Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_02470 and AO356_02475 overlap by 8 nucleotides AO356_02475 and AO356_02480 are separated by 221 nucleotides AO356_02480 and AO356_02485 are separated by 94 nucleotides AO356_02485 and AO356_02490 are separated by 76 nucleotides
AO356_02470: AO356_02470 - cytochrome C oxidase Cbb3, at 537,552 to 537,770
_02470
AO356_02475: AO356_02475 - cytochrome biogenesis protein, at 537,763 to 538,446
_02475
AO356_02480: AO356_02480 - coproporphyrinogen III oxidase, at 538,668 to 540,050
_02480
AO356_02485: AO356_02485 - transcriptional regulator, at 540,145 to 540,879
_02485
AO356_02490: AO356_02490 - adenine phosphoribosyltransferase, at 540,956 to 541,504
_02490
Position (kb) 538
539
540
541 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 537.940 kb on + strand, within AO356_02475 at 537.940 kb on + strand, within AO356_02475 at 538.547 kb on + strand at 538.547 kb on + strand at 538.547 kb on + strand at 538.547 kb on + strand at 538.547 kb on + strand at 538.547 kb on + strand at 538.547 kb on + strand at 538.547 kb on + strand at 538.547 kb on + strand at 538.548 kb on - strand at 538.548 kb on - strand at 538.548 kb on - strand at 538.629 kb on + strand at 538.629 kb on + strand at 538.651 kb on - strand at 538.710 kb on + strand at 538.731 kb on + strand at 538.731 kb on + strand at 538.732 kb on - strand at 538.732 kb on - strand at 538.732 kb on - strand at 538.836 kb on + strand, within AO356_02480 at 538.837 kb on - strand, within AO356_02480 at 538.869 kb on + strand, within AO356_02480 at 538.869 kb on + strand, within AO356_02480 at 538.869 kb on + strand, within AO356_02480 at 538.870 kb on - strand, within AO356_02480 at 538.870 kb on - strand, within AO356_02480 at 538.872 kb on + strand, within AO356_02480 at 538.873 kb on - strand, within AO356_02480 at 538.873 kb on - strand, within AO356_02480 at 538.873 kb on - strand, within AO356_02480 at 538.923 kb on + strand, within AO356_02480 at 538.923 kb on + strand, within AO356_02480 at 538.924 kb on - strand, within AO356_02480 at 539.313 kb on + strand, within AO356_02480 at 539.314 kb on - strand, within AO356_02480 at 539.404 kb on - strand, within AO356_02480 at 539.404 kb on - strand, within AO356_02480 at 539.529 kb on + strand, within AO356_02480 at 539.530 kb on - strand, within AO356_02480 at 539.530 kb on - strand, within AO356_02480 at 539.530 kb on - strand, within AO356_02480 at 539.616 kb on + strand, within AO356_02480 at 539.616 kb on + strand, within AO356_02480 at 539.617 kb on - strand, within AO356_02480 at 539.617 kb on - strand, within AO356_02480 at 539.617 kb on - strand, within AO356_02480 at 539.617 kb on - strand, within AO356_02480 at 539.685 kb on + strand, within AO356_02480 at 539.685 kb on + strand, within AO356_02480 at 539.716 kb on - strand, within AO356_02480 at 539.874 kb on + strand, within AO356_02480 at 540.006 kb on + strand at 540.006 kb on + strand at 540.006 kb on + strand at 540.007 kb on - strand at 540.007 kb on - strand at 540.007 kb on - strand at 540.044 kb on + strand at 540.093 kb on - strand at 540.115 kb on + strand at 540.116 kb on - strand at 540.116 kb on - strand at 540.116 kb on - strand at 540.116 kb on - strand at 540.366 kb on + strand, within AO356_02485 at 540.367 kb on - strand, within AO356_02485 at 540.530 kb on + strand, within AO356_02485 at 540.531 kb on - strand, within AO356_02485 at 540.606 kb on - strand, within AO356_02485 at 540.736 kb on + strand, within AO356_02485 at 540.793 kb on + strand, within AO356_02485 at 540.918 kb on + strand at 540.918 kb on + strand at 540.918 kb on + strand at 540.918 kb on + strand at 540.918 kb on + strand at 540.919 kb on - strand at 540.919 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 537,940 + AO356_02475 0.26 -1.7 537,940 + AO356_02475 0.26 -0.7 538,547 + -1.9 538,547 + -0.5 538,547 + -2.4 538,547 + +0.5 538,547 + -1.0 538,547 + +2.5 538,547 + +0.8 538,547 + +1.0 538,547 + +0.7 538,548 - +0.2 538,548 - +1.0 538,548 - -0.5 538,629 + +1.3 538,629 + +1.4 538,651 - +0.0 538,710 + -0.8 538,731 + -0.5 538,731 + -0.2 538,732 - -2.4 538,732 - -0.6 538,732 - +0.8 538,836 + AO356_02480 0.12 +0.4 538,837 - AO356_02480 0.12 +1.3 538,869 + AO356_02480 0.15 -0.3 538,869 + AO356_02480 0.15 +0.0 538,869 + AO356_02480 0.15 -1.1 538,870 - AO356_02480 0.15 -0.3 538,870 - AO356_02480 0.15 +0.9 538,872 + AO356_02480 0.15 -0.1 538,873 - AO356_02480 0.15 -0.4 538,873 - AO356_02480 0.15 -1.6 538,873 - AO356_02480 0.15 -0.5 538,923 + AO356_02480 0.18 +2.0 538,923 + AO356_02480 0.18 +1.9 538,924 - AO356_02480 0.19 -1.3 539,313 + AO356_02480 0.47 -0.8 539,314 - AO356_02480 0.47 -1.9 539,404 - AO356_02480 0.53 -0.8 539,404 - AO356_02480 0.53 -1.9 539,529 + AO356_02480 0.62 -0.7 539,530 - AO356_02480 0.62 -2.0 539,530 - AO356_02480 0.62 -3.1 539,530 - AO356_02480 0.62 -0.4 539,616 + AO356_02480 0.69 +0.3 539,616 + AO356_02480 0.69 -0.6 539,617 - AO356_02480 0.69 +0.4 539,617 - AO356_02480 0.69 +0.5 539,617 - AO356_02480 0.69 -3.3 539,617 - AO356_02480 0.69 -1.1 539,685 + AO356_02480 0.74 -1.0 539,685 + AO356_02480 0.74 +1.0 539,716 - AO356_02480 0.76 -2.4 539,874 + AO356_02480 0.87 -2.0 540,006 + +0.6 540,006 + -1.4 540,006 + -1.8 540,007 - +0.1 540,007 - +0.3 540,007 - +1.9 540,044 + +0.9 540,093 - -2.0 540,115 + +0.6 540,116 - -2.2 540,116 - +3.2 540,116 - -2.2 540,116 - -1.9 540,366 + AO356_02485 0.30 -1.0 540,367 - AO356_02485 0.30 -0.5 540,530 + AO356_02485 0.52 +0.2 540,531 - AO356_02485 0.53 -0.6 540,606 - AO356_02485 0.63 -1.1 540,736 + AO356_02485 0.80 -0.2 540,793 + AO356_02485 0.88 +1.1 540,918 + +1.2 540,918 + +0.1 540,918 + +1.8 540,918 + +0.4 540,918 + +1.0 540,919 - -0.0 540,919 - +0.4
Or see this region's nucleotide sequence