Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_25215 and AO356_25220 overlap by 8 nucleotides AO356_25220 and AO356_25225 are separated by 10 nucleotides AO356_25225 and AO356_25230 are separated by 25 nucleotides
AO356_25215: AO356_25215 - nitrate reductase, at 5,708,501 to 5,709,256
_25215
AO356_25220: AO356_25220 - nitrate reductase, at 5,709,249 to 5,709,929
_25220
AO356_25225: AO356_25225 - peptidylprolyl isomerase, at 5,709,940 to 5,710,893
_25225
AO356_25230: AO356_25230 - cyclic pyranopterin phosphate synthase MoaA, at 5,710,919 to 5,711,911
_25230
Position (kb) 5709
5710
5711 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5708.960 kb on + strand, within AO356_25215 at 5709.282 kb on + strand at 5709.282 kb on + strand at 5709.288 kb on + strand at 5709.288 kb on + strand at 5709.289 kb on - strand at 5709.345 kb on + strand, within AO356_25220 at 5709.345 kb on + strand, within AO356_25220 at 5709.345 kb on + strand, within AO356_25220 at 5709.478 kb on - strand, within AO356_25220 at 5709.478 kb on - strand, within AO356_25220 at 5710.694 kb on - strand, within AO356_25225 at 5710.816 kb on + strand at 5710.817 kb on - strand at 5711.007 kb on - strand at 5711.007 kb on - strand at 5711.160 kb on + strand, within AO356_25230 at 5711.161 kb on - strand, within AO356_25230 at 5711.450 kb on + strand, within AO356_25230 at 5711.450 kb on + strand, within AO356_25230 at 5711.618 kb on + strand, within AO356_25230 at 5711.619 kb on - strand, within AO356_25230 at 5711.677 kb on + strand, within AO356_25230
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,708,960 + AO356_25215 0.61 -0.7 5,709,282 + +0.3 5,709,282 + +0.9 5,709,288 + +0.6 5,709,288 + +0.9 5,709,289 - +1.0 5,709,345 + AO356_25220 0.14 +2.6 5,709,345 + AO356_25220 0.14 +0.5 5,709,345 + AO356_25220 0.14 +0.9 5,709,478 - AO356_25220 0.34 +1.5 5,709,478 - AO356_25220 0.34 -1.1 5,710,694 - AO356_25225 0.79 -3.3 5,710,816 + +0.7 5,710,817 - -3.3 5,711,007 - -2.0 5,711,007 - -1.2 5,711,160 + AO356_25230 0.24 -2.6 5,711,161 - AO356_25230 0.24 -1.4 5,711,450 + AO356_25230 0.53 +1.4 5,711,450 + AO356_25230 0.53 -0.1 5,711,618 + AO356_25230 0.70 -2.3 5,711,619 - AO356_25230 0.70 -2.1 5,711,677 + AO356_25230 0.76 +0.1
Or see this region's nucleotide sequence