Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_25420 and AO353_25425 are separated by 15 nucleotides AO353_25425 and AO353_25430 overlap by 1 nucleotides AO353_25430 and AO353_25435 are separated by 27 nucleotides
AO353_25420: AO353_25420 - spermidine/putrescine ABC transporter ATP-binding protein, at 5,604,681 to 5,605,799
_25420
AO353_25425: AO353_25425 - ABC transporter permease, at 5,605,815 to 5,606,735
_25425
AO353_25430: AO353_25430 - ABC transporter permease, at 5,606,735 to 5,607,541
_25430
AO353_25435: AO353_25435 - spermidine/putrescine ABC transporter substrate-binding protein, at 5,607,569 to 5,608,615
_25435
Position (kb) 5606
5607
5608 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5605.853 kb on + strand at 5605.853 kb on + strand at 5605.853 kb on + strand at 5606.001 kb on + strand, within AO353_25425 at 5606.002 kb on - strand, within AO353_25425 at 5606.002 kb on - strand, within AO353_25425 at 5606.058 kb on + strand, within AO353_25425 at 5606.059 kb on - strand, within AO353_25425 at 5606.112 kb on - strand, within AO353_25425 at 5606.112 kb on - strand, within AO353_25425 at 5606.112 kb on - strand, within AO353_25425 at 5606.229 kb on + strand, within AO353_25425 at 5606.229 kb on + strand, within AO353_25425 at 5606.319 kb on + strand, within AO353_25425 at 5606.319 kb on + strand, within AO353_25425 at 5606.320 kb on - strand, within AO353_25425 at 5606.320 kb on - strand, within AO353_25425 at 5606.320 kb on - strand at 5606.320 kb on - strand, within AO353_25425 at 5606.320 kb on - strand, within AO353_25425 at 5606.320 kb on - strand, within AO353_25425 at 5606.320 kb on - strand, within AO353_25425 at 5606.320 kb on - strand, within AO353_25425 at 5606.352 kb on + strand, within AO353_25425 at 5606.353 kb on - strand, within AO353_25425 at 5606.353 kb on - strand, within AO353_25425 at 5606.353 kb on - strand, within AO353_25425 at 5606.385 kb on + strand, within AO353_25425 at 5606.439 kb on + strand, within AO353_25425 at 5606.439 kb on + strand, within AO353_25425 at 5606.688 kb on + strand at 5606.689 kb on - strand at 5606.689 kb on - strand at 5606.689 kb on - strand at 5606.689 kb on - strand at 5606.733 kb on + strand at 5606.733 kb on + strand at 5606.734 kb on - strand at 5606.734 kb on - strand at 5606.734 kb on - strand at 5606.745 kb on + strand at 5606.746 kb on - strand at 5606.746 kb on - strand at 5606.810 kb on + strand at 5606.811 kb on - strand at 5606.811 kb on - strand at 5606.816 kb on + strand, within AO353_25430 at 5606.816 kb on + strand, within AO353_25430 at 5606.817 kb on - strand, within AO353_25430 at 5606.817 kb on - strand at 5606.822 kb on + strand, within AO353_25430 at 5606.822 kb on + strand, within AO353_25430 at 5606.822 kb on + strand, within AO353_25430 at 5606.823 kb on - strand, within AO353_25430 at 5606.823 kb on - strand, within AO353_25430 at 5607.084 kb on + strand, within AO353_25430 at 5607.175 kb on + strand, within AO353_25430 at 5607.176 kb on - strand, within AO353_25430 at 5607.176 kb on - strand, within AO353_25430 at 5607.176 kb on - strand, within AO353_25430 at 5607.176 kb on - strand, within AO353_25430 at 5607.176 kb on - strand, within AO353_25430 at 5607.176 kb on - strand, within AO353_25430 at 5607.337 kb on - strand, within AO353_25430 at 5607.515 kb on + strand at 5607.515 kb on + strand at 5607.516 kb on - strand at 5607.516 kb on - strand at 5607.516 kb on - strand at 5607.564 kb on + strand at 5607.565 kb on - strand at 5607.565 kb on - strand at 5607.565 kb on - strand at 5607.565 kb on - strand at 5607.565 kb on - strand at 5607.568 kb on + strand at 5607.568 kb on + strand at 5607.568 kb on + strand at 5607.568 kb on + strand at 5607.569 kb on - strand at 5607.569 kb on - strand at 5607.569 kb on - strand at 5607.569 kb on - strand at 5607.569 kb on - strand at 5607.569 kb on - strand at 5607.569 kb on - strand at 5607.569 kb on - strand at 5607.680 kb on + strand, within AO353_25435 at 5607.680 kb on + strand, within AO353_25435 at 5607.681 kb on - strand, within AO353_25435 at 5607.746 kb on + strand, within AO353_25435 at 5607.746 kb on + strand, within AO353_25435 at 5607.747 kb on - strand, within AO353_25435 at 5607.885 kb on - strand, within AO353_25435 at 5607.887 kb on + strand, within AO353_25435 at 5607.888 kb on - strand, within AO353_25435 at 5607.920 kb on - strand, within AO353_25435 at 5607.920 kb on - strand, within AO353_25435 at 5607.995 kb on + strand, within AO353_25435 at 5607.996 kb on - strand, within AO353_25435 at 5607.997 kb on + strand, within AO353_25435 at 5607.997 kb on + strand, within AO353_25435 at 5607.997 kb on + strand, within AO353_25435 at 5607.997 kb on + strand, within AO353_25435 at 5607.997 kb on + strand, within AO353_25435 at 5607.997 kb on + strand, within AO353_25435 at 5607.998 kb on - strand, within AO353_25435 at 5607.998 kb on - strand, within AO353_25435 at 5608.016 kb on + strand, within AO353_25435 at 5608.016 kb on + strand, within AO353_25435 at 5608.016 kb on + strand, within AO353_25435 at 5608.016 kb on + strand, within AO353_25435 at 5608.016 kb on + strand, within AO353_25435 at 5608.016 kb on + strand, within AO353_25435 at 5608.016 kb on + strand, within AO353_25435 at 5608.016 kb on + strand, within AO353_25435 at 5608.016 kb on + strand, within AO353_25435 at 5608.016 kb on + strand, within AO353_25435 at 5608.017 kb on - strand, within AO353_25435 at 5608.017 kb on - strand, within AO353_25435 at 5608.017 kb on - strand, within AO353_25435 at 5608.017 kb on - strand, within AO353_25435 at 5608.028 kb on + strand, within AO353_25435 at 5608.028 kb on + strand, within AO353_25435 at 5608.063 kb on + strand, within AO353_25435 at 5608.063 kb on + strand, within AO353_25435 at 5608.063 kb on + strand, within AO353_25435 at 5608.063 kb on + strand, within AO353_25435 at 5608.064 kb on - strand, within AO353_25435 at 5608.064 kb on - strand, within AO353_25435 at 5608.064 kb on - strand, within AO353_25435 at 5608.064 kb on - strand, within AO353_25435 at 5608.064 kb on - strand, within AO353_25435 at 5608.064 kb on - strand, within AO353_25435 at 5608.064 kb on - strand, within AO353_25435 at 5608.106 kb on + strand, within AO353_25435 at 5608.106 kb on + strand, within AO353_25435 at 5608.106 kb on + strand, within AO353_25435 at 5608.160 kb on - strand, within AO353_25435 at 5608.197 kb on - strand, within AO353_25435 at 5608.310 kb on + strand, within AO353_25435 at 5608.310 kb on + strand at 5608.310 kb on + strand, within AO353_25435 at 5608.310 kb on + strand, within AO353_25435 at 5608.310 kb on + strand, within AO353_25435 at 5608.311 kb on - strand, within AO353_25435 at 5608.311 kb on - strand, within AO353_25435 at 5608.311 kb on - strand, within AO353_25435 at 5608.311 kb on - strand, within AO353_25435 at 5608.311 kb on - strand, within AO353_25435 at 5608.311 kb on - strand, within AO353_25435 at 5608.311 kb on - strand, within AO353_25435
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 5,605,853 + +1.0 5,605,853 + -0.1 5,605,853 + -2.6 5,606,001 + AO353_25425 0.20 -0.5 5,606,002 - AO353_25425 0.20 -0.2 5,606,002 - AO353_25425 0.20 +2.0 5,606,058 + AO353_25425 0.26 -1.9 5,606,059 - AO353_25425 0.26 +1.1 5,606,112 - AO353_25425 0.32 +2.0 5,606,112 - AO353_25425 0.32 -0.2 5,606,112 - AO353_25425 0.32 +0.8 5,606,229 + AO353_25425 0.45 -1.6 5,606,229 + AO353_25425 0.45 -2.6 5,606,319 + AO353_25425 0.55 +1.1 5,606,319 + AO353_25425 0.55 -1.1 5,606,320 - AO353_25425 0.55 +2.5 5,606,320 - AO353_25425 0.55 +1.8 5,606,320 - +2.8 5,606,320 - AO353_25425 0.55 -1.1 5,606,320 - AO353_25425 0.55 +2.4 5,606,320 - AO353_25425 0.55 -2.2 5,606,320 - AO353_25425 0.55 -1.6 5,606,320 - AO353_25425 0.55 -1.3 5,606,352 + AO353_25425 0.58 +1.9 5,606,353 - AO353_25425 0.58 -1.3 5,606,353 - AO353_25425 0.58 -1.6 5,606,353 - AO353_25425 0.58 +0.4 5,606,385 + AO353_25425 0.62 -1.6 5,606,439 + AO353_25425 0.68 -1.4 5,606,439 + AO353_25425 0.68 +0.3 5,606,688 + +1.5 5,606,689 - -2.8 5,606,689 - -1.8 5,606,689 - +0.5 5,606,689 - +1.0 5,606,733 + -1.1 5,606,733 + +0.9 5,606,734 - -0.8 5,606,734 - -1.9 5,606,734 - +0.7 5,606,745 + -1.7 5,606,746 - +0.8 5,606,746 - +0.3 5,606,810 + -0.8 5,606,811 - -0.1 5,606,811 - +0.2 5,606,816 + AO353_25430 0.10 +1.3 5,606,816 + AO353_25430 0.10 -1.9 5,606,817 - AO353_25430 0.10 -1.5 5,606,817 - -0.1 5,606,822 + AO353_25430 0.11 -1.6 5,606,822 + AO353_25430 0.11 -1.9 5,606,822 + AO353_25430 0.11 +1.2 5,606,823 - AO353_25430 0.11 +1.1 5,606,823 - AO353_25430 0.11 +1.1 5,607,084 + AO353_25430 0.43 -1.8 5,607,175 + AO353_25430 0.55 -0.8 5,607,176 - AO353_25430 0.55 +1.0 5,607,176 - AO353_25430 0.55 -1.1 5,607,176 - AO353_25430 0.55 -0.7 5,607,176 - AO353_25430 0.55 +1.1 5,607,176 - AO353_25430 0.55 +1.0 5,607,176 - AO353_25430 0.55 -1.7 5,607,337 - AO353_25430 0.75 -1.0 5,607,515 + -2.2 5,607,515 + +1.4 5,607,516 - -1.5 5,607,516 - +1.2 5,607,516 - +1.1 5,607,564 + +1.6 5,607,565 - -0.3 5,607,565 - +1.2 5,607,565 - -0.6 5,607,565 - +2.1 5,607,565 - -1.5 5,607,568 + -0.1 5,607,568 + -0.8 5,607,568 + +1.5 5,607,568 + +1.3 5,607,569 - -1.1 5,607,569 - -0.7 5,607,569 - +1.0 5,607,569 - +1.7 5,607,569 - +2.2 5,607,569 - +1.5 5,607,569 - +0.4 5,607,569 - +0.5 5,607,680 + AO353_25435 0.11 +1.3 5,607,680 + AO353_25435 0.11 +0.8 5,607,681 - AO353_25435 0.11 +0.3 5,607,746 + AO353_25435 0.17 -0.5 5,607,746 + AO353_25435 0.17 +0.7 5,607,747 - AO353_25435 0.17 -1.2 5,607,885 - AO353_25435 0.30 -0.8 5,607,887 + AO353_25435 0.30 -1.3 5,607,888 - AO353_25435 0.30 -0.8 5,607,920 - AO353_25435 0.34 -0.8 5,607,920 - AO353_25435 0.34 +2.3 5,607,995 + AO353_25435 0.41 -0.4 5,607,996 - AO353_25435 0.41 +0.8 5,607,997 + AO353_25435 0.41 -0.1 5,607,997 + AO353_25435 0.41 -1.0 5,607,997 + AO353_25435 0.41 -1.1 5,607,997 + AO353_25435 0.41 +0.7 5,607,997 + AO353_25435 0.41 -1.6 5,607,997 + AO353_25435 0.41 +0.3 5,607,998 - AO353_25435 0.41 -0.2 5,607,998 - AO353_25435 0.41 +0.5 5,608,016 + AO353_25435 0.43 +0.5 5,608,016 + AO353_25435 0.43 +0.4 5,608,016 + AO353_25435 0.43 +2.1 5,608,016 + AO353_25435 0.43 +0.0 5,608,016 + AO353_25435 0.43 +0.8 5,608,016 + AO353_25435 0.43 +0.4 5,608,016 + AO353_25435 0.43 +0.2 5,608,016 + AO353_25435 0.43 +1.5 5,608,016 + AO353_25435 0.43 -0.3 5,608,016 + AO353_25435 0.43 -0.1 5,608,017 - AO353_25435 0.43 +0.3 5,608,017 - AO353_25435 0.43 +0.3 5,608,017 - AO353_25435 0.43 +0.1 5,608,017 - AO353_25435 0.43 -0.8 5,608,028 + AO353_25435 0.44 -1.5 5,608,028 + AO353_25435 0.44 -0.7 5,608,063 + AO353_25435 0.47 +0.7 5,608,063 + AO353_25435 0.47 -1.6 5,608,063 + AO353_25435 0.47 +1.3 5,608,063 + AO353_25435 0.47 -1.3 5,608,064 - AO353_25435 0.47 +1.0 5,608,064 - AO353_25435 0.47 +0.4 5,608,064 - AO353_25435 0.47 -1.0 5,608,064 - AO353_25435 0.47 -0.2 5,608,064 - AO353_25435 0.47 +2.1 5,608,064 - AO353_25435 0.47 -1.3 5,608,064 - AO353_25435 0.47 +2.3 5,608,106 + AO353_25435 0.51 +0.2 5,608,106 + AO353_25435 0.51 +1.2 5,608,106 + AO353_25435 0.51 -0.2 5,608,160 - AO353_25435 0.56 -3.2 5,608,197 - AO353_25435 0.60 -0.8 5,608,310 + AO353_25435 0.71 -1.3 5,608,310 + +0.9 5,608,310 + AO353_25435 0.71 -0.7 5,608,310 + AO353_25435 0.71 +0.8 5,608,310 + AO353_25435 0.71 -2.3 5,608,311 - AO353_25435 0.71 -0.6 5,608,311 - AO353_25435 0.71 +0.3 5,608,311 - AO353_25435 0.71 +0.4 5,608,311 - AO353_25435 0.71 +0.7 5,608,311 - AO353_25435 0.71 -0.8 5,608,311 - AO353_25435 0.71 -0.6 5,608,311 - AO353_25435 0.71 +0.4
Or see this region's nucleotide sequence