Experiment: reconstituted MRS with Sialyllacto-N-tetraose a 5.0 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13095 and BBR_RS13100 are separated by 255 nucleotides BBR_RS13100 and BBR_RS13105 are separated by 162 nucleotides
BBR_RS13095: BBR_RS13095 - beta-galactosidase, at 711,197 to 713,272
_RS13095
BBR_RS13100: BBR_RS13100 - sugar ABC transporter substrate-binding protein, at 713,528 to 714,874
_RS13100
BBR_RS13105: BBR_RS13105 - sugar ABC transporter substrate-binding protein, at 715,037 to 716,458
_RS13105
Position (kb) 713
714
715 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 712.558 kb on + strand, within BBR_RS13095 at 712.588 kb on + strand, within BBR_RS13095 at 712.610 kb on + strand, within BBR_RS13095 at 712.641 kb on - strand, within BBR_RS13095 at 712.655 kb on + strand, within BBR_RS13095 at 712.668 kb on + strand, within BBR_RS13095 at 712.678 kb on + strand, within BBR_RS13095 at 712.687 kb on + strand, within BBR_RS13095 at 712.687 kb on + strand, within BBR_RS13095 at 712.714 kb on + strand, within BBR_RS13095 at 712.723 kb on - strand, within BBR_RS13095 at 712.723 kb on - strand, within BBR_RS13095 at 712.743 kb on + strand, within BBR_RS13095 at 712.800 kb on - strand, within BBR_RS13095 at 712.811 kb on + strand, within BBR_RS13095 at 712.811 kb on + strand, within BBR_RS13095 at 712.821 kb on + strand, within BBR_RS13095 at 712.830 kb on - strand, within BBR_RS13095 at 712.836 kb on + strand, within BBR_RS13095 at 712.839 kb on + strand, within BBR_RS13095 at 712.873 kb on - strand, within BBR_RS13095 at 712.880 kb on - strand, within BBR_RS13095 at 712.887 kb on + strand, within BBR_RS13095 at 712.897 kb on + strand, within BBR_RS13095 at 712.897 kb on + strand, within BBR_RS13095 at 712.906 kb on + strand, within BBR_RS13095 at 712.906 kb on + strand, within BBR_RS13095 at 713.011 kb on + strand, within BBR_RS13095 at 713.014 kb on - strand, within BBR_RS13095 at 713.020 kb on - strand, within BBR_RS13095 at 713.023 kb on + strand, within BBR_RS13095 at 713.038 kb on + strand, within BBR_RS13095 at 713.112 kb on + strand at 713.112 kb on + strand at 713.117 kb on + strand at 713.130 kb on - strand at 713.138 kb on - strand at 713.199 kb on + strand at 713.217 kb on - strand at 713.266 kb on + strand at 713.266 kb on + strand at 713.266 kb on + strand at 713.266 kb on + strand at 713.274 kb on - strand at 713.274 kb on - strand at 713.281 kb on + strand at 713.284 kb on - strand at 713.425 kb on - strand at 713.558 kb on + strand at 713.558 kb on + strand at 713.566 kb on - strand at 713.584 kb on + strand at 713.641 kb on - strand at 713.713 kb on + strand, within BBR_RS13100 at 713.735 kb on - strand, within BBR_RS13100 at 713.769 kb on - strand, within BBR_RS13100 at 713.771 kb on + strand, within BBR_RS13100 at 713.779 kb on - strand, within BBR_RS13100 at 713.789 kb on - strand, within BBR_RS13100 at 713.795 kb on + strand, within BBR_RS13100 at 713.827 kb on + strand, within BBR_RS13100 at 713.827 kb on + strand, within BBR_RS13100 at 713.835 kb on - strand, within BBR_RS13100 at 713.837 kb on + strand, within BBR_RS13100 at 713.902 kb on + strand, within BBR_RS13100 at 713.910 kb on + strand, within BBR_RS13100 at 713.910 kb on + strand, within BBR_RS13100 at 713.910 kb on + strand, within BBR_RS13100 at 713.918 kb on - strand, within BBR_RS13100 at 713.918 kb on - strand, within BBR_RS13100 at 713.925 kb on + strand, within BBR_RS13100 at 713.933 kb on - strand, within BBR_RS13100 at 713.933 kb on - strand, within BBR_RS13100 at 713.948 kb on + strand, within BBR_RS13100 at 713.948 kb on + strand, within BBR_RS13100 at 713.948 kb on + strand, within BBR_RS13100 at 713.948 kb on + strand, within BBR_RS13100 at 713.955 kb on - strand, within BBR_RS13100 at 713.955 kb on - strand, within BBR_RS13100 at 713.955 kb on - strand, within BBR_RS13100 at 713.955 kb on - strand, within BBR_RS13100 at 713.984 kb on + strand, within BBR_RS13100 at 714.034 kb on + strand, within BBR_RS13100 at 714.034 kb on + strand, within BBR_RS13100 at 714.060 kb on - strand, within BBR_RS13100 at 714.060 kb on - strand, within BBR_RS13100 at 714.112 kb on - strand, within BBR_RS13100 at 714.167 kb on + strand, within BBR_RS13100 at 714.186 kb on + strand, within BBR_RS13100 at 714.186 kb on + strand, within BBR_RS13100 at 714.206 kb on - strand, within BBR_RS13100 at 714.232 kb on - strand, within BBR_RS13100 at 714.242 kb on - strand, within BBR_RS13100 at 714.267 kb on - strand, within BBR_RS13100 at 714.343 kb on + strand, within BBR_RS13100 at 714.351 kb on - strand, within BBR_RS13100 at 714.363 kb on + strand, within BBR_RS13100 at 714.454 kb on + strand, within BBR_RS13100 at 714.481 kb on - strand, within BBR_RS13100 at 714.504 kb on + strand, within BBR_RS13100 at 714.504 kb on - strand, within BBR_RS13100 at 714.542 kb on + strand, within BBR_RS13100 at 714.555 kb on - strand, within BBR_RS13100 at 714.555 kb on - strand, within BBR_RS13100 at 714.616 kb on + strand, within BBR_RS13100 at 714.624 kb on - strand, within BBR_RS13100 at 714.624 kb on - strand, within BBR_RS13100 at 714.624 kb on - strand, within BBR_RS13100 at 714.641 kb on + strand, within BBR_RS13100 at 714.641 kb on + strand, within BBR_RS13100 at 714.655 kb on + strand, within BBR_RS13100 at 714.688 kb on + strand, within BBR_RS13100 at 714.706 kb on - strand, within BBR_RS13100 at 714.725 kb on + strand, within BBR_RS13100 at 714.748 kb on - strand at 714.748 kb on - strand at 714.748 kb on - strand at 714.748 kb on - strand at 714.768 kb on + strand at 714.778 kb on + strand at 714.778 kb on + strand at 714.778 kb on + strand at 714.778 kb on + strand at 714.786 kb on - strand at 714.786 kb on - strand at 714.786 kb on - strand at 714.786 kb on - strand at 714.786 kb on - strand at 714.786 kb on - strand at 714.786 kb on - strand at 714.851 kb on + strand at 714.859 kb on - strand at 714.859 kb on - strand at 714.869 kb on + strand at 714.911 kb on - strand at 714.956 kb on - strand at 714.957 kb on + strand at 714.975 kb on + strand at 714.981 kb on + strand at 715.021 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Sialyllacto-N-tetraose a 5.0 mM remove 712,558 + BBR_RS13095 0.66 -1.4 712,588 + BBR_RS13095 0.67 -1.1 712,610 + BBR_RS13095 0.68 -0.6 712,641 - BBR_RS13095 0.70 -1.8 712,655 + BBR_RS13095 0.70 -0.8 712,668 + BBR_RS13095 0.71 -2.1 712,678 + BBR_RS13095 0.71 -2.5 712,687 + BBR_RS13095 0.72 -1.8 712,687 + BBR_RS13095 0.72 -1.2 712,714 + BBR_RS13095 0.73 -1.4 712,723 - BBR_RS13095 0.74 +0.0 712,723 - BBR_RS13095 0.74 -0.9 712,743 + BBR_RS13095 0.74 -1.5 712,800 - BBR_RS13095 0.77 -1.2 712,811 + BBR_RS13095 0.78 -0.8 712,811 + BBR_RS13095 0.78 -0.5 712,821 + BBR_RS13095 0.78 -1.7 712,830 - BBR_RS13095 0.79 -1.5 712,836 + BBR_RS13095 0.79 -1.7 712,839 + BBR_RS13095 0.79 -0.1 712,873 - BBR_RS13095 0.81 -0.6 712,880 - BBR_RS13095 0.81 -1.2 712,887 + BBR_RS13095 0.81 -0.1 712,897 + BBR_RS13095 0.82 -1.0 712,897 + BBR_RS13095 0.82 -2.0 712,906 + BBR_RS13095 0.82 -0.9 712,906 + BBR_RS13095 0.82 -2.6 713,011 + BBR_RS13095 0.87 -1.9 713,014 - BBR_RS13095 0.88 -1.3 713,020 - BBR_RS13095 0.88 -1.0 713,023 + BBR_RS13095 0.88 -2.3 713,038 + BBR_RS13095 0.89 -2.3 713,112 + -2.7 713,112 + -0.5 713,117 + -2.2 713,130 - -2.5 713,138 - -1.8 713,199 + -1.7 713,217 - -3.1 713,266 + +0.0 713,266 + +0.5 713,266 + -0.3 713,266 + -0.1 713,274 - -1.1 713,274 - -0.2 713,281 + -0.2 713,284 - +1.6 713,425 - +0.7 713,558 + -0.4 713,558 + +0.5 713,566 - +0.2 713,584 + -0.7 713,641 - +0.6 713,713 + BBR_RS13100 0.14 -0.7 713,735 - BBR_RS13100 0.15 -0.2 713,769 - BBR_RS13100 0.18 +0.0 713,771 + BBR_RS13100 0.18 -0.2 713,779 - BBR_RS13100 0.19 -0.2 713,789 - BBR_RS13100 0.19 -0.8 713,795 + BBR_RS13100 0.20 +0.2 713,827 + BBR_RS13100 0.22 -0.6 713,827 + BBR_RS13100 0.22 -0.3 713,835 - BBR_RS13100 0.23 +0.0 713,837 + BBR_RS13100 0.23 +0.4 713,902 + BBR_RS13100 0.28 -0.1 713,910 + BBR_RS13100 0.28 +1.5 713,910 + BBR_RS13100 0.28 -0.8 713,910 + BBR_RS13100 0.28 -0.4 713,918 - BBR_RS13100 0.29 -1.0 713,918 - BBR_RS13100 0.29 +0.0 713,925 + BBR_RS13100 0.29 -0.8 713,933 - BBR_RS13100 0.30 -0.4 713,933 - BBR_RS13100 0.30 +0.3 713,948 + BBR_RS13100 0.31 +0.7 713,948 + BBR_RS13100 0.31 +0.5 713,948 + BBR_RS13100 0.31 +0.9 713,948 + BBR_RS13100 0.31 +1.1 713,955 - BBR_RS13100 0.32 +0.6 713,955 - BBR_RS13100 0.32 -0.1 713,955 - BBR_RS13100 0.32 +1.1 713,955 - BBR_RS13100 0.32 +0.3 713,984 + BBR_RS13100 0.34 -0.1 714,034 + BBR_RS13100 0.38 -1.2 714,034 + BBR_RS13100 0.38 +0.2 714,060 - BBR_RS13100 0.39 -0.1 714,060 - BBR_RS13100 0.39 -0.2 714,112 - BBR_RS13100 0.43 +0.4 714,167 + BBR_RS13100 0.47 +0.0 714,186 + BBR_RS13100 0.49 +0.5 714,186 + BBR_RS13100 0.49 -0.2 714,206 - BBR_RS13100 0.50 +0.4 714,232 - BBR_RS13100 0.52 -0.5 714,242 - BBR_RS13100 0.53 -0.1 714,267 - BBR_RS13100 0.55 +1.2 714,343 + BBR_RS13100 0.61 +0.7 714,351 - BBR_RS13100 0.61 -0.1 714,363 + BBR_RS13100 0.62 -0.1 714,454 + BBR_RS13100 0.69 -0.0 714,481 - BBR_RS13100 0.71 -2.5 714,504 + BBR_RS13100 0.72 +0.4 714,504 - BBR_RS13100 0.72 -0.2 714,542 + BBR_RS13100 0.75 +0.7 714,555 - BBR_RS13100 0.76 +0.5 714,555 - BBR_RS13100 0.76 -0.1 714,616 + BBR_RS13100 0.81 +0.6 714,624 - BBR_RS13100 0.81 +0.1 714,624 - BBR_RS13100 0.81 +0.1 714,624 - BBR_RS13100 0.81 +0.7 714,641 + BBR_RS13100 0.83 -0.1 714,641 + BBR_RS13100 0.83 -0.6 714,655 + BBR_RS13100 0.84 -0.4 714,688 + BBR_RS13100 0.86 +0.6 714,706 - BBR_RS13100 0.87 -0.4 714,725 + BBR_RS13100 0.89 -0.8 714,748 - -0.2 714,748 - +0.4 714,748 - -0.1 714,748 - +0.6 714,768 + +0.5 714,778 + +0.4 714,778 + +0.2 714,778 + -0.1 714,778 + -1.1 714,786 - -0.5 714,786 - +1.3 714,786 - +0.7 714,786 - +0.3 714,786 - -0.6 714,786 - +0.6 714,786 - -0.3 714,851 + +0.3 714,859 - -0.3 714,859 - -0.5 714,869 + +0.7 714,911 - +0.2 714,956 - -0.1 714,957 + +1.2 714,975 + +0.4 714,981 + -0.0 715,021 + -0.3
Or see this region's nucleotide sequence