Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_24730 and AO356_24735 are separated by 205 nucleotides AO356_24735 and AO356_24740 overlap by 4 nucleotides AO356_24740 and AO356_24745 are separated by 37 nucleotides
AO356_24730: AO356_24730 - hypothetical protein, at 5,595,635 to 5,595,961
_24730
AO356_24735: AO356_24735 - TonB-dependent outer membrane receptor, at 5,596,167 to 5,596,799
_24735
AO356_24740: AO356_24740 - RNA polymerase subunit sigma-24, at 5,596,796 to 5,597,341
_24740
AO356_24745: AO356_24745 - iron dicitrate transport regulator FecR, at 5,597,379 to 5,598,320
_24745
Position (kb) 5596
5597
5598 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5595.841 kb on - strand, within AO356_24730 at 5595.856 kb on - strand, within AO356_24730 at 5596.014 kb on + strand at 5596.015 kb on - strand at 5596.036 kb on - strand at 5596.401 kb on + strand, within AO356_24735 at 5596.402 kb on - strand, within AO356_24735 at 5596.494 kb on + strand, within AO356_24735 at 5596.531 kb on + strand, within AO356_24735 at 5596.531 kb on + strand, within AO356_24735 at 5596.531 kb on + strand, within AO356_24735 at 5596.532 kb on - strand, within AO356_24735 at 5596.844 kb on + strand at 5596.845 kb on - strand at 5596.920 kb on - strand, within AO356_24740 at 5596.984 kb on + strand, within AO356_24740 at 5597.141 kb on + strand, within AO356_24740 at 5597.142 kb on - strand, within AO356_24740 at 5597.346 kb on - strand at 5597.712 kb on + strand, within AO356_24745 at 5597.712 kb on + strand, within AO356_24745 at 5598.036 kb on + strand, within AO356_24745 at 5598.036 kb on + strand, within AO356_24745
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,595,841 - AO356_24730 0.63 -0.4 5,595,856 - AO356_24730 0.68 -0.2 5,596,014 + +0.3 5,596,015 - -0.4 5,596,036 - -2.0 5,596,401 + AO356_24735 0.37 +0.3 5,596,402 - AO356_24735 0.37 +0.7 5,596,494 + AO356_24735 0.52 -0.2 5,596,531 + AO356_24735 0.58 +1.6 5,596,531 + AO356_24735 0.58 -1.0 5,596,531 + AO356_24735 0.58 +1.7 5,596,532 - AO356_24735 0.58 +0.6 5,596,844 + +0.4 5,596,845 - +0.3 5,596,920 - AO356_24740 0.23 +2.6 5,596,984 + AO356_24740 0.34 -1.7 5,597,141 + AO356_24740 0.63 -1.4 5,597,142 - AO356_24740 0.63 -1.2 5,597,346 - -1.6 5,597,712 + AO356_24745 0.35 +1.4 5,597,712 + AO356_24745 0.35 -1.4 5,598,036 + AO356_24745 0.70 +0.6 5,598,036 + AO356_24745 0.70 +3.7
Or see this region's nucleotide sequence