Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_19770 and AO356_19775 are separated by 11 nucleotides AO356_19775 and AO356_19780 are separated by 248 nucleotides AO356_19780 and AO356_19785 overlap by 4 nucleotides AO356_19785 and AO356_19790 are separated by 0 nucleotides
AO356_19770: AO356_19770 - 3-oxoacyl-ACP synthase, at 4,433,364 to 4,434,584
_19770
AO356_19775: AO356_19775 - 3-hydroxydecanoyl-ACP dehydratase, at 4,434,596 to 4,435,111
_19775
AO356_19780: AO356_19780 - histidine kinase, at 4,435,360 to 4,437,261
_19780
AO356_19785: AO356_19785 - ABC transporter substrate-binding protein, at 4,437,258 to 4,438,190
_19785
AO356_19790: AO356_19790 - TonB-dependent receptor, at 4,438,191 to 4,440,314
_19790
Position (kb)
4435
4436
4437
4438 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 4435.126 kb on - strand at 4435.196 kb on - strand at 4435.249 kb on - strand at 4435.361 kb on + strand at 4435.361 kb on + strand at 4435.361 kb on + strand at 4435.362 kb on - strand at 4435.362 kb on - strand at 4435.362 kb on - strand at 4435.362 kb on - strand at 4435.362 kb on - strand at 4435.422 kb on - strand at 4435.422 kb on - strand at 4435.609 kb on + strand, within AO356_19780 at 4435.610 kb on - strand, within AO356_19780 at 4435.907 kb on + strand, within AO356_19780 at 4435.977 kb on + strand, within AO356_19780 at 4435.977 kb on + strand, within AO356_19780 at 4435.978 kb on - strand, within AO356_19780 at 4435.978 kb on - strand, within AO356_19780 at 4436.357 kb on + strand, within AO356_19780 at 4436.413 kb on + strand, within AO356_19780 at 4436.413 kb on + strand, within AO356_19780 at 4436.630 kb on + strand, within AO356_19780 at 4436.631 kb on - strand, within AO356_19780 at 4436.631 kb on - strand, within AO356_19780 at 4436.826 kb on - strand, within AO356_19780 at 4436.826 kb on - strand, within AO356_19780 at 4436.966 kb on + strand, within AO356_19780 at 4436.966 kb on + strand, within AO356_19780 at 4436.966 kb on + strand, within AO356_19780 at 4436.966 kb on + strand, within AO356_19780 at 4436.967 kb on - strand, within AO356_19780 at 4436.967 kb on - strand, within AO356_19780 at 4436.967 kb on - strand, within AO356_19780 at 4436.967 kb on - strand, within AO356_19780 at 4436.967 kb on - strand, within AO356_19780 at 4436.967 kb on - strand, within AO356_19780 at 4436.967 kb on - strand, within AO356_19780 at 4437.010 kb on + strand, within AO356_19780 at 4437.010 kb on + strand, within AO356_19780 at 4437.010 kb on + strand, within AO356_19780 at 4437.011 kb on - strand, within AO356_19780 at 4437.011 kb on - strand, within AO356_19780 at 4437.080 kb on + strand at 4437.081 kb on - strand at 4437.166 kb on - strand at 4437.231 kb on + strand at 4437.451 kb on + strand, within AO356_19785 at 4437.451 kb on + strand, within AO356_19785 at 4437.451 kb on + strand, within AO356_19785 at 4437.452 kb on - strand, within AO356_19785 at 4437.452 kb on - strand, within AO356_19785 at 4437.520 kb on + strand, within AO356_19785 at 4437.520 kb on + strand, within AO356_19785 at 4437.520 kb on + strand, within AO356_19785 at 4437.521 kb on - strand, within AO356_19785 at 4437.521 kb on - strand, within AO356_19785 at 4437.521 kb on - strand, within AO356_19785 at 4437.559 kb on + strand, within AO356_19785 at 4437.559 kb on + strand, within AO356_19785 at 4437.560 kb on - strand, within AO356_19785 at 4437.560 kb on - strand, within AO356_19785 at 4437.588 kb on + strand, within AO356_19785 at 4437.588 kb on + strand, within AO356_19785 at 4437.588 kb on + strand, within AO356_19785 at 4437.588 kb on + strand, within AO356_19785 at 4437.724 kb on + strand, within AO356_19785 at 4437.992 kb on + strand, within AO356_19785 at 4437.993 kb on - strand, within AO356_19785 at 4437.993 kb on - strand, within AO356_19785 at 4438.192 kb on + strand at 4438.192 kb on + strand at 4438.192 kb on + strand at 4438.192 kb on + strand at 4438.192 kb on + strand at 4438.192 kb on + strand at 4438.216 kb on + strand at 4438.217 kb on - strand at 4438.217 kb on - strand at 4438.217 kb on - strand at 4438.217 kb on - strand at 4438.217 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,435,126 - -1.0 4,435,196 - -0.6 4,435,249 - +0.1 4,435,361 + -0.7 4,435,361 + +0.4 4,435,361 + +1.0 4,435,362 - +1.5 4,435,362 - +2.4 4,435,362 - +2.4 4,435,362 - +1.3 4,435,362 - -1.0 4,435,422 - +2.9 4,435,422 - +2.9 4,435,609 + AO356_19780 0.13 +2.8 4,435,610 - AO356_19780 0.13 +2.8 4,435,907 + AO356_19780 0.29 +2.3 4,435,977 + AO356_19780 0.32 +1.3 4,435,977 + AO356_19780 0.32 +4.3 4,435,978 - AO356_19780 0.32 +2.8 4,435,978 - AO356_19780 0.32 +2.7 4,436,357 + AO356_19780 0.52 +3.9 4,436,413 + AO356_19780 0.55 +2.8 4,436,413 + AO356_19780 0.55 +3.6 4,436,630 + AO356_19780 0.67 +5.2 4,436,631 - AO356_19780 0.67 +1.5 4,436,631 - AO356_19780 0.67 +3.0 4,436,826 - AO356_19780 0.77 +1.7 4,436,826 - AO356_19780 0.77 +4.4 4,436,966 + AO356_19780 0.84 +3.3 4,436,966 + AO356_19780 0.84 +2.4 4,436,966 + AO356_19780 0.84 +2.7 4,436,966 + AO356_19780 0.84 +1.8 4,436,967 - AO356_19780 0.84 +3.0 4,436,967 - AO356_19780 0.84 +2.5 4,436,967 - AO356_19780 0.84 +3.3 4,436,967 - AO356_19780 0.84 +0.1 4,436,967 - AO356_19780 0.84 +1.4 4,436,967 - AO356_19780 0.84 +0.9 4,436,967 - AO356_19780 0.84 +3.5 4,437,010 + AO356_19780 0.87 +3.6 4,437,010 + AO356_19780 0.87 +2.0 4,437,010 + AO356_19780 0.87 +0.3 4,437,011 - AO356_19780 0.87 +1.6 4,437,011 - AO356_19780 0.87 +1.9 4,437,080 + +2.8 4,437,081 - +2.7 4,437,166 - +2.6 4,437,231 + +4.4 4,437,451 + AO356_19785 0.21 -0.2 4,437,451 + AO356_19785 0.21 +1.1 4,437,451 + AO356_19785 0.21 +0.7 4,437,452 - AO356_19785 0.21 -2.8 4,437,452 - AO356_19785 0.21 -0.6 4,437,520 + AO356_19785 0.28 +1.6 4,437,520 + AO356_19785 0.28 +2.4 4,437,520 + AO356_19785 0.28 +2.1 4,437,521 - AO356_19785 0.28 -0.6 4,437,521 - AO356_19785 0.28 -0.4 4,437,521 - AO356_19785 0.28 +1.2 4,437,559 + AO356_19785 0.32 +1.7 4,437,559 + AO356_19785 0.32 +3.4 4,437,560 - AO356_19785 0.32 -2.2 4,437,560 - AO356_19785 0.32 -0.6 4,437,588 + AO356_19785 0.35 -0.1 4,437,588 + AO356_19785 0.35 +2.2 4,437,588 + AO356_19785 0.35 +2.1 4,437,588 + AO356_19785 0.35 +2.3 4,437,724 + AO356_19785 0.50 +0.9 4,437,992 + AO356_19785 0.79 +3.2 4,437,993 - AO356_19785 0.79 -2.2 4,437,993 - AO356_19785 0.79 +1.6 4,438,192 + +3.8 4,438,192 + +1.5 4,438,192 + +2.2 4,438,192 + +2.4 4,438,192 + +1.1 4,438,192 + +1.3 4,438,216 + +1.2 4,438,217 - +0.7 4,438,217 - +2.7 4,438,217 - +1.0 4,438,217 - -0.7 4,438,217 - -0.8
Or see this region's nucleotide sequence