Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0013 and Shew_0014 are separated by 302 nucleotides Shew_0014 and Shew_0015 are separated by 165 nucleotides Shew_0015 and Shew_0016 are separated by 133 nucleotides
Shew_0013: Shew_0013 - amidohydrolase (RefSeq), at 14,072 to 17,275
_0013
Shew_0014: Shew_0014 - hypothetical protein (RefSeq), at 17,578 to 18,018
_0014
Shew_0015: Shew_0015 - SirA family protein (RefSeq), at 18,184 to 18,429
_0015
Shew_0016: Shew_0016 - ybaK/ebsC protein (RefSeq), at 18,563 to 19,027
_0016
Shew_0017: Shew_0017 - GCN5-related N-acetyltransferase (RefSeq), at 19,011 to 19,499
_0017
Position (kb)
17
18
19 Strain fitness (log2 ratio)
-1
0
1 at 16.616 kb on + strand, within Shew_0013 at 16.625 kb on - strand, within Shew_0013 at 16.641 kb on - strand, within Shew_0013 at 16.696 kb on + strand, within Shew_0013 at 16.765 kb on + strand, within Shew_0013 at 16.779 kb on - strand, within Shew_0013 at 17.000 kb on + strand at 17.118 kb on + strand at 17.126 kb on - strand at 17.236 kb on - strand at 17.731 kb on - strand, within Shew_0014 at 17.744 kb on - strand, within Shew_0014 at 18.105 kb on + strand at 18.144 kb on - strand at 18.181 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 16,616 + Shew_0013 0.79 +0.6 16,625 - Shew_0013 0.80 -0.1 16,641 - Shew_0013 0.80 +0.1 16,696 + Shew_0013 0.82 +0.3 16,765 + Shew_0013 0.84 -0.5 16,779 - Shew_0013 0.84 +0.7 17,000 + +0.1 17,118 + -0.5 17,126 - +0.7 17,236 - +0.1 17,731 - Shew_0014 0.35 -0.1 17,744 - Shew_0014 0.38 -1.3 18,105 + +0.8 18,144 - +0.1 18,181 + +0.4
Or see this region's nucleotide sequence