Strain Fitness in Pseudomonas fluorescens FW300-N2C3 around AO356_00285

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO356_00275 and AO356_00280 overlap by 12 nucleotidesAO356_00280 and AO356_00285 are separated by 460 nucleotidesAO356_00285 and AO356_00290 are separated by 130 nucleotides AO356_00275: AO356_00275 - hypothetical protein, at 50,075 to 51,616 _00275 AO356_00280: AO356_00280 - hypothetical protein, at 51,605 to 52,000 _00280 AO356_00285: AO356_00285 - hypothetical protein, at 52,461 to 52,790 _00285 AO356_00290: AO356_00290 - DNA primase, at 52,921 to 53,883 _00290 Position (kb) 52 53Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 51.467 kb on - strandat 51.467 kb on - strandat 51.501 kb on - strandat 51.574 kb on + strandat 51.574 kb on + strandat 51.575 kb on - strandat 51.611 kb on - strandat 51.611 kb on - strandat 51.633 kb on + strandat 51.634 kb on - strandat 51.664 kb on - strand, within AO356_00280at 51.664 kb on - strand, within AO356_00280at 51.664 kb on - strand, within AO356_00280at 51.682 kb on - strand, within AO356_00280at 51.706 kb on + strand, within AO356_00280at 51.706 kb on + strand, within AO356_00280at 51.706 kb on + strand, within AO356_00280at 51.707 kb on - strand, within AO356_00280at 51.707 kb on - strand, within AO356_00280at 51.707 kb on - strand, within AO356_00280at 51.707 kb on - strand, within AO356_00280at 51.707 kb on - strand, within AO356_00280at 51.940 kb on + strand, within AO356_00280at 51.941 kb on - strand, within AO356_00280at 51.951 kb on + strand, within AO356_00280at 51.951 kb on + strand, within AO356_00280at 52.022 kb on + strandat 52.050 kb on + strandat 52.050 kb on + strandat 52.050 kb on + strandat 52.050 kb on + strandat 52.050 kb on + strandat 52.051 kb on - strandat 52.051 kb on - strandat 52.051 kb on - strandat 52.051 kb on - strandat 52.051 kb on - strandat 52.053 kb on + strandat 52.149 kb on + strandat 52.170 kb on - strandat 52.170 kb on - strandat 52.180 kb on + strandat 52.180 kb on + strandat 52.180 kb on + strandat 52.181 kb on - strandat 52.181 kb on - strandat 52.184 kb on + strandat 52.191 kb on + strandat 52.245 kb on - strandat 52.245 kb on - strandat 52.312 kb on - strandat 52.319 kb on + strandat 52.455 kb on + strandat 52.455 kb on + strandat 52.455 kb on + strandat 52.455 kb on + strandat 52.456 kb on - strandat 52.456 kb on - strandat 52.471 kb on + strandat 52.543 kb on + strand, within AO356_00285at 52.544 kb on - strand, within AO356_00285at 52.647 kb on + strand, within AO356_00285at 52.647 kb on + strand, within AO356_00285at 52.647 kb on + strand, within AO356_00285at 52.648 kb on - strand, within AO356_00285at 52.648 kb on - strand, within AO356_00285at 52.683 kb on - strand, within AO356_00285at 52.749 kb on - strand, within AO356_00285at 52.831 kb on - strandat 52.831 kb on - strandat 52.832 kb on + strandat 52.832 kb on + strandat 52.930 kb on - strandat 52.930 kb on - strandat 52.933 kb on + strandat 52.933 kb on + strandat 52.933 kb on + strandat 52.934 kb on - strandat 52.934 kb on - strandat 52.968 kb on - strandat 53.213 kb on - strand, within AO356_00290at 53.213 kb on - strand, within AO356_00290at 53.426 kb on + strand, within AO356_00290at 53.426 kb on + strand, within AO356_00290at 53.426 kb on + strand, within AO356_00290at 53.427 kb on - strand, within AO356_00290at 53.442 kb on + strand, within AO356_00290at 53.442 kb on + strand, within AO356_00290at 53.537 kb on - strand, within AO356_00290at 53.539 kb on + strand, within AO356_00290at 53.539 kb on + strand, within AO356_00290at 53.540 kb on - strand, within AO356_00290at 53.638 kb on + strand, within AO356_00290at 53.682 kb on - strand, within AO356_00290at 53.689 kb on + strand, within AO356_00290at 53.690 kb on - strand, within AO356_00290

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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51,467 - -0.9
51,467 - +0.8
51,501 - +0.1
51,574 + +0.1
51,574 + +0.8
51,575 - +2.4
51,611 - +0.2
51,611 - -1.3
51,633 + -0.5
51,634 - -1.9
51,664 - AO356_00280 0.15 -0.5
51,664 - AO356_00280 0.15 -0.1
51,664 - AO356_00280 0.15 -1.3
51,682 - AO356_00280 0.19 -2.3
51,706 + AO356_00280 0.26 +0.8
51,706 + AO356_00280 0.26 -0.1
51,706 + AO356_00280 0.26 -0.3
51,707 - AO356_00280 0.26 +1.9
51,707 - AO356_00280 0.26 +1.4
51,707 - AO356_00280 0.26 -1.1
51,707 - AO356_00280 0.26 -0.8
51,707 - AO356_00280 0.26 -1.5
51,940 + AO356_00280 0.85 -1.1
51,941 - AO356_00280 0.85 +1.4
51,951 + AO356_00280 0.87 +1.6
51,951 + AO356_00280 0.87 -0.4
52,022 + -0.3
52,050 + +0.2
52,050 + +0.9
52,050 + -0.8
52,050 + +0.5
52,050 + +0.7
52,051 - -0.3
52,051 - +0.5
52,051 - -0.7
52,051 - +0.3
52,051 - +0.7
52,053 + -0.2
52,149 + -0.0
52,170 - +0.7
52,170 - +1.1
52,180 + +0.9
52,180 + +0.5
52,180 + -1.6
52,181 - -0.3
52,181 - -1.1
52,184 + +2.2
52,191 + +0.1
52,245 - -0.9
52,245 - +1.0
52,312 - -2.6
52,319 + -2.7
52,455 + +2.0
52,455 + -1.7
52,455 + +1.9
52,455 + +0.1
52,456 - +1.9
52,456 - -2.6
52,471 + -0.1
52,543 + AO356_00285 0.25 -1.0
52,544 - AO356_00285 0.25 -1.6
52,647 + AO356_00285 0.56 -1.2
52,647 + AO356_00285 0.56 -1.8
52,647 + AO356_00285 0.56 -0.0
52,648 - AO356_00285 0.57 -3.5
52,648 - AO356_00285 0.57 -0.3
52,683 - AO356_00285 0.67 -0.0
52,749 - AO356_00285 0.87 +0.2
52,831 - -0.6
52,831 - +0.3
52,832 + -0.5
52,832 + -1.1
52,930 - -0.8
52,930 - +0.8
52,933 + +0.5
52,933 + -1.3
52,933 + +1.7
52,934 - +0.4
52,934 - -0.4
52,968 - +0.6
53,213 - AO356_00290 0.30 -1.4
53,213 - AO356_00290 0.30 -0.1
53,426 + AO356_00290 0.52 +2.1
53,426 + AO356_00290 0.52 -0.3
53,426 + AO356_00290 0.52 -0.8
53,427 - AO356_00290 0.53 +2.0
53,442 + AO356_00290 0.54 -1.9
53,442 + AO356_00290 0.54 -1.3
53,537 - AO356_00290 0.64 -0.8
53,539 + AO356_00290 0.64 -0.4
53,539 + AO356_00290 0.64 -0.4
53,540 - AO356_00290 0.64 +1.5
53,638 + AO356_00290 0.74 -1.0
53,682 - AO356_00290 0.79 +0.6
53,689 + AO356_00290 0.80 -0.5
53,690 - AO356_00290 0.80 +0.7

Or see this region's nucleotide sequence