Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_21025 and AO356_21030 are separated by 203 nucleotides AO356_21030 and AO356_21035 are separated by 26 nucleotides AO356_21035 and AO356_21040 are separated by 72 nucleotides
AO356_21025: AO356_21025 - AraC family transcriptional regulator, at 4,678,147 to 4,679,094
_21025
AO356_21030: AO356_21030 - oxidoreductase, at 4,679,298 to 4,680,035
_21030
AO356_21035: AO356_21035 - hypothetical protein, at 4,680,062 to 4,680,319
_21035
AO356_21040: AO356_21040 - dimethylglycine dehydrogenase, at 4,680,392 to 4,681,078
_21040
Position (kb)
4679
4680
4681 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4678.448 kb on + strand, within AO356_21025 at 4678.770 kb on - strand, within AO356_21025 at 4678.835 kb on + strand, within AO356_21025 at 4678.835 kb on + strand, within AO356_21025 at 4678.835 kb on + strand, within AO356_21025 at 4678.835 kb on + strand, within AO356_21025 at 4678.836 kb on - strand, within AO356_21025 at 4678.836 kb on - strand, within AO356_21025 at 4678.850 kb on + strand, within AO356_21025 at 4678.851 kb on - strand, within AO356_21025 at 4678.851 kb on - strand, within AO356_21025 at 4678.851 kb on - strand, within AO356_21025 at 4678.851 kb on - strand, within AO356_21025 at 4678.851 kb on - strand, within AO356_21025 at 4678.851 kb on - strand, within AO356_21025 at 4678.856 kb on + strand, within AO356_21025 at 4678.856 kb on + strand, within AO356_21025 at 4678.856 kb on + strand, within AO356_21025 at 4678.856 kb on + strand, within AO356_21025 at 4678.857 kb on - strand, within AO356_21025 at 4678.857 kb on - strand, within AO356_21025 at 4678.857 kb on - strand, within AO356_21025 at 4678.857 kb on - strand, within AO356_21025 at 4678.857 kb on - strand, within AO356_21025 at 4678.857 kb on - strand, within AO356_21025 at 4678.857 kb on - strand, within AO356_21025 at 4679.086 kb on + strand at 4679.124 kb on - strand at 4679.136 kb on + strand at 4679.137 kb on - strand at 4679.173 kb on + strand at 4679.173 kb on + strand at 4679.174 kb on - strand at 4679.180 kb on - strand at 4679.237 kb on + strand at 4679.275 kb on + strand at 4679.276 kb on - strand at 4679.276 kb on - strand at 4679.660 kb on + strand, within AO356_21030 at 4679.760 kb on + strand, within AO356_21030 at 4679.775 kb on + strand, within AO356_21030 at 4679.775 kb on + strand, within AO356_21030 at 4679.776 kb on - strand, within AO356_21030 at 4679.989 kb on + strand at 4679.989 kb on + strand at 4680.088 kb on + strand, within AO356_21035 at 4680.155 kb on + strand, within AO356_21035 at 4680.155 kb on + strand, within AO356_21035 at 4680.155 kb on + strand, within AO356_21035 at 4680.156 kb on - strand, within AO356_21035 at 4680.174 kb on + strand, within AO356_21035 at 4680.175 kb on - strand, within AO356_21035 at 4680.175 kb on - strand, within AO356_21035 at 4680.188 kb on + strand, within AO356_21035 at 4680.189 kb on - strand, within AO356_21035 at 4680.201 kb on - strand, within AO356_21035 at 4680.207 kb on + strand, within AO356_21035 at 4680.228 kb on - strand, within AO356_21035 at 4680.228 kb on - strand, within AO356_21035 at 4680.281 kb on + strand, within AO356_21035 at 4680.302 kb on + strand at 4680.303 kb on - strand at 4680.303 kb on - strand at 4680.303 kb on - strand at 4680.303 kb on - strand at 4680.538 kb on - strand, within AO356_21040 at 4680.660 kb on - strand, within AO356_21040 at 4680.709 kb on - strand, within AO356_21040 at 4680.787 kb on + strand, within AO356_21040 at 4680.787 kb on + strand, within AO356_21040 at 4680.788 kb on - strand, within AO356_21040 at 4680.788 kb on - strand, within AO356_21040 at 4680.788 kb on - strand, within AO356_21040 at 4680.792 kb on + strand, within AO356_21040 at 4680.792 kb on + strand, within AO356_21040 at 4680.793 kb on - strand, within AO356_21040 at 4681.014 kb on + strand at 4681.014 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,678,448 + AO356_21025 0.32 -0.4 4,678,770 - AO356_21025 0.66 -0.9 4,678,835 + AO356_21025 0.73 +0.4 4,678,835 + AO356_21025 0.73 +0.4 4,678,835 + AO356_21025 0.73 -0.1 4,678,835 + AO356_21025 0.73 +0.4 4,678,836 - AO356_21025 0.73 -0.3 4,678,836 - AO356_21025 0.73 -0.2 4,678,850 + AO356_21025 0.74 +3.5 4,678,851 - AO356_21025 0.74 -1.2 4,678,851 - AO356_21025 0.74 +0.5 4,678,851 - AO356_21025 0.74 -0.6 4,678,851 - AO356_21025 0.74 -1.1 4,678,851 - AO356_21025 0.74 +2.1 4,678,851 - AO356_21025 0.74 +2.5 4,678,856 + AO356_21025 0.75 -0.6 4,678,856 + AO356_21025 0.75 -2.9 4,678,856 + AO356_21025 0.75 +0.3 4,678,856 + AO356_21025 0.75 +2.1 4,678,857 - AO356_21025 0.75 +1.1 4,678,857 - AO356_21025 0.75 -0.1 4,678,857 - AO356_21025 0.75 +0.4 4,678,857 - AO356_21025 0.75 -0.7 4,678,857 - AO356_21025 0.75 -2.3 4,678,857 - AO356_21025 0.75 -3.1 4,678,857 - AO356_21025 0.75 +2.8 4,679,086 + +1.9 4,679,124 - +0.5 4,679,136 + -1.9 4,679,137 - -0.4 4,679,173 + -0.5 4,679,173 + +1.1 4,679,174 - +1.9 4,679,180 - -0.4 4,679,237 + +0.1 4,679,275 + +0.6 4,679,276 - -2.1 4,679,276 - -0.1 4,679,660 + AO356_21030 0.49 +2.1 4,679,760 + AO356_21030 0.63 +1.2 4,679,775 + AO356_21030 0.65 -0.1 4,679,775 + AO356_21030 0.65 -0.7 4,679,776 - AO356_21030 0.65 +0.8 4,679,989 + -0.1 4,679,989 + +1.2 4,680,088 + AO356_21035 0.10 -0.6 4,680,155 + AO356_21035 0.36 -1.8 4,680,155 + AO356_21035 0.36 -1.2 4,680,155 + AO356_21035 0.36 +0.3 4,680,156 - AO356_21035 0.36 +1.5 4,680,174 + AO356_21035 0.43 -2.5 4,680,175 - AO356_21035 0.44 +0.5 4,680,175 - AO356_21035 0.44 +0.1 4,680,188 + AO356_21035 0.49 -1.5 4,680,189 - AO356_21035 0.49 +1.0 4,680,201 - AO356_21035 0.54 +0.1 4,680,207 + AO356_21035 0.56 +0.7 4,680,228 - AO356_21035 0.64 -0.9 4,680,228 - AO356_21035 0.64 -0.1 4,680,281 + AO356_21035 0.85 +0.4 4,680,302 + +1.2 4,680,303 - -1.8 4,680,303 - +0.1 4,680,303 - -0.9 4,680,303 - -2.1 4,680,538 - AO356_21040 0.21 -0.4 4,680,660 - AO356_21040 0.39 -0.2 4,680,709 - AO356_21040 0.46 +1.0 4,680,787 + AO356_21040 0.57 +0.7 4,680,787 + AO356_21040 0.57 -0.0 4,680,788 - AO356_21040 0.58 -0.7 4,680,788 - AO356_21040 0.58 +1.3 4,680,788 - AO356_21040 0.58 +1.0 4,680,792 + AO356_21040 0.58 +1.1 4,680,792 + AO356_21040 0.58 +1.1 4,680,793 - AO356_21040 0.58 +0.9 4,681,014 + +0.9 4,681,014 + +0.9
Or see this region's nucleotide sequence