Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt moaA and Shew_0088 are separated by 111 nucleotides Shew_0088 and moaC overlap by 8 nucleotides moaC and Shew_0090 are separated by 49 nucleotides Shew_0090 and Shew_0091 are separated by 1 nucleotides
Shew_0087: moaA - molybdenum cofactor biosynthesis protein A (RefSeq), at 101,556 to 102,569
moaA
Shew_0088: Shew_0088 - molybdenum cofactor biosynthesis protein B (RefSeq), at 102,681 to 103,211
_0088
Shew_0089: moaC - molybdenum cofactor biosynthesis protein C (RefSeq), at 103,204 to 103,680
moaC
Shew_0090: Shew_0090 - molybdopterin converting factor, subunit 1 (RefSeq), at 103,730 to 103,981
_0090
Shew_0091: Shew_0091 - molybdopterin biosynthesis MoaE (RefSeq), at 103,983 to 104,450
_0091
Position (kb)
102
103
104 Strain fitness (log2 ratio)
-2
-1
0
1 at 101.755 kb on + strand, within moaA at 101.864 kb on - strand, within moaA at 101.956 kb on + strand, within moaA at 101.956 kb on + strand, within moaA at 101.956 kb on + strand, within moaA at 101.964 kb on - strand, within moaA at 101.964 kb on - strand, within moaA at 102.046 kb on - strand, within moaA at 102.118 kb on + strand, within moaA at 102.126 kb on - strand, within moaA at 102.445 kb on + strand, within moaA at 102.445 kb on + strand, within moaA at 102.445 kb on + strand, within moaA at 102.453 kb on - strand, within moaA at 102.453 kb on - strand, within moaA at 102.453 kb on - strand, within moaA at 102.453 kb on - strand, within moaA at 102.476 kb on + strand at 102.488 kb on + strand at 102.609 kb on + strand at 102.658 kb on + strand at 102.685 kb on - strand at 102.726 kb on + strand at 102.737 kb on + strand, within Shew_0088 at 102.753 kb on - strand, within Shew_0088 at 103.015 kb on + strand, within Shew_0088 at 103.023 kb on - strand, within Shew_0088 at 103.038 kb on + strand, within Shew_0088 at 103.143 kb on + strand, within Shew_0088 at 103.172 kb on - strand at 103.601 kb on - strand, within moaC at 104.069 kb on - strand, within Shew_0091 at 104.211 kb on + strand, within Shew_0091
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 101,755 + moaA Shew_0087 0.20 -1.1 101,864 - moaA Shew_0087 0.30 -0.2 101,956 + moaA Shew_0087 0.39 -0.3 101,956 + moaA Shew_0087 0.39 +0.0 101,956 + moaA Shew_0087 0.39 -0.8 101,964 - moaA Shew_0087 0.40 -2.1 101,964 - moaA Shew_0087 0.40 -0.4 102,046 - moaA Shew_0087 0.48 -1.6 102,118 + moaA Shew_0087 0.55 -0.2 102,126 - moaA Shew_0087 0.56 -0.4 102,445 + moaA Shew_0087 0.88 +0.8 102,445 + moaA Shew_0087 0.88 -0.0 102,445 + moaA Shew_0087 0.88 -0.2 102,453 - moaA Shew_0087 0.88 -0.6 102,453 - moaA Shew_0087 0.88 -0.2 102,453 - moaA Shew_0087 0.88 -0.1 102,453 - moaA Shew_0087 0.88 +0.0 102,476 + -0.9 102,488 + +0.0 102,609 + +0.5 102,658 + -0.5 102,685 - -0.3 102,726 + +0.0 102,737 + Shew_0088 0.11 -0.8 102,753 - Shew_0088 0.14 -1.4 103,015 + Shew_0088 0.63 +0.0 103,023 - Shew_0088 0.64 -1.7 103,038 + Shew_0088 0.67 +0.4 103,143 + Shew_0088 0.87 +0.1 103,172 - -1.0 103,601 - moaC Shew_0089 0.83 -0.7 104,069 - Shew_0091 0.18 -0.6 104,211 + Shew_0091 0.49 +0.1
Or see this region's nucleotide sequence