Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_01185 and AO353_01190 are separated by 211 nucleotides AO353_01190 and AO353_01195 are separated by 137 nucleotides AO353_01195 and AO353_01200 are separated by 41 nucleotides AO353_01200 and AO353_01205 are separated by 167 nucleotides
AO353_01185: AO353_01185 - superfamily II DNA/RNA helicase, SNF2 family protein, at 262,826 to 263,167
_01185
AO353_01190: AO353_01190 - hypothetical protein, at 263,379 to 263,615
_01190
AO353_01195: AO353_01195 - hypothetical protein, at 263,753 to 264,358
_01195
AO353_01200: AO353_01200 - hypothetical protein, at 264,400 to 264,654
_01200
AO353_01205: AO353_01205 - translation initiation factor 2, at 264,822 to 265,325
_01205
Position (kb) 263
264
265 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 262.821 kb on + strand at 262.821 kb on + strand at 262.821 kb on + strand at 262.821 kb on + strand at 262.822 kb on - strand at 262.822 kb on - strand at 262.822 kb on - strand at 262.822 kb on - strand at 262.822 kb on - strand at 262.822 kb on - strand at 262.822 kb on - strand at 262.822 kb on - strand at 262.822 kb on - strand at 262.822 kb on - strand at 262.828 kb on - strand at 262.835 kb on + strand at 262.844 kb on + strand at 262.844 kb on + strand at 262.844 kb on + strand at 262.845 kb on - strand at 262.845 kb on - strand at 262.845 kb on - strand at 262.845 kb on - strand at 262.845 kb on - strand at 262.935 kb on + strand, within AO353_01185 at 262.935 kb on + strand, within AO353_01185 at 262.935 kb on + strand, within AO353_01185 at 262.935 kb on + strand, within AO353_01185 at 262.935 kb on + strand, within AO353_01185 at 262.935 kb on + strand, within AO353_01185 at 262.935 kb on + strand, within AO353_01185 at 262.935 kb on + strand, within AO353_01185 at 262.936 kb on - strand, within AO353_01185 at 262.936 kb on - strand, within AO353_01185 at 262.944 kb on + strand, within AO353_01185 at 262.944 kb on + strand, within AO353_01185 at 262.944 kb on + strand, within AO353_01185 at 262.944 kb on + strand, within AO353_01185 at 262.944 kb on + strand, within AO353_01185 at 262.944 kb on + strand, within AO353_01185 at 262.944 kb on + strand, within AO353_01185 at 262.945 kb on - strand, within AO353_01185 at 262.945 kb on - strand, within AO353_01185 at 262.945 kb on - strand, within AO353_01185 at 262.945 kb on - strand, within AO353_01185 at 262.945 kb on - strand, within AO353_01185 at 263.070 kb on + strand, within AO353_01185 at 263.070 kb on + strand, within AO353_01185 at 263.070 kb on + strand, within AO353_01185 at 263.071 kb on - strand, within AO353_01185 at 263.154 kb on + strand at 263.155 kb on - strand at 263.194 kb on - strand at 263.274 kb on + strand at 263.274 kb on + strand at 263.274 kb on + strand at 263.275 kb on - strand at 263.275 kb on - strand at 263.275 kb on - strand at 263.295 kb on - strand at 263.327 kb on - strand at 263.380 kb on + strand at 263.589 kb on + strand, within AO353_01190 at 263.589 kb on + strand, within AO353_01190 at 263.589 kb on + strand, within AO353_01190 at 263.590 kb on - strand, within AO353_01190 at 263.630 kb on + strand at 263.631 kb on - strand at 263.631 kb on - strand at 263.631 kb on - strand at 263.649 kb on + strand at 263.649 kb on + strand at 263.649 kb on + strand at 263.649 kb on + strand at 263.649 kb on + strand at 263.650 kb on - strand at 263.650 kb on - strand at 263.650 kb on - strand at 263.650 kb on - strand at 263.650 kb on - strand at 263.706 kb on + strand at 263.707 kb on - strand at 263.707 kb on - strand at 263.754 kb on + strand at 263.754 kb on + strand at 263.754 kb on + strand at 263.754 kb on + strand at 263.754 kb on + strand at 263.754 kb on + strand at 263.754 kb on + strand at 263.754 kb on + strand at 263.755 kb on - strand at 263.755 kb on - strand at 263.755 kb on - strand at 263.755 kb on - strand at 263.755 kb on - strand at 263.755 kb on - strand at 263.755 kb on - strand at 263.788 kb on + strand at 263.788 kb on + strand at 263.788 kb on + strand at 263.789 kb on - strand at 263.789 kb on - strand at 263.789 kb on - strand at 263.840 kb on - strand, within AO353_01195 at 263.865 kb on + strand, within AO353_01195 at 263.866 kb on - strand, within AO353_01195 at 263.866 kb on - strand, within AO353_01195 at 263.866 kb on - strand, within AO353_01195 at 263.866 kb on - strand, within AO353_01195 at 263.958 kb on + strand, within AO353_01195 at 263.958 kb on + strand, within AO353_01195 at 263.958 kb on + strand at 263.959 kb on - strand, within AO353_01195 at 263.959 kb on - strand, within AO353_01195 at 263.959 kb on - strand, within AO353_01195 at 263.959 kb on - strand, within AO353_01195 at 263.959 kb on - strand, within AO353_01195 at 263.959 kb on - strand, within AO353_01195 at 263.980 kb on + strand, within AO353_01195 at 263.980 kb on + strand, within AO353_01195 at 263.981 kb on - strand, within AO353_01195 at 263.981 kb on - strand, within AO353_01195 at 264.013 kb on + strand, within AO353_01195 at 264.014 kb on - strand, within AO353_01195 at 264.014 kb on - strand, within AO353_01195 at 264.072 kb on + strand, within AO353_01195 at 264.072 kb on + strand, within AO353_01195 at 264.072 kb on + strand, within AO353_01195 at 264.073 kb on - strand, within AO353_01195 at 264.073 kb on - strand, within AO353_01195 at 264.073 kb on - strand, within AO353_01195 at 264.073 kb on - strand, within AO353_01195 at 264.210 kb on + strand, within AO353_01195 at 264.219 kb on + strand, within AO353_01195 at 264.220 kb on - strand, within AO353_01195 at 264.220 kb on - strand, within AO353_01195 at 264.220 kb on - strand, within AO353_01195 at 264.340 kb on + strand at 264.340 kb on + strand at 264.358 kb on + strand at 264.359 kb on - strand at 264.359 kb on - strand at 264.364 kb on + strand at 264.364 kb on + strand at 264.364 kb on + strand at 264.364 kb on + strand at 264.365 kb on - strand at 264.365 kb on - strand at 264.365 kb on - strand at 264.365 kb on - strand at 264.372 kb on - strand at 264.372 kb on - strand at 264.372 kb on - strand at 264.372 kb on - strand at 264.401 kb on + strand at 264.402 kb on - strand at 264.402 kb on - strand at 264.402 kb on - strand at 264.525 kb on + strand, within AO353_01200 at 264.526 kb on - strand, within AO353_01200 at 264.679 kb on - strand at 264.700 kb on + strand at 264.700 kb on + strand at 264.701 kb on - strand at 264.701 kb on - strand at 264.701 kb on - strand at 264.701 kb on - strand at 264.701 kb on - strand at 264.739 kb on + strand at 264.768 kb on + strand at 264.830 kb on + strand at 264.830 kb on + strand at 264.830 kb on + strand at 264.830 kb on + strand at 264.990 kb on + strand, within AO353_01205 at 265.014 kb on + strand, within AO353_01205 at 265.014 kb on + strand, within AO353_01205 at 265.014 kb on + strand, within AO353_01205 at 265.014 kb on + strand, within AO353_01205 at 265.014 kb on + strand, within AO353_01205 at 265.014 kb on + strand, within AO353_01205 at 265.015 kb on - strand, within AO353_01205 at 265.015 kb on - strand, within AO353_01205 at 265.015 kb on - strand, within AO353_01205 at 265.015 kb on - strand, within AO353_01205 at 265.015 kb on - strand, within AO353_01205 at 265.015 kb on - strand, within AO353_01205 at 265.069 kb on + strand, within AO353_01205 at 265.069 kb on + strand, within AO353_01205 at 265.069 kb on + strand, within AO353_01205 at 265.069 kb on + strand, within AO353_01205 at 265.069 kb on + strand, within AO353_01205 at 265.070 kb on - strand, within AO353_01205 at 265.070 kb on - strand, within AO353_01205 at 265.070 kb on - strand, within AO353_01205 at 265.070 kb on - strand, within AO353_01205 at 265.070 kb on - strand at 265.150 kb on - strand, within AO353_01205 at 265.150 kb on - strand, within AO353_01205 at 265.150 kb on - strand, within AO353_01205 at 265.173 kb on + strand, within AO353_01205 at 265.173 kb on + strand, within AO353_01205 at 265.173 kb on + strand, within AO353_01205 at 265.173 kb on + strand, within AO353_01205 at 265.173 kb on + strand, within AO353_01205 at 265.173 kb on + strand, within AO353_01205 at 265.174 kb on - strand, within AO353_01205 at 265.174 kb on - strand, within AO353_01205 at 265.174 kb on - strand at 265.174 kb on - strand, within AO353_01205 at 265.174 kb on - strand, within AO353_01205 at 265.174 kb on - strand, within AO353_01205 at 265.174 kb on - strand, within AO353_01205 at 265.174 kb on - strand, within AO353_01205 at 265.174 kb on - strand, within AO353_01205 at 265.332 kb on + strand at 265.332 kb on + strand at 265.332 kb on + strand at 265.333 kb on - strand at 265.333 kb on - strand at 265.333 kb on - strand at 265.333 kb on - strand at 265.333 kb on - strand at 265.333 kb on - strand at 265.333 kb on - strand at 265.356 kb on + strand at 265.356 kb on + strand at 265.356 kb on + strand at 265.357 kb on - strand at 265.357 kb on - strand at 265.357 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 262,821 + -0.7 262,821 + -1.2 262,821 + +0.4 262,821 + +0.1 262,822 - -0.9 262,822 - -1.5 262,822 - -0.1 262,822 - +0.1 262,822 - +0.9 262,822 - -0.9 262,822 - +0.6 262,822 - +3.4 262,822 - -1.5 262,822 - -3.4 262,828 - +0.3 262,835 + +0.3 262,844 + +0.6 262,844 + -0.0 262,844 + -1.4 262,845 - -0.7 262,845 - +0.6 262,845 - +1.8 262,845 - +2.4 262,845 - -0.1 262,935 + AO353_01185 0.32 +0.1 262,935 + AO353_01185 0.32 +1.1 262,935 + AO353_01185 0.32 -0.3 262,935 + AO353_01185 0.32 +2.2 262,935 + AO353_01185 0.32 +1.2 262,935 + AO353_01185 0.32 +1.0 262,935 + AO353_01185 0.32 +0.4 262,935 + AO353_01185 0.32 -1.0 262,936 - AO353_01185 0.32 -1.2 262,936 - AO353_01185 0.32 -0.1 262,944 + AO353_01185 0.35 -0.1 262,944 + AO353_01185 0.35 +2.0 262,944 + AO353_01185 0.35 +0.0 262,944 + AO353_01185 0.35 +0.1 262,944 + AO353_01185 0.35 -0.4 262,944 + AO353_01185 0.35 -1.5 262,944 + AO353_01185 0.35 -1.4 262,945 - AO353_01185 0.35 -1.4 262,945 - AO353_01185 0.35 +2.0 262,945 - AO353_01185 0.35 +0.1 262,945 - AO353_01185 0.35 -1.8 262,945 - AO353_01185 0.35 -1.2 263,070 + AO353_01185 0.71 +2.4 263,070 + AO353_01185 0.71 +0.5 263,070 + AO353_01185 0.71 +1.2 263,071 - AO353_01185 0.72 -0.6 263,154 + -2.2 263,155 - +0.6 263,194 - -0.6 263,274 + -0.7 263,274 + -2.4 263,274 + -0.4 263,275 - +2.1 263,275 - +0.2 263,275 - +1.6 263,295 - +2.6 263,327 - -0.7 263,380 + -3.1 263,589 + AO353_01190 0.89 -1.8 263,589 + AO353_01190 0.89 +0.2 263,589 + AO353_01190 0.89 +1.3 263,590 - AO353_01190 0.89 -2.4 263,630 + +1.9 263,631 - -1.0 263,631 - -2.1 263,631 - -0.2 263,649 + -0.6 263,649 + -0.9 263,649 + -1.5 263,649 + -2.7 263,649 + +1.9 263,650 - +0.7 263,650 - +0.1 263,650 - -0.5 263,650 - -1.2 263,650 - +3.3 263,706 + -2.4 263,707 - -0.2 263,707 - -0.2 263,754 + +0.8 263,754 + -2.4 263,754 + +0.6 263,754 + -1.5 263,754 + +1.1 263,754 + -2.2 263,754 + +2.9 263,754 + -3.0 263,755 - -0.5 263,755 - +1.4 263,755 - +0.6 263,755 - +1.6 263,755 - -1.3 263,755 - -1.4 263,755 - +0.5 263,788 + -0.2 263,788 + +0.2 263,788 + -0.6 263,789 - +1.4 263,789 - -1.8 263,789 - +2.1 263,840 - AO353_01195 0.14 -0.7 263,865 + AO353_01195 0.18 -1.9 263,866 - AO353_01195 0.19 -1.5 263,866 - AO353_01195 0.19 +1.0 263,866 - AO353_01195 0.19 -1.2 263,866 - AO353_01195 0.19 -0.3 263,958 + AO353_01195 0.34 -0.7 263,958 + AO353_01195 0.34 -0.6 263,958 + -0.9 263,959 - AO353_01195 0.34 -0.4 263,959 - AO353_01195 0.34 +0.1 263,959 - AO353_01195 0.34 -1.5 263,959 - AO353_01195 0.34 +0.3 263,959 - AO353_01195 0.34 +2.0 263,959 - AO353_01195 0.34 +2.6 263,980 + AO353_01195 0.37 +0.2 263,980 + AO353_01195 0.37 -0.6 263,981 - AO353_01195 0.38 +0.6 263,981 - AO353_01195 0.38 -1.8 264,013 + AO353_01195 0.43 +0.2 264,014 - AO353_01195 0.43 -0.4 264,014 - AO353_01195 0.43 +2.1 264,072 + AO353_01195 0.53 -2.3 264,072 + AO353_01195 0.53 +0.1 264,072 + AO353_01195 0.53 -1.3 264,073 - AO353_01195 0.53 -2.1 264,073 - AO353_01195 0.53 -0.4 264,073 - AO353_01195 0.53 +1.4 264,073 - AO353_01195 0.53 -0.3 264,210 + AO353_01195 0.75 +0.6 264,219 + AO353_01195 0.77 +0.9 264,220 - AO353_01195 0.77 +0.1 264,220 - AO353_01195 0.77 +1.3 264,220 - AO353_01195 0.77 -1.3 264,340 + -0.4 264,340 + +1.9 264,358 + +1.1 264,359 - -1.9 264,359 - +1.6 264,364 + +1.3 264,364 + -0.1 264,364 + +0.4 264,364 + -0.4 264,365 - -1.1 264,365 - -0.9 264,365 - +1.1 264,365 - -1.2 264,372 - +1.9 264,372 - +1.3 264,372 - -0.4 264,372 - +0.3 264,401 + +1.3 264,402 - +2.1 264,402 - +0.2 264,402 - -0.9 264,525 + AO353_01200 0.49 -0.8 264,526 - AO353_01200 0.49 +1.8 264,679 - -0.3 264,700 + -0.7 264,700 + -0.6 264,701 - -0.2 264,701 - -0.5 264,701 - -2.1 264,701 - -1.1 264,701 - +1.4 264,739 + +0.2 264,768 + -1.8 264,830 + +0.6 264,830 + +1.1 264,830 + +1.0 264,830 + -0.9 264,990 + AO353_01205 0.33 -1.5 265,014 + AO353_01205 0.38 +0.1 265,014 + AO353_01205 0.38 -1.4 265,014 + AO353_01205 0.38 +1.5 265,014 + AO353_01205 0.38 +1.1 265,014 + AO353_01205 0.38 -0.1 265,014 + AO353_01205 0.38 +1.1 265,015 - AO353_01205 0.38 -1.5 265,015 - AO353_01205 0.38 -0.5 265,015 - AO353_01205 0.38 -0.0 265,015 - AO353_01205 0.38 +1.7 265,015 - AO353_01205 0.38 +1.9 265,015 - AO353_01205 0.38 -0.1 265,069 + AO353_01205 0.49 -0.2 265,069 + AO353_01205 0.49 -0.4 265,069 + AO353_01205 0.49 +0.8 265,069 + AO353_01205 0.49 -1.5 265,069 + AO353_01205 0.49 +1.6 265,070 - AO353_01205 0.49 +0.6 265,070 - AO353_01205 0.49 -0.5 265,070 - AO353_01205 0.49 -1.2 265,070 - AO353_01205 0.49 -1.4 265,070 - +2.2 265,150 - AO353_01205 0.65 -0.4 265,150 - AO353_01205 0.65 +1.6 265,150 - AO353_01205 0.65 +0.3 265,173 + AO353_01205 0.70 +0.1 265,173 + AO353_01205 0.70 +1.3 265,173 + AO353_01205 0.70 +0.5 265,173 + AO353_01205 0.70 -0.5 265,173 + AO353_01205 0.70 +0.5 265,173 + AO353_01205 0.70 +0.1 265,174 - AO353_01205 0.70 -0.1 265,174 - AO353_01205 0.70 -0.4 265,174 - +2.3 265,174 - AO353_01205 0.70 +0.2 265,174 - AO353_01205 0.70 +0.6 265,174 - AO353_01205 0.70 -2.8 265,174 - AO353_01205 0.70 -0.7 265,174 - AO353_01205 0.70 -1.9 265,174 - AO353_01205 0.70 +0.1 265,332 + -1.5 265,332 + +0.4 265,332 + -0.1 265,333 - +1.3 265,333 - +0.3 265,333 - -2.2 265,333 - +0.2 265,333 - +1.3 265,333 - +2.2 265,333 - -1.7 265,356 + +0.8 265,356 + -2.1 265,356 + +2.4 265,357 - +0.8 265,357 - +0.1 265,357 - -1.9
Or see this region's nucleotide sequence