Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_23595 and AO356_23600 are separated by 60 nucleotides AO356_23600 and AO356_23605 are separated by 32 nucleotides
AO356_23595: AO356_23595 - short-chain dehydrogenase, at 5,294,944 to 5,295,708
_23595
AO356_23600: AO356_23600 - hypothetical protein, at 5,295,769 to 5,296,824
_23600
AO356_23605: AO356_23605 - hypothetical protein, at 5,296,857 to 5,298,674
_23605
Position (kb) 5295
5296
5297 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5295.066 kb on - strand, within AO356_23595 at 5295.374 kb on - strand, within AO356_23595 at 5295.726 kb on - strand at 5296.253 kb on + strand, within AO356_23600 at 5296.253 kb on + strand, within AO356_23600 at 5296.398 kb on - strand, within AO356_23600 at 5296.485 kb on - strand, within AO356_23600 at 5296.570 kb on + strand, within AO356_23600 at 5296.571 kb on - strand, within AO356_23600 at 5296.571 kb on - strand, within AO356_23600 at 5296.571 kb on - strand, within AO356_23600 at 5296.824 kb on + strand at 5296.824 kb on + strand at 5296.824 kb on + strand at 5296.825 kb on - strand at 5296.825 kb on - strand at 5296.825 kb on - strand at 5296.825 kb on - strand at 5296.825 kb on - strand at 5296.891 kb on + strand at 5296.963 kb on + strand at 5296.964 kb on - strand at 5297.033 kb on + strand at 5297.033 kb on + strand at 5297.035 kb on + strand at 5297.036 kb on - strand at 5297.036 kb on - strand at 5297.063 kb on - strand, within AO356_23605 at 5297.165 kb on - strand, within AO356_23605 at 5297.165 kb on - strand, within AO356_23605 at 5297.437 kb on + strand, within AO356_23605 at 5297.450 kb on - strand, within AO356_23605 at 5297.563 kb on - strand, within AO356_23605 at 5297.602 kb on + strand, within AO356_23605 at 5297.602 kb on + strand, within AO356_23605 at 5297.608 kb on + strand, within AO356_23605 at 5297.608 kb on + strand, within AO356_23605 at 5297.608 kb on + strand, within AO356_23605 at 5297.608 kb on + strand, within AO356_23605 at 5297.609 kb on - strand, within AO356_23605 at 5297.609 kb on - strand, within AO356_23605 at 5297.609 kb on - strand, within AO356_23605 at 5297.609 kb on - strand, within AO356_23605 at 5297.609 kb on - strand, within AO356_23605 at 5297.609 kb on - strand, within AO356_23605 at 5297.609 kb on - strand, within AO356_23605 at 5297.609 kb on - strand, within AO356_23605 at 5297.609 kb on - strand, within AO356_23605 at 5297.611 kb on + strand, within AO356_23605 at 5297.611 kb on + strand, within AO356_23605 at 5297.686 kb on + strand, within AO356_23605 at 5297.687 kb on - strand, within AO356_23605 at 5297.687 kb on - strand, within AO356_23605 at 5297.803 kb on + strand, within AO356_23605
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,295,066 - AO356_23595 0.16 +0.4 5,295,374 - AO356_23595 0.56 -1.8 5,295,726 - -0.3 5,296,253 + AO356_23600 0.46 -2.0 5,296,253 + AO356_23600 0.46 -0.6 5,296,398 - AO356_23600 0.60 +0.2 5,296,485 - AO356_23600 0.68 -0.2 5,296,570 + AO356_23600 0.76 -0.9 5,296,571 - AO356_23600 0.76 -0.7 5,296,571 - AO356_23600 0.76 +0.7 5,296,571 - AO356_23600 0.76 +0.9 5,296,824 + +1.2 5,296,824 + -0.4 5,296,824 + +2.2 5,296,825 - +0.9 5,296,825 - +0.8 5,296,825 - +2.5 5,296,825 - +0.8 5,296,825 - -1.7 5,296,891 + +2.2 5,296,963 + -1.7 5,296,964 - -1.2 5,297,033 + -2.4 5,297,033 + -2.0 5,297,035 + -1.2 5,297,036 - -1.5 5,297,036 - +2.2 5,297,063 - AO356_23605 0.11 +2.3 5,297,165 - AO356_23605 0.17 +1.5 5,297,165 - AO356_23605 0.17 -0.1 5,297,437 + AO356_23605 0.32 -2.2 5,297,450 - AO356_23605 0.33 -2.4 5,297,563 - AO356_23605 0.39 +0.3 5,297,602 + AO356_23605 0.41 +0.1 5,297,602 + AO356_23605 0.41 +0.6 5,297,608 + AO356_23605 0.41 -1.9 5,297,608 + AO356_23605 0.41 +0.7 5,297,608 + AO356_23605 0.41 -0.9 5,297,608 + AO356_23605 0.41 +2.5 5,297,609 - AO356_23605 0.41 +0.6 5,297,609 - AO356_23605 0.41 +1.0 5,297,609 - AO356_23605 0.41 -0.7 5,297,609 - AO356_23605 0.41 +0.6 5,297,609 - AO356_23605 0.41 +1.0 5,297,609 - AO356_23605 0.41 +0.6 5,297,609 - AO356_23605 0.41 +1.3 5,297,609 - AO356_23605 0.41 -0.1 5,297,609 - AO356_23605 0.41 -1.3 5,297,611 + AO356_23605 0.41 -2.8 5,297,611 + AO356_23605 0.41 +0.4 5,297,686 + AO356_23605 0.46 +0.7 5,297,687 - AO356_23605 0.46 -1.9 5,297,687 - AO356_23605 0.46 +0.9 5,297,803 + AO356_23605 0.52 -1.0
Or see this region's nucleotide sequence