Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_27430 and AO356_27435 are separated by 22 nucleotides AO356_27435 and AO356_27440 are separated by 3 nucleotides AO356_27440 and AO356_27445 overlap by 4 nucleotides
AO356_27430: AO356_27430 - hypothetical protein, at 6,251,109 to 6,251,804
_27430
AO356_27435: AO356_27435 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 6,251,827 to 6,252,720
_27435
AO356_27440: AO356_27440 - 2-keto-4-pentenoate hydratase, at 6,252,724 to 6,253,524
_27440
AO356_27445: AO356_27445 - IclR family transcriptional regulator, at 6,253,521 to 6,254,327
_27445
Position (kb)
6252
6253
6254 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6251.734 kb on + strand, within AO356_27430 at 6251.734 kb on + strand, within AO356_27430 at 6251.782 kb on - strand at 6251.782 kb on - strand at 6251.804 kb on + strand at 6251.882 kb on + strand at 6251.882 kb on + strand at 6251.882 kb on + strand at 6251.882 kb on + strand at 6251.883 kb on - strand at 6251.883 kb on - strand at 6251.883 kb on - strand at 6251.883 kb on - strand at 6252.045 kb on - strand, within AO356_27435 at 6252.045 kb on - strand, within AO356_27435 at 6252.055 kb on - strand, within AO356_27435 at 6252.084 kb on + strand, within AO356_27435 at 6252.228 kb on - strand, within AO356_27435 at 6252.456 kb on + strand, within AO356_27435 at 6252.456 kb on + strand, within AO356_27435 at 6252.457 kb on - strand, within AO356_27435 at 6252.483 kb on - strand, within AO356_27435 at 6252.483 kb on - strand, within AO356_27435 at 6252.520 kb on + strand, within AO356_27435 at 6252.520 kb on + strand, within AO356_27435 at 6252.521 kb on - strand, within AO356_27435 at 6252.521 kb on - strand, within AO356_27435 at 6252.712 kb on + strand at 6252.713 kb on - strand at 6252.726 kb on - strand at 6252.821 kb on + strand, within AO356_27440 at 6252.821 kb on + strand, within AO356_27440 at 6252.821 kb on + strand, within AO356_27440 at 6252.822 kb on - strand, within AO356_27440 at 6252.822 kb on - strand, within AO356_27440 at 6253.045 kb on + strand, within AO356_27440 at 6253.045 kb on + strand, within AO356_27440 at 6253.045 kb on + strand, within AO356_27440 at 6253.046 kb on - strand, within AO356_27440 at 6253.092 kb on + strand, within AO356_27440 at 6253.092 kb on + strand, within AO356_27440 at 6253.092 kb on + strand, within AO356_27440 at 6253.093 kb on - strand, within AO356_27440 at 6253.093 kb on - strand, within AO356_27440 at 6253.093 kb on - strand, within AO356_27440 at 6253.161 kb on + strand, within AO356_27440 at 6253.162 kb on - strand, within AO356_27440 at 6253.162 kb on - strand, within AO356_27440 at 6253.163 kb on + strand, within AO356_27440 at 6253.163 kb on + strand, within AO356_27440 at 6253.164 kb on - strand, within AO356_27440 at 6253.164 kb on - strand, within AO356_27440 at 6253.164 kb on - strand, within AO356_27440 at 6253.164 kb on - strand, within AO356_27440 at 6253.295 kb on - strand, within AO356_27440 at 6253.524 kb on + strand at 6253.524 kb on + strand at 6253.524 kb on + strand at 6253.525 kb on - strand at 6253.525 kb on - strand at 6253.525 kb on - strand at 6253.613 kb on - strand, within AO356_27445 at 6253.613 kb on - strand, within AO356_27445 at 6253.919 kb on - strand, within AO356_27445 at 6253.952 kb on - strand, within AO356_27445 at 6253.956 kb on - strand, within AO356_27445 at 6253.956 kb on - strand, within AO356_27445 at 6253.956 kb on - strand, within AO356_27445 at 6254.098 kb on - strand, within AO356_27445 at 6254.098 kb on - strand, within AO356_27445 at 6254.098 kb on - strand, within AO356_27445 at 6254.098 kb on - strand, within AO356_27445 at 6254.098 kb on - strand, within AO356_27445 at 6254.098 kb on - strand, within AO356_27445 at 6254.510 kb on + strand at 6254.510 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 6,251,734 + AO356_27430 0.90 -0.5 6,251,734 + AO356_27430 0.90 -0.9 6,251,782 - +0.7 6,251,782 - -0.8 6,251,804 + -0.7 6,251,882 + -0.7 6,251,882 + +1.8 6,251,882 + +1.5 6,251,882 + +0.2 6,251,883 - -1.1 6,251,883 - -2.8 6,251,883 - +0.6 6,251,883 - +0.9 6,252,045 - AO356_27435 0.24 +1.0 6,252,045 - AO356_27435 0.24 +1.1 6,252,055 - AO356_27435 0.26 -2.7 6,252,084 + AO356_27435 0.29 +0.5 6,252,228 - AO356_27435 0.45 +0.2 6,252,456 + AO356_27435 0.70 +1.2 6,252,456 + AO356_27435 0.70 +0.6 6,252,457 - AO356_27435 0.70 +0.9 6,252,483 - AO356_27435 0.73 -1.4 6,252,483 - AO356_27435 0.73 -1.1 6,252,520 + AO356_27435 0.78 +1.0 6,252,520 + AO356_27435 0.78 -0.6 6,252,521 - AO356_27435 0.78 +2.4 6,252,521 - AO356_27435 0.78 -0.1 6,252,712 + -1.5 6,252,713 - +1.6 6,252,726 - +0.2 6,252,821 + AO356_27440 0.12 -1.5 6,252,821 + AO356_27440 0.12 -0.8 6,252,821 + AO356_27440 0.12 -2.8 6,252,822 - AO356_27440 0.12 -0.9 6,252,822 - AO356_27440 0.12 +0.3 6,253,045 + AO356_27440 0.40 +1.0 6,253,045 + AO356_27440 0.40 +1.1 6,253,045 + AO356_27440 0.40 -1.5 6,253,046 - AO356_27440 0.40 -0.4 6,253,092 + AO356_27440 0.46 -0.1 6,253,092 + AO356_27440 0.46 -1.3 6,253,092 + AO356_27440 0.46 -1.4 6,253,093 - AO356_27440 0.46 +2.8 6,253,093 - AO356_27440 0.46 -1.1 6,253,093 - AO356_27440 0.46 -1.7 6,253,161 + AO356_27440 0.55 +0.8 6,253,162 - AO356_27440 0.55 +0.1 6,253,162 - AO356_27440 0.55 +0.6 6,253,163 + AO356_27440 0.55 +1.4 6,253,163 + AO356_27440 0.55 +0.7 6,253,164 - AO356_27440 0.55 -1.5 6,253,164 - AO356_27440 0.55 -1.1 6,253,164 - AO356_27440 0.55 -0.1 6,253,164 - AO356_27440 0.55 -1.1 6,253,295 - AO356_27440 0.71 -0.4 6,253,524 + -0.4 6,253,524 + -0.6 6,253,524 + +1.3 6,253,525 - +1.9 6,253,525 - -1.1 6,253,525 - -3.3 6,253,613 - AO356_27445 0.11 -1.1 6,253,613 - AO356_27445 0.11 -0.3 6,253,919 - AO356_27445 0.49 +2.5 6,253,952 - AO356_27445 0.53 +0.2 6,253,956 - AO356_27445 0.54 -1.1 6,253,956 - AO356_27445 0.54 +0.3 6,253,956 - AO356_27445 0.54 -2.3 6,254,098 - AO356_27445 0.71 -1.9 6,254,098 - AO356_27445 0.71 -2.7 6,254,098 - AO356_27445 0.71 -0.4 6,254,098 - AO356_27445 0.71 -2.0 6,254,098 - AO356_27445 0.71 -0.5 6,254,098 - AO356_27445 0.71 +0.3 6,254,510 + -1.1 6,254,510 + -1.7
Or see this region's nucleotide sequence