Experiment: BHIS with Benzethonium chloride 0.003 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2581 and BT2582 are separated by 104 nucleotides BT2582 and BT2583 are separated by 11 nucleotides BT2583 and BT2584 overlap by 8 nucleotides BT2584 and BT2585 are separated by 11 nucleotides
BT2581: BT2581 - hypothetical protein (NCBI ptt file), at 3,225,213 to 3,225,716
BT2581
BT2582: BT2582 - conserved hypothetical protein (NCBI ptt file), at 3,225,821 to 3,226,372
BT2582
BT2583: BT2583 - hypothetical protein (NCBI ptt file), at 3,226,384 to 3,227,310
BT2583
BT2584: BT2584 - hypothetical protein (NCBI ptt file), at 3,227,303 to 3,228,127
BT2584
BT2585: BT2585 - hypothetical protein (NCBI ptt file), at 3,228,139 to 3,228,432
BT2585
Position (kb)
3226
3227
3228 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3225.395 kb on + strand, within BT2581 at 3225.395 kb on + strand, within BT2581 at 3225.396 kb on - strand, within BT2581 at 3225.396 kb on - strand, within BT2581 at 3225.396 kb on - strand, within BT2581 at 3225.399 kb on + strand, within BT2581 at 3225.399 kb on + strand, within BT2581 at 3225.400 kb on - strand, within BT2581 at 3225.400 kb on - strand, within BT2581 at 3225.400 kb on - strand, within BT2581 at 3225.401 kb on + strand, within BT2581 at 3225.402 kb on - strand, within BT2581 at 3225.405 kb on + strand, within BT2581 at 3225.405 kb on + strand, within BT2581 at 3225.405 kb on + strand, within BT2581 at 3225.405 kb on + strand, within BT2581 at 3225.406 kb on - strand, within BT2581 at 3225.406 kb on - strand, within BT2581 at 3225.408 kb on - strand at 3225.413 kb on + strand, within BT2581 at 3225.418 kb on - strand, within BT2581 at 3225.535 kb on - strand, within BT2581 at 3225.535 kb on - strand, within BT2581 at 3225.970 kb on - strand, within BT2582 at 3226.026 kb on + strand, within BT2582 at 3226.058 kb on - strand, within BT2582 at 3226.058 kb on - strand, within BT2582 at 3226.058 kb on - strand, within BT2582 at 3226.177 kb on - strand, within BT2582 at 3226.370 kb on + strand at 3226.463 kb on + strand at 3226.474 kb on + strand at 3226.474 kb on + strand at 3226.477 kb on + strand, within BT2583 at 3226.477 kb on + strand, within BT2583 at 3226.550 kb on + strand, within BT2583 at 3226.619 kb on - strand, within BT2583 at 3226.621 kb on + strand, within BT2583 at 3226.624 kb on - strand, within BT2583 at 3226.624 kb on - strand, within BT2583 at 3226.630 kb on - strand, within BT2583 at 3226.702 kb on + strand, within BT2583 at 3226.703 kb on - strand, within BT2583 at 3226.703 kb on - strand, within BT2583 at 3226.703 kb on - strand, within BT2583 at 3226.703 kb on - strand, within BT2583 at 3226.703 kb on - strand, within BT2583 at 3226.851 kb on + strand, within BT2583 at 3226.852 kb on - strand, within BT2583 at 3226.994 kb on + strand, within BT2583 at 3226.995 kb on - strand, within BT2583 at 3227.027 kb on - strand, within BT2583 at 3227.027 kb on - strand, within BT2583 at 3227.093 kb on - strand, within BT2583 at 3227.100 kb on - strand, within BT2583 at 3227.102 kb on + strand, within BT2583 at 3227.201 kb on - strand, within BT2583 at 3227.226 kb on + strand at 3227.227 kb on - strand at 3227.227 kb on - strand at 3227.312 kb on - strand at 3227.331 kb on - strand at 3227.331 kb on - strand at 3227.355 kb on - strand at 3227.366 kb on + strand at 3227.437 kb on - strand, within BT2584 at 3227.439 kb on - strand, within BT2584 at 3227.459 kb on + strand, within BT2584 at 3227.494 kb on - strand, within BT2584 at 3227.696 kb on - strand, within BT2584 at 3227.706 kb on - strand, within BT2584 at 3227.754 kb on + strand, within BT2584 at 3227.783 kb on + strand, within BT2584 at 3227.783 kb on + strand, within BT2584 at 3227.783 kb on + strand, within BT2584 at 3227.784 kb on - strand, within BT2584 at 3227.992 kb on - strand, within BT2584 at 3227.997 kb on - strand, within BT2584 at 3228.083 kb on - strand at 3228.125 kb on + strand at 3228.126 kb on - strand at 3228.166 kb on + strand at 3228.166 kb on + strand at 3228.167 kb on - strand at 3228.167 kb on - strand at 3228.184 kb on + strand, within BT2585 at 3228.185 kb on - strand, within BT2585 at 3228.213 kb on - strand, within BT2585 at 3228.213 kb on - strand, within BT2585 at 3228.213 kb on - strand, within BT2585
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Benzethonium chloride 0.003 mM remove 3,225,395 + BT2581 0.36 +1.0 3,225,395 + BT2581 0.36 -3.2 3,225,396 - BT2581 0.36 -0.6 3,225,396 - BT2581 0.36 +1.0 3,225,396 - BT2581 0.36 -0.2 3,225,399 + BT2581 0.37 -1.0 3,225,399 + BT2581 0.37 -0.4 3,225,400 - BT2581 0.37 -0.3 3,225,400 - BT2581 0.37 -1.1 3,225,400 - BT2581 0.37 -1.0 3,225,401 + BT2581 0.37 -1.1 3,225,402 - BT2581 0.38 -0.9 3,225,405 + BT2581 0.38 -2.9 3,225,405 + BT2581 0.38 +0.0 3,225,405 + BT2581 0.38 -1.2 3,225,405 + BT2581 0.38 -1.8 3,225,406 - BT2581 0.38 -0.8 3,225,406 - BT2581 0.38 -0.7 3,225,408 - +0.0 3,225,413 + BT2581 0.40 -0.7 3,225,418 - BT2581 0.41 -0.6 3,225,535 - BT2581 0.64 +1.0 3,225,535 - BT2581 0.64 -0.7 3,225,970 - BT2582 0.27 -2.0 3,226,026 + BT2582 0.37 -0.7 3,226,058 - BT2582 0.43 -0.2 3,226,058 - BT2582 0.43 +0.4 3,226,058 - BT2582 0.43 +0.0 3,226,177 - BT2582 0.64 +1.0 3,226,370 + -0.8 3,226,463 + -0.6 3,226,474 + -0.8 3,226,474 + +1.0 3,226,477 + BT2583 0.10 +0.5 3,226,477 + BT2583 0.10 -1.0 3,226,550 + BT2583 0.18 -1.3 3,226,619 - BT2583 0.25 +0.1 3,226,621 + BT2583 0.26 +1.0 3,226,624 - BT2583 0.26 -0.5 3,226,624 - BT2583 0.26 -1.7 3,226,630 - BT2583 0.27 -1.0 3,226,702 + BT2583 0.34 +0.4 3,226,703 - BT2583 0.34 -2.0 3,226,703 - BT2583 0.34 +0.5 3,226,703 - BT2583 0.34 +0.3 3,226,703 - BT2583 0.34 +0.2 3,226,703 - BT2583 0.34 +0.0 3,226,851 + BT2583 0.50 +0.3 3,226,852 - BT2583 0.50 -0.5 3,226,994 + BT2583 0.66 -0.7 3,226,995 - BT2583 0.66 -0.7 3,227,027 - BT2583 0.69 -0.5 3,227,027 - BT2583 0.69 -0.1 3,227,093 - BT2583 0.76 +1.0 3,227,100 - BT2583 0.77 -0.5 3,227,102 + BT2583 0.77 -0.1 3,227,201 - BT2583 0.88 +1.0 3,227,226 + +0.0 3,227,227 - -0.4 3,227,227 - -1.0 3,227,312 - -1.5 3,227,331 - -0.6 3,227,331 - -1.3 3,227,355 - -1.6 3,227,366 + +1.0 3,227,437 - BT2584 0.16 -1.7 3,227,439 - BT2584 0.16 +0.0 3,227,459 + BT2584 0.19 +1.0 3,227,494 - BT2584 0.23 -0.6 3,227,696 - BT2584 0.48 -0.6 3,227,706 - BT2584 0.49 +1.0 3,227,754 + BT2584 0.55 -1.8 3,227,783 + BT2584 0.58 +0.0 3,227,783 + BT2584 0.58 +1.0 3,227,783 + BT2584 0.58 +1.6 3,227,784 - BT2584 0.58 -0.3 3,227,992 - BT2584 0.84 -0.7 3,227,997 - BT2584 0.84 -0.7 3,228,083 - -1.0 3,228,125 + -0.8 3,228,126 - -1.5 3,228,166 + +0.0 3,228,166 + +0.0 3,228,167 - -1.4 3,228,167 - +0.0 3,228,184 + BT2585 0.15 -0.2 3,228,185 - BT2585 0.16 +0.0 3,228,213 - BT2585 0.25 -1.7 3,228,213 - BT2585 0.25 +0.3 3,228,213 - BT2585 0.25 -0.7
Or see this region's nucleotide sequence