Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_02520 and AO353_02525 are separated by 118 nucleotides AO353_02525 and AO353_02530 are separated by 91 nucleotides AO353_02530 and AO353_02535 are separated by 84 nucleotides
AO353_02520: AO353_02520 - sodium transporter, at 536,655 to 537,620
_02520
AO353_02525: AO353_02525 - molecular chaperone, at 537,739 to 538,059
_02525
AO353_02530: AO353_02530 - glycerol acyltransferase, at 538,151 to 540,025
_02530
AO353_02535: AO353_02535 - C4-dicarboxylate ABC transporter, at 540,110 to 541,261
_02535
Position (kb) 538
539
540
541 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 537.351 kb on - strand, within AO353_02520 at 537.351 kb on - strand, within AO353_02520 at 537.351 kb on - strand, within AO353_02520 at 537.375 kb on + strand, within AO353_02520 at 537.375 kb on + strand, within AO353_02520 at 537.558 kb on + strand at 537.558 kb on + strand at 537.558 kb on + strand at 537.559 kb on - strand at 537.559 kb on - strand at 537.559 kb on - strand at 537.559 kb on - strand at 537.559 kb on - strand at 537.559 kb on - strand at 537.559 kb on - strand at 537.626 kb on + strand at 537.646 kb on + strand at 537.647 kb on - strand at 537.656 kb on + strand at 537.656 kb on + strand at 537.656 kb on + strand at 537.656 kb on + strand at 537.658 kb on + strand at 537.658 kb on + strand at 537.658 kb on + strand at 537.659 kb on - strand at 537.659 kb on - strand at 537.659 kb on - strand at 537.659 kb on - strand at 537.698 kb on + strand at 537.698 kb on + strand at 537.698 kb on + strand at 537.699 kb on - strand at 537.699 kb on - strand at 537.701 kb on + strand at 537.701 kb on + strand at 537.701 kb on + strand at 537.701 kb on + strand at 537.701 kb on + strand at 537.701 kb on + strand at 537.702 kb on - strand at 537.702 kb on - strand at 537.702 kb on - strand at 537.702 kb on - strand at 537.702 kb on - strand at 537.702 kb on - strand at 537.802 kb on + strand, within AO353_02525 at 537.802 kb on + strand, within AO353_02525 at 537.803 kb on - strand, within AO353_02525 at 537.803 kb on - strand, within AO353_02525 at 537.803 kb on - strand, within AO353_02525 at 537.803 kb on - strand, within AO353_02525 at 537.803 kb on - strand, within AO353_02525 at 537.804 kb on + strand, within AO353_02525 at 537.804 kb on + strand, within AO353_02525 at 537.805 kb on - strand, within AO353_02525 at 537.805 kb on - strand, within AO353_02525 at 537.858 kb on - strand, within AO353_02525 at 537.858 kb on - strand, within AO353_02525 at 537.914 kb on - strand, within AO353_02525 at 537.914 kb on - strand, within AO353_02525 at 538.057 kb on + strand at 538.057 kb on + strand at 538.057 kb on + strand at 538.058 kb on - strand at 538.058 kb on - strand at 538.088 kb on - strand at 538.093 kb on + strand at 538.093 kb on + strand at 538.093 kb on + strand at 538.093 kb on + strand at 538.093 kb on + strand at 538.094 kb on - strand at 538.094 kb on - strand at 538.100 kb on + strand at 538.101 kb on - strand at 538.101 kb on - strand at 538.109 kb on - strand at 538.152 kb on + strand at 538.152 kb on + strand at 538.152 kb on + strand at 538.152 kb on + strand at 538.153 kb on - strand at 538.153 kb on - strand at 538.153 kb on - strand at 538.153 kb on - strand at 538.156 kb on + strand at 538.156 kb on + strand at 538.156 kb on + strand at 538.157 kb on - strand at 538.449 kb on + strand, within AO353_02530 at 538.449 kb on + strand, within AO353_02530 at 538.450 kb on - strand, within AO353_02530 at 538.558 kb on + strand, within AO353_02530 at 538.559 kb on - strand, within AO353_02530 at 538.559 kb on - strand at 538.559 kb on - strand, within AO353_02530 at 538.559 kb on - strand, within AO353_02530 at 538.559 kb on - strand, within AO353_02530 at 538.559 kb on - strand, within AO353_02530 at 538.559 kb on - strand, within AO353_02530 at 538.560 kb on + strand, within AO353_02530 at 538.560 kb on + strand, within AO353_02530 at 538.560 kb on + strand, within AO353_02530 at 538.560 kb on + strand, within AO353_02530 at 538.560 kb on + strand, within AO353_02530 at 538.560 kb on + strand, within AO353_02530 at 538.560 kb on + strand, within AO353_02530 at 538.560 kb on + strand, within AO353_02530 at 538.561 kb on - strand, within AO353_02530 at 538.561 kb on - strand, within AO353_02530 at 538.561 kb on - strand, within AO353_02530 at 538.561 kb on - strand, within AO353_02530 at 538.561 kb on - strand, within AO353_02530 at 538.561 kb on - strand, within AO353_02530 at 538.561 kb on - strand, within AO353_02530 at 538.561 kb on - strand, within AO353_02530 at 538.561 kb on - strand, within AO353_02530 at 538.561 kb on - strand, within AO353_02530 at 538.569 kb on + strand, within AO353_02530 at 538.569 kb on + strand, within AO353_02530 at 538.569 kb on + strand, within AO353_02530 at 538.569 kb on + strand, within AO353_02530 at 538.569 kb on + strand, within AO353_02530 at 538.569 kb on + strand, within AO353_02530 at 538.569 kb on + strand, within AO353_02530 at 538.569 kb on + strand, within AO353_02530 at 538.569 kb on + strand, within AO353_02530 at 538.569 kb on + strand, within AO353_02530 at 538.570 kb on - strand, within AO353_02530 at 538.570 kb on - strand, within AO353_02530 at 538.570 kb on - strand, within AO353_02530 at 538.570 kb on - strand, within AO353_02530 at 538.570 kb on - strand, within AO353_02530 at 538.572 kb on + strand, within AO353_02530 at 538.572 kb on + strand, within AO353_02530 at 538.572 kb on + strand, within AO353_02530 at 538.572 kb on + strand, within AO353_02530 at 538.572 kb on + strand, within AO353_02530 at 538.572 kb on + strand, within AO353_02530 at 538.572 kb on + strand, within AO353_02530 at 538.572 kb on + strand, within AO353_02530 at 538.572 kb on + strand, within AO353_02530 at 538.572 kb on + strand, within AO353_02530 at 538.573 kb on - strand, within AO353_02530 at 538.573 kb on - strand, within AO353_02530 at 538.573 kb on - strand, within AO353_02530 at 538.704 kb on + strand, within AO353_02530 at 538.704 kb on + strand at 538.704 kb on + strand, within AO353_02530 at 538.705 kb on - strand, within AO353_02530 at 538.770 kb on + strand, within AO353_02530 at 538.771 kb on - strand, within AO353_02530 at 538.771 kb on - strand, within AO353_02530 at 538.771 kb on - strand, within AO353_02530 at 538.771 kb on - strand, within AO353_02530 at 538.771 kb on - strand, within AO353_02530 at 538.771 kb on - strand, within AO353_02530 at 538.771 kb on - strand, within AO353_02530 at 538.771 kb on - strand, within AO353_02530 at 538.778 kb on + strand, within AO353_02530 at 538.778 kb on + strand, within AO353_02530 at 538.778 kb on + strand, within AO353_02530 at 538.778 kb on + strand, within AO353_02530 at 538.779 kb on - strand, within AO353_02530 at 538.779 kb on - strand, within AO353_02530 at 538.779 kb on - strand, within AO353_02530 at 538.779 kb on - strand at 538.779 kb on - strand, within AO353_02530 at 538.779 kb on - strand, within AO353_02530 at 538.956 kb on + strand, within AO353_02530 at 538.957 kb on - strand, within AO353_02530 at 538.957 kb on - strand, within AO353_02530 at 538.957 kb on - strand, within AO353_02530 at 538.971 kb on + strand, within AO353_02530 at 538.971 kb on + strand, within AO353_02530 at 539.289 kb on + strand, within AO353_02530 at 539.290 kb on - strand, within AO353_02530 at 539.290 kb on - strand, within AO353_02530 at 539.290 kb on - strand, within AO353_02530 at 539.396 kb on - strand, within AO353_02530 at 539.396 kb on - strand, within AO353_02530 at 539.396 kb on - strand, within AO353_02530 at 539.411 kb on + strand, within AO353_02530 at 539.411 kb on + strand, within AO353_02530 at 539.411 kb on + strand, within AO353_02530 at 539.411 kb on + strand, within AO353_02530 at 539.412 kb on - strand, within AO353_02530 at 539.412 kb on - strand, within AO353_02530 at 539.412 kb on - strand, within AO353_02530 at 539.461 kb on - strand, within AO353_02530 at 539.509 kb on - strand, within AO353_02530 at 539.637 kb on + strand, within AO353_02530 at 539.637 kb on + strand, within AO353_02530 at 539.638 kb on - strand, within AO353_02530 at 539.638 kb on - strand, within AO353_02530 at 539.638 kb on - strand, within AO353_02530 at 539.901 kb on + strand at 539.902 kb on - strand at 539.966 kb on - strand at 540.057 kb on + strand at 540.057 kb on + strand at 540.057 kb on + strand at 540.058 kb on - strand at 540.058 kb on - strand at 540.058 kb on - strand at 540.058 kb on - strand at 540.070 kb on + strand at 540.093 kb on + strand at 540.093 kb on + strand at 540.093 kb on + strand at 540.094 kb on - strand at 540.094 kb on - strand at 540.094 kb on - strand at 540.094 kb on - strand at 540.097 kb on + strand at 540.097 kb on + strand at 540.098 kb on - strand at 540.098 kb on - strand at 540.111 kb on + strand at 540.111 kb on + strand at 540.112 kb on - strand at 540.112 kb on - strand at 540.112 kb on - strand at 540.120 kb on - strand at 540.288 kb on + strand, within AO353_02535 at 540.307 kb on + strand, within AO353_02535 at 540.307 kb on + strand, within AO353_02535 at 540.307 kb on + strand, within AO353_02535 at 540.307 kb on + strand, within AO353_02535 at 540.307 kb on + strand, within AO353_02535 at 540.308 kb on - strand, within AO353_02535 at 540.308 kb on - strand, within AO353_02535 at 540.308 kb on - strand, within AO353_02535 at 540.308 kb on - strand, within AO353_02535 at 540.308 kb on - strand, within AO353_02535 at 540.308 kb on - strand, within AO353_02535 at 540.409 kb on + strand, within AO353_02535 at 540.409 kb on + strand, within AO353_02535 at 540.409 kb on + strand, within AO353_02535 at 540.409 kb on + strand, within AO353_02535 at 540.409 kb on + strand, within AO353_02535 at 540.409 kb on + strand, within AO353_02535 at 540.409 kb on + strand, within AO353_02535 at 540.409 kb on + strand, within AO353_02535 at 540.410 kb on - strand, within AO353_02535 at 540.410 kb on - strand, within AO353_02535 at 540.410 kb on - strand, within AO353_02535 at 540.410 kb on - strand, within AO353_02535 at 540.410 kb on - strand, within AO353_02535 at 540.592 kb on + strand, within AO353_02535 at 540.599 kb on + strand, within AO353_02535 at 540.599 kb on + strand at 540.599 kb on + strand, within AO353_02535 at 540.600 kb on - strand, within AO353_02535 at 540.654 kb on + strand, within AO353_02535 at 540.654 kb on + strand, within AO353_02535 at 540.654 kb on + strand, within AO353_02535 at 540.654 kb on + strand, within AO353_02535 at 540.654 kb on + strand, within AO353_02535 at 540.654 kb on + strand, within AO353_02535 at 540.654 kb on + strand, within AO353_02535 at 540.654 kb on + strand, within AO353_02535 at 540.654 kb on + strand, within AO353_02535 at 540.654 kb on + strand, within AO353_02535 at 540.655 kb on - strand, within AO353_02535 at 540.655 kb on - strand, within AO353_02535 at 540.655 kb on - strand, within AO353_02535 at 540.655 kb on - strand, within AO353_02535 at 540.810 kb on + strand, within AO353_02535 at 540.811 kb on - strand, within AO353_02535 at 540.811 kb on - strand at 540.811 kb on - strand, within AO353_02535 at 540.811 kb on - strand, within AO353_02535
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 537,351 - AO353_02520 0.72 -1.2 537,351 - AO353_02520 0.72 -1.2 537,351 - AO353_02520 0.72 +0.9 537,375 + AO353_02520 0.75 +0.4 537,375 + AO353_02520 0.75 +1.8 537,558 + -0.6 537,558 + +3.9 537,558 + +0.8 537,559 - -0.6 537,559 - -0.1 537,559 - -0.2 537,559 - -0.4 537,559 - -2.1 537,559 - -0.6 537,559 - +1.0 537,626 + -2.7 537,646 + -2.7 537,647 - -0.6 537,656 + +0.3 537,656 + -2.9 537,656 + -1.4 537,656 + -1.2 537,658 + -2.1 537,658 + -1.7 537,658 + +1.3 537,659 - +0.6 537,659 - -1.4 537,659 - +0.0 537,659 - +3.1 537,698 + -0.8 537,698 + +0.4 537,698 + +1.8 537,699 - +0.2 537,699 - +1.0 537,701 + +1.5 537,701 + -2.3 537,701 + +1.0 537,701 + +2.0 537,701 + -1.6 537,701 + -2.5 537,702 - +1.5 537,702 - -2.2 537,702 - -2.6 537,702 - -2.8 537,702 - +2.2 537,702 - -0.2 537,802 + AO353_02525 0.20 -0.7 537,802 + AO353_02525 0.20 -1.9 537,803 - AO353_02525 0.20 -1.0 537,803 - AO353_02525 0.20 +0.3 537,803 - AO353_02525 0.20 +0.3 537,803 - AO353_02525 0.20 +0.5 537,803 - AO353_02525 0.20 -2.3 537,804 + AO353_02525 0.20 +1.1 537,804 + AO353_02525 0.20 +1.4 537,805 - AO353_02525 0.21 -1.6 537,805 - AO353_02525 0.21 -0.3 537,858 - AO353_02525 0.37 -1.8 537,858 - AO353_02525 0.37 +0.2 537,914 - AO353_02525 0.55 -0.2 537,914 - AO353_02525 0.55 -2.5 538,057 + +1.9 538,057 + +0.3 538,057 + +0.1 538,058 - +1.0 538,058 - +1.8 538,088 - -0.8 538,093 + -0.9 538,093 + -3.7 538,093 + +1.6 538,093 + -2.4 538,093 + +0.9 538,094 - +1.4 538,094 - +0.5 538,100 + -2.6 538,101 - +0.8 538,101 - +0.9 538,109 - -1.2 538,152 + +0.0 538,152 + -0.1 538,152 + +0.0 538,152 + +0.0 538,153 - -2.1 538,153 - -0.1 538,153 - -1.2 538,153 - +0.8 538,156 + -1.9 538,156 + -1.8 538,156 + +1.0 538,157 - -1.9 538,449 + AO353_02530 0.16 +0.5 538,449 + AO353_02530 0.16 -4.0 538,450 - AO353_02530 0.16 +2.6 538,558 + AO353_02530 0.22 -1.6 538,559 - AO353_02530 0.22 +0.6 538,559 - +0.8 538,559 - AO353_02530 0.22 -2.3 538,559 - AO353_02530 0.22 -2.6 538,559 - AO353_02530 0.22 +1.5 538,559 - AO353_02530 0.22 +1.5 538,559 - AO353_02530 0.22 +1.9 538,560 + AO353_02530 0.22 -1.2 538,560 + AO353_02530 0.22 -2.2 538,560 + AO353_02530 0.22 +1.4 538,560 + AO353_02530 0.22 +0.5 538,560 + AO353_02530 0.22 +0.5 538,560 + AO353_02530 0.22 +0.3 538,560 + AO353_02530 0.22 -1.6 538,560 + AO353_02530 0.22 -2.3 538,561 - AO353_02530 0.22 +0.4 538,561 - AO353_02530 0.22 +0.2 538,561 - AO353_02530 0.22 +0.8 538,561 - AO353_02530 0.22 +2.4 538,561 - AO353_02530 0.22 -1.4 538,561 - AO353_02530 0.22 +1.2 538,561 - AO353_02530 0.22 -2.4 538,561 - AO353_02530 0.22 +2.1 538,561 - AO353_02530 0.22 -2.3 538,561 - AO353_02530 0.22 +0.2 538,569 + AO353_02530 0.22 -1.6 538,569 + AO353_02530 0.22 +0.4 538,569 + AO353_02530 0.22 -2.9 538,569 + AO353_02530 0.22 +1.5 538,569 + AO353_02530 0.22 -1.4 538,569 + AO353_02530 0.22 -1.8 538,569 + AO353_02530 0.22 +0.5 538,569 + AO353_02530 0.22 +0.2 538,569 + AO353_02530 0.22 -2.4 538,569 + AO353_02530 0.22 -1.0 538,570 - AO353_02530 0.22 +1.2 538,570 - AO353_02530 0.22 +0.3 538,570 - AO353_02530 0.22 +1.0 538,570 - AO353_02530 0.22 -0.2 538,570 - AO353_02530 0.22 +0.2 538,572 + AO353_02530 0.22 -2.2 538,572 + AO353_02530 0.22 -1.0 538,572 + AO353_02530 0.22 +0.3 538,572 + AO353_02530 0.22 +0.6 538,572 + AO353_02530 0.22 -0.4 538,572 + AO353_02530 0.22 -2.4 538,572 + AO353_02530 0.22 -2.6 538,572 + AO353_02530 0.22 -1.7 538,572 + AO353_02530 0.22 -0.6 538,572 + AO353_02530 0.22 -1.6 538,573 - AO353_02530 0.23 +1.3 538,573 - AO353_02530 0.23 -2.0 538,573 - AO353_02530 0.23 -0.5 538,704 + AO353_02530 0.29 +0.2 538,704 + +1.8 538,704 + AO353_02530 0.29 -3.0 538,705 - AO353_02530 0.30 +0.6 538,770 + AO353_02530 0.33 +0.3 538,771 - AO353_02530 0.33 +1.1 538,771 - AO353_02530 0.33 -2.2 538,771 - AO353_02530 0.33 -2.3 538,771 - AO353_02530 0.33 -1.4 538,771 - AO353_02530 0.33 -2.0 538,771 - AO353_02530 0.33 -2.9 538,771 - AO353_02530 0.33 +1.4 538,771 - AO353_02530 0.33 +0.8 538,778 + AO353_02530 0.33 -1.6 538,778 + AO353_02530 0.33 -0.6 538,778 + AO353_02530 0.33 +0.9 538,778 + AO353_02530 0.33 -0.6 538,779 - AO353_02530 0.33 +0.8 538,779 - AO353_02530 0.33 -1.7 538,779 - AO353_02530 0.33 -2.2 538,779 - -0.6 538,779 - AO353_02530 0.33 -1.2 538,779 - AO353_02530 0.33 +0.7 538,956 + AO353_02530 0.43 +1.0 538,957 - AO353_02530 0.43 +2.0 538,957 - AO353_02530 0.43 -1.4 538,957 - AO353_02530 0.43 +0.2 538,971 + AO353_02530 0.44 -0.3 538,971 + AO353_02530 0.44 -1.5 539,289 + AO353_02530 0.61 -2.1 539,290 - AO353_02530 0.61 -0.8 539,290 - AO353_02530 0.61 -0.8 539,290 - AO353_02530 0.61 +1.3 539,396 - AO353_02530 0.66 -2.4 539,396 - AO353_02530 0.66 +0.3 539,396 - AO353_02530 0.66 -0.7 539,411 + AO353_02530 0.67 +0.4 539,411 + AO353_02530 0.67 +1.2 539,411 + AO353_02530 0.67 +0.4 539,411 + AO353_02530 0.67 +1.2 539,412 - AO353_02530 0.67 -0.3 539,412 - AO353_02530 0.67 -0.7 539,412 - AO353_02530 0.67 -0.8 539,461 - AO353_02530 0.70 +1.4 539,509 - AO353_02530 0.72 +1.1 539,637 + AO353_02530 0.79 -0.8 539,637 + AO353_02530 0.79 +0.3 539,638 - AO353_02530 0.79 -2.3 539,638 - AO353_02530 0.79 -1.0 539,638 - AO353_02530 0.79 -1.4 539,901 + -0.3 539,902 - +1.4 539,966 - -2.4 540,057 + +0.1 540,057 + +1.8 540,057 + +1.6 540,058 - +0.9 540,058 - +2.6 540,058 - +1.2 540,058 - +1.3 540,070 + +2.8 540,093 + -0.7 540,093 + +1.2 540,093 + -2.1 540,094 - -1.9 540,094 - +0.4 540,094 - -2.9 540,094 - +0.3 540,097 + +2.1 540,097 + -2.1 540,098 - +3.5 540,098 - +0.3 540,111 + +1.9 540,111 + -2.1 540,112 - +1.3 540,112 - +1.1 540,112 - -0.3 540,120 - +1.4 540,288 + AO353_02535 0.15 +1.8 540,307 + AO353_02535 0.17 +0.3 540,307 + AO353_02535 0.17 +0.8 540,307 + AO353_02535 0.17 -2.9 540,307 + AO353_02535 0.17 +0.6 540,307 + AO353_02535 0.17 -0.1 540,308 - AO353_02535 0.17 -1.7 540,308 - AO353_02535 0.17 +1.8 540,308 - AO353_02535 0.17 -1.2 540,308 - AO353_02535 0.17 -2.8 540,308 - AO353_02535 0.17 -1.8 540,308 - AO353_02535 0.17 -0.0 540,409 + AO353_02535 0.26 +0.1 540,409 + AO353_02535 0.26 +3.0 540,409 + AO353_02535 0.26 -1.8 540,409 + AO353_02535 0.26 +0.4 540,409 + AO353_02535 0.26 +0.8 540,409 + AO353_02535 0.26 -0.0 540,409 + AO353_02535 0.26 -0.5 540,409 + AO353_02535 0.26 -2.3 540,410 - AO353_02535 0.26 -0.2 540,410 - AO353_02535 0.26 -0.7 540,410 - AO353_02535 0.26 -1.7 540,410 - AO353_02535 0.26 -2.4 540,410 - AO353_02535 0.26 -2.6 540,592 + AO353_02535 0.42 +1.5 540,599 + AO353_02535 0.42 -1.6 540,599 + +0.2 540,599 + AO353_02535 0.42 +1.0 540,600 - AO353_02535 0.43 -1.7 540,654 + AO353_02535 0.47 +0.2 540,654 + AO353_02535 0.47 -2.9 540,654 + AO353_02535 0.47 +1.3 540,654 + AO353_02535 0.47 -0.6 540,654 + AO353_02535 0.47 -2.9 540,654 + AO353_02535 0.47 +1.8 540,654 + AO353_02535 0.47 +0.1 540,654 + AO353_02535 0.47 +0.9 540,654 + AO353_02535 0.47 -1.4 540,654 + AO353_02535 0.47 +0.0 540,655 - AO353_02535 0.47 -0.8 540,655 - AO353_02535 0.47 +1.3 540,655 - AO353_02535 0.47 +0.7 540,655 - AO353_02535 0.47 +0.3 540,810 + AO353_02535 0.61 +1.6 540,811 - AO353_02535 0.61 -1.2 540,811 - -1.0 540,811 - AO353_02535 0.61 +1.0 540,811 - AO353_02535 0.61 +1.3
Or see this region's nucleotide sequence