Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_00655 and AO356_00660 are separated by 348 nucleotides AO356_00660 and AO356_00665 are separated by 2 nucleotides AO356_00665 and AO356_00670 are separated by 21 nucleotides
AO356_00655: AO356_00655 - dioxygenase, at 140,079 to 141,527
_00655
AO356_00660: AO356_00660 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 141,876 to 142,727
_00660
AO356_00665: AO356_00665 - IclR family transcriptional regulator, at 142,730 to 143,551
_00665
AO356_00670: AO356_00670 - 3-(3-hydroxyphenyl)propionate hydroxylase, at 143,573 to 145,219
_00670
Position (kb) 141
142
143 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 140.930 kb on + strand, within AO356_00655 at 140.930 kb on + strand, within AO356_00655 at 140.930 kb on + strand, within AO356_00655 at 140.930 kb on + strand, within AO356_00655 at 140.931 kb on - strand, within AO356_00655 at 140.931 kb on - strand, within AO356_00655 at 140.931 kb on - strand, within AO356_00655 at 140.964 kb on + strand, within AO356_00655 at 140.964 kb on + strand, within AO356_00655 at 140.965 kb on - strand, within AO356_00655 at 140.965 kb on - strand, within AO356_00655 at 140.994 kb on - strand, within AO356_00655 at 141.001 kb on - strand, within AO356_00655 at 141.024 kb on + strand, within AO356_00655 at 141.024 kb on + strand, within AO356_00655 at 141.028 kb on + strand, within AO356_00655 at 141.029 kb on - strand, within AO356_00655 at 141.123 kb on + strand, within AO356_00655 at 141.213 kb on + strand, within AO356_00655 at 141.213 kb on + strand, within AO356_00655 at 141.214 kb on - strand, within AO356_00655 at 141.376 kb on - strand, within AO356_00655 at 141.607 kb on + strand at 141.608 kb on - strand at 141.615 kb on + strand at 141.615 kb on + strand at 141.616 kb on - strand at 141.616 kb on - strand at 141.751 kb on + strand at 141.751 kb on + strand at 141.751 kb on + strand at 141.752 kb on - strand at 141.752 kb on - strand at 141.834 kb on - strand at 141.876 kb on - strand at 141.915 kb on + strand at 141.915 kb on + strand at 142.122 kb on + strand, within AO356_00660 at 142.123 kb on - strand, within AO356_00660 at 142.179 kb on - strand, within AO356_00660 at 142.321 kb on - strand, within AO356_00660 at 142.321 kb on - strand, within AO356_00660 at 142.321 kb on - strand, within AO356_00660 at 142.342 kb on - strand, within AO356_00660 at 142.726 kb on - strand at 142.726 kb on - strand at 142.895 kb on + strand, within AO356_00665 at 142.902 kb on - strand, within AO356_00665 at 142.902 kb on - strand, within AO356_00665 at 143.330 kb on + strand, within AO356_00665 at 143.330 kb on + strand, within AO356_00665 at 143.568 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 140,930 + AO356_00655 0.59 -0.3 140,930 + AO356_00655 0.59 -1.6 140,930 + AO356_00655 0.59 +0.6 140,930 + AO356_00655 0.59 -0.0 140,931 - AO356_00655 0.59 -0.4 140,931 - AO356_00655 0.59 -1.5 140,931 - AO356_00655 0.59 +0.0 140,964 + AO356_00655 0.61 -0.1 140,964 + AO356_00655 0.61 -1.9 140,965 - AO356_00655 0.61 +0.6 140,965 - AO356_00655 0.61 +0.6 140,994 - AO356_00655 0.63 -2.4 141,001 - AO356_00655 0.64 +0.6 141,024 + AO356_00655 0.65 +0.1 141,024 + AO356_00655 0.65 -0.7 141,028 + AO356_00655 0.65 -0.6 141,029 - AO356_00655 0.66 -1.4 141,123 + AO356_00655 0.72 +0.1 141,213 + AO356_00655 0.78 -3.0 141,213 + AO356_00655 0.78 -0.7 141,214 - AO356_00655 0.78 -1.0 141,376 - AO356_00655 0.90 +0.2 141,607 + -0.6 141,608 - +0.4 141,615 + +0.9 141,615 + +0.7 141,616 - +2.5 141,616 - -0.0 141,751 + -1.4 141,751 + -2.3 141,751 + -1.6 141,752 - +2.3 141,752 - +2.1 141,834 - -0.0 141,876 - -1.4 141,915 + +0.4 141,915 + +0.8 142,122 + AO356_00660 0.29 -0.2 142,123 - AO356_00660 0.29 +0.8 142,179 - AO356_00660 0.36 -1.0 142,321 - AO356_00660 0.52 +0.6 142,321 - AO356_00660 0.52 -2.0 142,321 - AO356_00660 0.52 -0.4 142,342 - AO356_00660 0.55 +2.1 142,726 - +1.5 142,726 - -1.0 142,895 + AO356_00665 0.20 +0.9 142,902 - AO356_00665 0.21 +0.2 142,902 - AO356_00665 0.21 +0.4 143,330 + AO356_00665 0.73 +0.7 143,330 + AO356_00665 0.73 -2.2 143,568 + +1.0
Or see this region's nucleotide sequence