Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4716 and Pf6N2E2_4717 are separated by 308 nucleotides Pf6N2E2_4717 and Pf6N2E2_4718 are separated by 21 nucleotides
Pf6N2E2_4716 - Membrane protein, putative, at 5,452,907 to 5,453,611
_4716
Pf6N2E2_4717 - Serine hydroxymethyltransferase (EC 2.1.2.1), at 5,453,920 to 5,455,173
_4717
Pf6N2E2_4718 - Sarcosine oxidase beta subunit (EC 1.5.3.1), at 5,455,195 to 5,456,445
_4718
Position (kb)
5453
5454
5455
5456 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5452.983 kb on - strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.119 kb on - strand, within Pf6N2E2_4716 at 5453.119 kb on - strand, within Pf6N2E2_4716 at 5453.145 kb on - strand, within Pf6N2E2_4716 at 5453.272 kb on - strand, within Pf6N2E2_4716 at 5453.272 kb on - strand, within Pf6N2E2_4716 at 5453.364 kb on - strand, within Pf6N2E2_4716 at 5453.364 kb on - strand, within Pf6N2E2_4716 at 5453.420 kb on + strand, within Pf6N2E2_4716 at 5453.420 kb on + strand, within Pf6N2E2_4716 at 5453.420 kb on + strand, within Pf6N2E2_4716 at 5453.421 kb on - strand, within Pf6N2E2_4716 at 5453.552 kb on + strand at 5453.673 kb on - strand at 5453.784 kb on + strand at 5453.837 kb on + strand at 5453.838 kb on - strand at 5453.838 kb on - strand at 5453.838 kb on - strand at 5453.951 kb on - strand at 5454.032 kb on - strand at 5454.082 kb on + strand, within Pf6N2E2_4717 at 5454.112 kb on + strand, within Pf6N2E2_4717 at 5454.112 kb on + strand, within Pf6N2E2_4717 at 5454.113 kb on - strand, within Pf6N2E2_4717 at 5454.233 kb on + strand, within Pf6N2E2_4717 at 5454.233 kb on + strand, within Pf6N2E2_4717 at 5454.234 kb on - strand, within Pf6N2E2_4717 at 5454.234 kb on - strand, within Pf6N2E2_4717 at 5454.236 kb on - strand, within Pf6N2E2_4717 at 5454.337 kb on + strand, within Pf6N2E2_4717 at 5454.337 kb on + strand, within Pf6N2E2_4717 at 5454.338 kb on - strand, within Pf6N2E2_4717 at 5454.352 kb on + strand, within Pf6N2E2_4717 at 5454.385 kb on + strand, within Pf6N2E2_4717 at 5454.452 kb on - strand, within Pf6N2E2_4717 at 5454.845 kb on + strand, within Pf6N2E2_4717 at 5454.845 kb on + strand, within Pf6N2E2_4717 at 5454.845 kb on + strand, within Pf6N2E2_4717 at 5454.845 kb on + strand, within Pf6N2E2_4717 at 5454.846 kb on - strand, within Pf6N2E2_4717 at 5454.846 kb on - strand, within Pf6N2E2_4717 at 5454.846 kb on - strand, within Pf6N2E2_4717 at 5454.846 kb on - strand, within Pf6N2E2_4717 at 5454.846 kb on - strand, within Pf6N2E2_4717 at 5455.136 kb on + strand at 5455.187 kb on + strand at 5455.187 kb on + strand at 5455.188 kb on - strand at 5455.194 kb on + strand at 5455.194 kb on + strand at 5455.194 kb on + strand at 5455.194 kb on + strand at 5455.195 kb on - strand at 5455.195 kb on - strand at 5455.195 kb on - strand at 5455.204 kb on + strand at 5455.269 kb on + strand at 5455.291 kb on + strand at 5455.339 kb on + strand, within Pf6N2E2_4718 at 5455.339 kb on + strand, within Pf6N2E2_4718 at 5455.339 kb on + strand, within Pf6N2E2_4718 at 5455.340 kb on - strand, within Pf6N2E2_4718 at 5455.444 kb on + strand, within Pf6N2E2_4718 at 5455.445 kb on - strand, within Pf6N2E2_4718 at 5455.445 kb on - strand, within Pf6N2E2_4718 at 5455.445 kb on - strand, within Pf6N2E2_4718 at 5455.471 kb on + strand, within Pf6N2E2_4718 at 5455.472 kb on - strand, within Pf6N2E2_4718 at 5455.520 kb on - strand, within Pf6N2E2_4718 at 5455.549 kb on + strand, within Pf6N2E2_4718 at 5455.622 kb on + strand, within Pf6N2E2_4718 at 5455.623 kb on - strand, within Pf6N2E2_4718 at 5456.041 kb on + strand, within Pf6N2E2_4718 at 5456.095 kb on + strand, within Pf6N2E2_4718
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 5,452,983 - Pf6N2E2_4716 0.11 -0.3 5,453,009 + Pf6N2E2_4716 0.14 -0.1 5,453,009 + Pf6N2E2_4716 0.14 +0.1 5,453,009 + Pf6N2E2_4716 0.14 +0.0 5,453,009 + Pf6N2E2_4716 0.14 +0.5 5,453,009 + Pf6N2E2_4716 0.14 +0.1 5,453,010 - Pf6N2E2_4716 0.15 -1.0 5,453,010 - Pf6N2E2_4716 0.15 +0.4 5,453,010 - Pf6N2E2_4716 0.15 +1.2 5,453,010 - Pf6N2E2_4716 0.15 -1.0 5,453,010 - Pf6N2E2_4716 0.15 +0.6 5,453,010 - Pf6N2E2_4716 0.15 -1.9 5,453,010 - Pf6N2E2_4716 0.15 +0.2 5,453,010 - Pf6N2E2_4716 0.15 -0.8 5,453,119 - Pf6N2E2_4716 0.30 +0.4 5,453,119 - Pf6N2E2_4716 0.30 +0.1 5,453,145 - Pf6N2E2_4716 0.34 -0.5 5,453,272 - Pf6N2E2_4716 0.52 -0.7 5,453,272 - Pf6N2E2_4716 0.52 -0.1 5,453,364 - Pf6N2E2_4716 0.65 -0.8 5,453,364 - Pf6N2E2_4716 0.65 -3.3 5,453,420 + Pf6N2E2_4716 0.73 -1.2 5,453,420 + Pf6N2E2_4716 0.73 +0.5 5,453,420 + Pf6N2E2_4716 0.73 +0.2 5,453,421 - Pf6N2E2_4716 0.73 -0.0 5,453,552 + -0.6 5,453,673 - +0.7 5,453,784 + +1.0 5,453,837 + +0.7 5,453,838 - +0.5 5,453,838 - +1.2 5,453,838 - -0.1 5,453,951 - +0.3 5,454,032 - +0.3 5,454,082 + Pf6N2E2_4717 0.13 -2.1 5,454,112 + Pf6N2E2_4717 0.15 +1.4 5,454,112 + Pf6N2E2_4717 0.15 +0.6 5,454,113 - Pf6N2E2_4717 0.15 +0.9 5,454,233 + Pf6N2E2_4717 0.25 +0.1 5,454,233 + Pf6N2E2_4717 0.25 -0.1 5,454,234 - Pf6N2E2_4717 0.25 +1.1 5,454,234 - Pf6N2E2_4717 0.25 -0.1 5,454,236 - Pf6N2E2_4717 0.25 -0.4 5,454,337 + Pf6N2E2_4717 0.33 -0.2 5,454,337 + Pf6N2E2_4717 0.33 -0.1 5,454,338 - Pf6N2E2_4717 0.33 +0.8 5,454,352 + Pf6N2E2_4717 0.34 +0.6 5,454,385 + Pf6N2E2_4717 0.37 +1.1 5,454,452 - Pf6N2E2_4717 0.42 -0.5 5,454,845 + Pf6N2E2_4717 0.74 +0.4 5,454,845 + Pf6N2E2_4717 0.74 +0.5 5,454,845 + Pf6N2E2_4717 0.74 +0.2 5,454,845 + Pf6N2E2_4717 0.74 -1.6 5,454,846 - Pf6N2E2_4717 0.74 +1.7 5,454,846 - Pf6N2E2_4717 0.74 -0.1 5,454,846 - Pf6N2E2_4717 0.74 -0.0 5,454,846 - Pf6N2E2_4717 0.74 -1.2 5,454,846 - Pf6N2E2_4717 0.74 +1.2 5,455,136 + +0.9 5,455,187 + -1.1 5,455,187 + -2.5 5,455,188 - +0.6 5,455,194 + +0.7 5,455,194 + +0.0 5,455,194 + +1.1 5,455,194 + +0.3 5,455,195 - -0.5 5,455,195 - -1.2 5,455,195 - +0.9 5,455,204 + +0.8 5,455,269 + -0.0 5,455,291 + -0.7 5,455,339 + Pf6N2E2_4718 0.12 -0.5 5,455,339 + Pf6N2E2_4718 0.12 -1.0 5,455,339 + Pf6N2E2_4718 0.12 -1.9 5,455,340 - Pf6N2E2_4718 0.12 +0.9 5,455,444 + Pf6N2E2_4718 0.20 +0.6 5,455,445 - Pf6N2E2_4718 0.20 +0.7 5,455,445 - Pf6N2E2_4718 0.20 +0.1 5,455,445 - Pf6N2E2_4718 0.20 +0.2 5,455,471 + Pf6N2E2_4718 0.22 +0.9 5,455,472 - Pf6N2E2_4718 0.22 +1.0 5,455,520 - Pf6N2E2_4718 0.26 -0.6 5,455,549 + Pf6N2E2_4718 0.28 -2.7 5,455,622 + Pf6N2E2_4718 0.34 -0.5 5,455,623 - Pf6N2E2_4718 0.34 -1.2 5,456,041 + Pf6N2E2_4718 0.68 +0.6 5,456,095 + Pf6N2E2_4718 0.72 +0.2
Or see this region's nucleotide sequence