Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_24380 and AO356_24385 are separated by 458 nucleotides AO356_24385 and AO356_24390 are separated by 334 nucleotides
AO356_24380: AO356_24380 - hydroxyacid dehydrogenase, at 5,504,571 to 5,505,524
_24380
AO356_24385: AO356_24385 - serine/threonine protein kinase, at 5,505,983 to 5,507,263
_24385
AO356_24390: AO356_24390 - bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase, at 5,507,598 to 5,510,207
_24390
Position (kb)
5505
5506
5507
5508 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5504.984 kb on - strand, within AO356_24380 at 5505.016 kb on + strand, within AO356_24380 at 5505.481 kb on + strand at 5505.482 kb on - strand at 5505.575 kb on - strand at 5505.672 kb on + strand at 5505.857 kb on - strand at 5506.119 kb on + strand, within AO356_24385 at 5506.193 kb on + strand, within AO356_24385 at 5506.193 kb on + strand, within AO356_24385 at 5506.193 kb on + strand, within AO356_24385 at 5506.193 kb on + strand, within AO356_24385 at 5506.194 kb on - strand, within AO356_24385 at 5506.194 kb on - strand, within AO356_24385 at 5506.194 kb on - strand, within AO356_24385 at 5506.308 kb on - strand, within AO356_24385 at 5506.341 kb on - strand, within AO356_24385 at 5506.503 kb on - strand, within AO356_24385 at 5506.503 kb on - strand, within AO356_24385 at 5506.503 kb on - strand, within AO356_24385 at 5506.564 kb on + strand, within AO356_24385 at 5506.565 kb on - strand, within AO356_24385 at 5506.612 kb on + strand, within AO356_24385 at 5506.612 kb on + strand, within AO356_24385 at 5506.612 kb on + strand, within AO356_24385 at 5506.613 kb on - strand, within AO356_24385 at 5506.613 kb on - strand, within AO356_24385 at 5506.613 kb on - strand, within AO356_24385 at 5506.613 kb on - strand, within AO356_24385 at 5506.613 kb on - strand, within AO356_24385 at 5506.857 kb on - strand, within AO356_24385 at 5506.946 kb on + strand, within AO356_24385 at 5506.946 kb on + strand, within AO356_24385 at 5506.947 kb on - strand, within AO356_24385 at 5506.947 kb on - strand, within AO356_24385 at 5507.147 kb on + strand at 5507.147 kb on + strand at 5507.147 kb on + strand at 5507.147 kb on + strand at 5507.147 kb on + strand at 5507.148 kb on - strand at 5507.467 kb on + strand at 5507.467 kb on + strand at 5507.541 kb on + strand at 5507.542 kb on - strand at 5507.593 kb on - strand at 5507.599 kb on + strand at 5507.680 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,504,984 - AO356_24380 0.43 -0.7 5,505,016 + AO356_24380 0.47 -0.0 5,505,481 + +1.5 5,505,482 - +0.9 5,505,575 - -0.8 5,505,672 + +0.4 5,505,857 - -0.2 5,506,119 + AO356_24385 0.11 +0.5 5,506,193 + AO356_24385 0.16 +0.7 5,506,193 + AO356_24385 0.16 +1.4 5,506,193 + AO356_24385 0.16 -0.2 5,506,193 + AO356_24385 0.16 +0.7 5,506,194 - AO356_24385 0.16 +1.9 5,506,194 - AO356_24385 0.16 -0.2 5,506,194 - AO356_24385 0.16 -0.5 5,506,308 - AO356_24385 0.25 +0.4 5,506,341 - AO356_24385 0.28 +0.3 5,506,503 - AO356_24385 0.41 +1.2 5,506,503 - AO356_24385 0.41 -1.5 5,506,503 - AO356_24385 0.41 +1.4 5,506,564 + AO356_24385 0.45 -0.3 5,506,565 - AO356_24385 0.45 +0.4 5,506,612 + AO356_24385 0.49 -2.2 5,506,612 + AO356_24385 0.49 -0.0 5,506,612 + AO356_24385 0.49 -2.6 5,506,613 - AO356_24385 0.49 -1.3 5,506,613 - AO356_24385 0.49 -2.0 5,506,613 - AO356_24385 0.49 +2.8 5,506,613 - AO356_24385 0.49 -0.1 5,506,613 - AO356_24385 0.49 +2.3 5,506,857 - AO356_24385 0.68 -1.8 5,506,946 + AO356_24385 0.75 +2.1 5,506,946 + AO356_24385 0.75 -1.3 5,506,947 - AO356_24385 0.75 -2.2 5,506,947 - AO356_24385 0.75 -0.8 5,507,147 + -1.2 5,507,147 + -0.6 5,507,147 + +0.8 5,507,147 + -1.0 5,507,147 + +0.9 5,507,148 - -0.6 5,507,467 + -2.6 5,507,467 + -2.0 5,507,541 + -0.8 5,507,542 - -2.4 5,507,593 - +1.4 5,507,599 + -1.8 5,507,680 - -1.4
Or see this region's nucleotide sequence