Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_23975 and AO356_23980 are separated by 257 nucleotides AO356_23980 and AO356_23985 are separated by 36 nucleotides AO356_23985 and AO356_23990 are separated by 242 nucleotides
AO356_23975: AO356_23975 - chemotaxis protein CheY, at 5,376,622 to 5,377,023
_23975
AO356_23980: AO356_23980 - HIT family hydrolase, at 5,377,281 to 5,377,724
_23980
AO356_23985: AO356_23985 - LysR family transcriptional regulator, at 5,377,761 to 5,378,618
_23985
AO356_23990: AO356_23990 - amino acid ABC transporter, at 5,378,861 to 5,379,703
_23990
Position (kb) 5377
5378
5379 Strain fitness (log2 ratio) -1
0
1
2 at 5377.125 kb on - strand at 5377.148 kb on - strand at 5377.205 kb on + strand at 5377.231 kb on + strand at 5377.232 kb on - strand at 5377.232 kb on - strand at 5377.300 kb on - strand at 5377.300 kb on - strand at 5377.300 kb on - strand at 5377.359 kb on + strand, within AO356_23980 at 5377.499 kb on - strand, within AO356_23980 at 5377.799 kb on - strand at 5377.849 kb on + strand, within AO356_23985 at 5377.849 kb on + strand, within AO356_23985 at 5377.849 kb on + strand, within AO356_23985 at 5377.850 kb on - strand, within AO356_23985 at 5377.850 kb on - strand, within AO356_23985 at 5377.850 kb on - strand, within AO356_23985 at 5377.923 kb on - strand, within AO356_23985 at 5377.990 kb on + strand, within AO356_23985 at 5377.991 kb on - strand, within AO356_23985 at 5378.047 kb on + strand, within AO356_23985 at 5378.047 kb on + strand, within AO356_23985 at 5378.105 kb on - strand, within AO356_23985 at 5378.884 kb on - strand at 5379.338 kb on + strand, within AO356_23990 at 5379.339 kb on - strand, within AO356_23990 at 5379.339 kb on - strand, within AO356_23990
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,377,125 - -0.0 5,377,148 - +0.2 5,377,205 + +1.3 5,377,231 + -0.3 5,377,232 - +1.3 5,377,232 - +1.0 5,377,300 - -1.2 5,377,300 - +1.1 5,377,300 - +0.3 5,377,359 + AO356_23980 0.18 +0.0 5,377,499 - AO356_23980 0.49 -0.9 5,377,799 - -0.2 5,377,849 + AO356_23985 0.10 +0.8 5,377,849 + AO356_23985 0.10 -1.3 5,377,849 + AO356_23985 0.10 -1.4 5,377,850 - AO356_23985 0.10 -1.4 5,377,850 - AO356_23985 0.10 +0.2 5,377,850 - AO356_23985 0.10 +0.7 5,377,923 - AO356_23985 0.19 +0.9 5,377,990 + AO356_23985 0.27 +0.9 5,377,991 - AO356_23985 0.27 -0.2 5,378,047 + AO356_23985 0.33 -1.4 5,378,047 + AO356_23985 0.33 -0.0 5,378,105 - AO356_23985 0.40 +0.5 5,378,884 - +0.2 5,379,338 + AO356_23990 0.57 -0.2 5,379,339 - AO356_23990 0.57 +1.8 5,379,339 - AO356_23990 0.57 +1.6
Or see this region's nucleotide sequence