Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2093 and Shew_2094 overlap by 8 nucleotides Shew_2094 and Shew_2095 overlap by 14 nucleotides Shew_2095 and Shew_2096 are separated by 2 nucleotides
Shew_2093: Shew_2093 - thiamine biosynthesis protein ThiS (RefSeq), at 2,420,124 to 2,420,333
_2093
Shew_2094: Shew_2094 - UBA/THIF-type NAD/FAD binding protein (RefSeq), at 2,420,326 to 2,421,174
_2094
Shew_2095: Shew_2095 - phosphomethylpyrimidine kinase (RefSeq), at 2,421,161 to 2,422,906
_2095
Shew_2096: Shew_2096 - thiamine biosynthesis protein ThiC (RefSeq), at 2,422,909 to 2,424,960
_2096
Position (kb)
2421
2422
2423 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2420.359 kb on + strand at 2420.367 kb on - strand at 2420.726 kb on + strand, within Shew_2094 at 2420.810 kb on + strand, within Shew_2094 at 2420.918 kb on + strand, within Shew_2094 at 2421.046 kb on - strand, within Shew_2094 at 2421.179 kb on - strand at 2421.413 kb on - strand, within Shew_2095 at 2421.438 kb on + strand, within Shew_2095 at 2421.899 kb on + strand, within Shew_2095 at 2422.252 kb on - strand, within Shew_2095 at 2422.306 kb on + strand, within Shew_2095 at 2422.319 kb on - strand, within Shew_2095 at 2422.388 kb on - strand, within Shew_2095 at 2422.398 kb on - strand, within Shew_2095 at 2422.408 kb on + strand, within Shew_2095 at 2422.593 kb on + strand, within Shew_2095 at 2422.663 kb on + strand, within Shew_2095 at 2422.663 kb on + strand, within Shew_2095 at 2422.663 kb on + strand, within Shew_2095 at 2423.079 kb on - strand at 2423.192 kb on - strand, within Shew_2096 at 2423.192 kb on - strand, within Shew_2096
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,420,359 + +2.5 2,420,367 - +1.0 2,420,726 + Shew_2094 0.47 +1.7 2,420,810 + Shew_2094 0.57 +0.2 2,420,918 + Shew_2094 0.70 +0.7 2,421,046 - Shew_2094 0.85 -0.1 2,421,179 - +0.3 2,421,413 - Shew_2095 0.14 +1.5 2,421,438 + Shew_2095 0.16 +0.7 2,421,899 + Shew_2095 0.42 -0.1 2,422,252 - Shew_2095 0.62 -0.4 2,422,306 + Shew_2095 0.66 -2.2 2,422,319 - Shew_2095 0.66 +1.7 2,422,388 - Shew_2095 0.70 +0.4 2,422,398 - Shew_2095 0.71 -0.2 2,422,408 + Shew_2095 0.71 -0.2 2,422,593 + Shew_2095 0.82 +0.6 2,422,663 + Shew_2095 0.86 +0.9 2,422,663 + Shew_2095 0.86 -0.2 2,422,663 + Shew_2095 0.86 +0.2 2,423,079 - +0.0 2,423,192 - Shew_2096 0.14 +0.1 2,423,192 - Shew_2096 0.14 +0.2
Or see this region's nucleotide sequence