Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_05730 and AO356_05735 overlap by 1 nucleotides AO356_05735 and AO356_05740 are separated by 254 nucleotides AO356_05740 and AO356_05745 are separated by 3 nucleotides
AO356_05730: AO356_05730 - hypothetical protein, at 1,276,425 to 1,276,823
_05730
AO356_05735: AO356_05735 - hypothetical protein, at 1,276,823 to 1,277,332
_05735
AO356_05740: AO356_05740 - baseplate protein, at 1,277,587 to 1,278,627
_05740
AO356_05745: AO356_05745 - hydroxyacid dehydrogenase, at 1,278,631 to 1,279,869
_05745
Position (kb)
1277
1278
1279 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1276.868 kb on + strand at 1276.868 kb on + strand at 1276.869 kb on - strand at 1277.061 kb on + strand, within AO356_05735 at 1277.324 kb on + strand at 1277.325 kb on - strand at 1277.623 kb on - strand at 1277.623 kb on - strand at 1277.675 kb on + strand at 1277.675 kb on + strand at 1277.675 kb on + strand at 1277.675 kb on + strand at 1277.675 kb on + strand at 1277.735 kb on - strand, within AO356_05740 at 1277.792 kb on + strand, within AO356_05740 at 1277.792 kb on + strand, within AO356_05740 at 1277.792 kb on + strand, within AO356_05740 at 1277.792 kb on + strand, within AO356_05740 at 1277.793 kb on - strand, within AO356_05740 at 1277.793 kb on - strand, within AO356_05740 at 1277.996 kb on + strand, within AO356_05740 at 1278.018 kb on - strand, within AO356_05740 at 1278.018 kb on - strand, within AO356_05740 at 1278.018 kb on - strand, within AO356_05740 at 1278.064 kb on + strand, within AO356_05740 at 1278.064 kb on + strand, within AO356_05740 at 1278.064 kb on + strand, within AO356_05740 at 1278.065 kb on - strand, within AO356_05740 at 1278.065 kb on - strand, within AO356_05740 at 1278.065 kb on - strand, within AO356_05740 at 1278.065 kb on - strand, within AO356_05740 at 1278.065 kb on - strand, within AO356_05740 at 1278.065 kb on - strand, within AO356_05740 at 1278.390 kb on - strand, within AO356_05740 at 1278.390 kb on - strand, within AO356_05740 at 1278.390 kb on - strand, within AO356_05740 at 1278.390 kb on - strand, within AO356_05740 at 1278.413 kb on + strand, within AO356_05740 at 1278.414 kb on - strand, within AO356_05740 at 1278.414 kb on - strand, within AO356_05740 at 1278.598 kb on - strand at 1278.598 kb on - strand at 1278.598 kb on - strand at 1278.598 kb on - strand at 1278.633 kb on - strand at 1278.691 kb on - strand at 1278.737 kb on + strand at 1278.738 kb on - strand at 1278.858 kb on - strand, within AO356_05745 at 1279.307 kb on + strand, within AO356_05745 at 1279.349 kb on + strand, within AO356_05745 at 1279.349 kb on + strand, within AO356_05745 at 1279.349 kb on + strand, within AO356_05745 at 1279.350 kb on - strand, within AO356_05745 at 1279.350 kb on - strand, within AO356_05745
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,276,868 + +0.8 1,276,868 + -0.0 1,276,869 - +0.9 1,277,061 + AO356_05735 0.47 +1.4 1,277,324 + +1.5 1,277,325 - -0.9 1,277,623 - +1.3 1,277,623 - -1.2 1,277,675 + -1.8 1,277,675 + +0.8 1,277,675 + +0.9 1,277,675 + +0.2 1,277,675 + +0.2 1,277,735 - AO356_05740 0.14 -0.5 1,277,792 + AO356_05740 0.20 +1.5 1,277,792 + AO356_05740 0.20 -0.6 1,277,792 + AO356_05740 0.20 +0.7 1,277,792 + AO356_05740 0.20 +0.4 1,277,793 - AO356_05740 0.20 +1.8 1,277,793 - AO356_05740 0.20 +0.5 1,277,996 + AO356_05740 0.39 +0.7 1,278,018 - AO356_05740 0.41 +1.0 1,278,018 - AO356_05740 0.41 -0.0 1,278,018 - AO356_05740 0.41 +0.9 1,278,064 + AO356_05740 0.46 -1.9 1,278,064 + AO356_05740 0.46 -0.7 1,278,064 + AO356_05740 0.46 +0.6 1,278,065 - AO356_05740 0.46 +0.5 1,278,065 - AO356_05740 0.46 -3.6 1,278,065 - AO356_05740 0.46 +0.1 1,278,065 - AO356_05740 0.46 -0.8 1,278,065 - AO356_05740 0.46 +0.1 1,278,065 - AO356_05740 0.46 +1.0 1,278,390 - AO356_05740 0.77 -1.8 1,278,390 - AO356_05740 0.77 -0.6 1,278,390 - AO356_05740 0.77 -1.7 1,278,390 - AO356_05740 0.77 +1.1 1,278,413 + AO356_05740 0.79 +0.6 1,278,414 - AO356_05740 0.79 -0.4 1,278,414 - AO356_05740 0.79 -0.1 1,278,598 - -2.3 1,278,598 - -1.6 1,278,598 - -0.4 1,278,598 - +0.3 1,278,633 - -2.3 1,278,691 - +1.5 1,278,737 + -1.5 1,278,738 - +2.7 1,278,858 - AO356_05745 0.18 +0.5 1,279,307 + AO356_05745 0.55 +1.1 1,279,349 + AO356_05745 0.58 -1.5 1,279,349 + AO356_05745 0.58 +0.7 1,279,349 + AO356_05745 0.58 +0.9 1,279,350 - AO356_05745 0.58 -2.5 1,279,350 - AO356_05745 0.58 +0.6
Or see this region's nucleotide sequence