Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3632 and Shew_3633 are separated by 492 nucleotides Shew_3633 and Shew_3634 are separated by 87 nucleotides Shew_3634 and Shew_3635 are separated by 127 nucleotides
Shew_3632: Shew_3632 - sulfatase (RefSeq), at 4,329,404 to 4,331,392
_3632
Shew_3633: Shew_3633 - hypothetical protein (RefSeq), at 4,331,885 to 4,332,103
_3633
Shew_3634: Shew_3634 - RNA-binding S1 domain-containing protein (RefSeq), at 4,332,191 to 4,334,554
_3634
Shew_3635: Shew_3635 - thiamine pyrophosphate binding domain-containing protein (RefSeq), at 4,334,682 to 4,336,493
_3635
Position (kb)
4332
4333
4334
4335 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4331.743 kb on + strand at 4332.446 kb on - strand, within Shew_3634 at 4332.452 kb on - strand, within Shew_3634 at 4332.622 kb on + strand, within Shew_3634 at 4332.630 kb on - strand, within Shew_3634 at 4332.648 kb on + strand, within Shew_3634 at 4332.686 kb on - strand, within Shew_3634 at 4332.868 kb on + strand, within Shew_3634 at 4333.106 kb on - strand, within Shew_3634 at 4333.338 kb on + strand, within Shew_3634 at 4333.551 kb on + strand, within Shew_3634 at 4333.717 kb on + strand, within Shew_3634 at 4334.118 kb on + strand, within Shew_3634 at 4334.387 kb on + strand at 4334.395 kb on - strand at 4334.404 kb on + strand at 4334.556 kb on + strand at 4334.603 kb on - strand at 4334.618 kb on - strand at 4334.880 kb on + strand, within Shew_3635 at 4334.888 kb on - strand, within Shew_3635 at 4334.907 kb on - strand, within Shew_3635 at 4334.940 kb on + strand, within Shew_3635 at 4335.100 kb on + strand, within Shew_3635 at 4335.216 kb on + strand, within Shew_3635 at 4335.417 kb on - strand, within Shew_3635 at 4335.434 kb on - strand, within Shew_3635 at 4335.467 kb on - strand, within Shew_3635
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 4,331,743 + -0.2 4,332,446 - Shew_3634 0.11 -1.8 4,332,452 - Shew_3634 0.11 -1.5 4,332,622 + Shew_3634 0.18 -3.1 4,332,630 - Shew_3634 0.19 -1.1 4,332,648 + Shew_3634 0.19 -1.0 4,332,686 - Shew_3634 0.21 -1.7 4,332,868 + Shew_3634 0.29 -1.4 4,333,106 - Shew_3634 0.39 -1.7 4,333,338 + Shew_3634 0.49 -1.3 4,333,551 + Shew_3634 0.58 -1.6 4,333,717 + Shew_3634 0.65 -0.3 4,334,118 + Shew_3634 0.82 -1.4 4,334,387 + -1.7 4,334,395 - -1.1 4,334,404 + -1.4 4,334,556 + -1.6 4,334,603 - -0.6 4,334,618 - -0.0 4,334,880 + Shew_3635 0.11 +0.2 4,334,888 - Shew_3635 0.11 +0.1 4,334,907 - Shew_3635 0.12 +0.2 4,334,940 + Shew_3635 0.14 +0.4 4,335,100 + Shew_3635 0.23 +0.0 4,335,216 + Shew_3635 0.29 +0.0 4,335,417 - Shew_3635 0.41 +0.2 4,335,434 - Shew_3635 0.42 -0.6 4,335,467 - Shew_3635 0.43 -0.3
Or see this region's nucleotide sequence