,021,633 + AO356_04525 0.21 +0.2 1,021,633 + AO356_04525 0.21 -0.4 1,021,634 - AO356_04525 0.21 -1.5 1,021,634 - AO356_04525 0.21 +0.8 1,021,634 - AO356_04525 0.21 +1.0 1,021,634 - AO356_04525 0.21 +0.5 1,021,634 - AO356_04525 0.21 -1.5 1,021,634 - AO356_04525 0.21 +1.1 1,021,637 - AO356_04525 0.21 -0.1 1,021,637 - AO356_04525 0.21 +2.5 1,021,637 - AO356_04525 0.21 -1.0 1,021,637 - AO356_04525 0.21 -0.0 1,021,637 - AO356_04525 0.21 -0.3 1,021,637 - AO356_04525 0.21 -1.8 1,021,958 - AO356_04525 0.50 +0.7 1,021,958 - AO356_04525 0.50 -0.6 1,022,284 + AO356_04525 0.79 -1.3 1,022,284 + AO356_04525 0.79 -1.3 1,022,284 + AO356_04525 0.79 -0.2 1,022,285 - AO356_04525 0.79 -1.1 1,022,285 - AO356_04525 0.79 +0.3 1,022,405 - -1.1 1,022,428 + -0.8 1,022,428 + +1.9 1,022,429 - +1.7 1,022,474 + +0.1 1,022,474 + -0.9 1,022,475 - -1.2 1,022,533 - +0.1 1,022,533 - +0.6 1,023,477 + -0.8 1,023,477 + -1.7 1,023,477 + +0.6 1,023,477 + +2.0 1,023,478 - +0.2 1,023,478 - +0.5 1,023,478 - -1.4 1,023,478 - -0.9 1,023,478 - -0.7 1,023,478 - +1.0 1,023,478 - +0.7 1,023,478 - +0.4 1,023,478 - +0.5 1,023,505 + -0.8
Or see this region's nucleotide sequence