Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3908 and Pf6N2E2_3909 are separated by 250 nucleotides Pf6N2E2_3909 and Pf6N2E2_3910 are separated by 21 nucleotides Pf6N2E2_3910 and Pf6N2E2_3911 overlap by 4 nucleotides
Pf6N2E2_3908 - Glutamate--cysteine ligase (EC 6.3.2.2), at 4,542,782 to 4,544,380
_3908
Pf6N2E2_3909 - Taurine-binding periplasmic protein TauA, at 4,544,631 to 4,545,608
_3909
Pf6N2E2_3910 - Taurine transport ATP-binding protein TauB, at 4,545,630 to 4,546,424
_3910
Pf6N2E2_3911 - Taurine transport system permease protein TauC, at 4,546,421 to 4,547,260
_3911
Position (kb)
4544
4545
4546 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4543.666 kb on + strand, within Pf6N2E2_3908 at 4543.694 kb on + strand, within Pf6N2E2_3908 at 4543.695 kb on - strand, within Pf6N2E2_3908 at 4543.695 kb on - strand, within Pf6N2E2_3908 at 4543.697 kb on + strand, within Pf6N2E2_3908 at 4543.697 kb on + strand, within Pf6N2E2_3908 at 4543.727 kb on + strand, within Pf6N2E2_3908 at 4543.776 kb on - strand, within Pf6N2E2_3908 at 4543.865 kb on + strand, within Pf6N2E2_3908 at 4543.866 kb on - strand, within Pf6N2E2_3908 at 4544.349 kb on - strand at 4544.374 kb on + strand at 4544.374 kb on + strand at 4544.374 kb on + strand at 4544.375 kb on - strand at 4544.375 kb on - strand at 4544.375 kb on - strand at 4544.508 kb on - strand at 4544.770 kb on - strand, within Pf6N2E2_3909 at 4545.045 kb on + strand, within Pf6N2E2_3909 at 4545.046 kb on - strand, within Pf6N2E2_3909 at 4545.046 kb on - strand, within Pf6N2E2_3909 at 4545.046 kb on - strand, within Pf6N2E2_3909 at 4545.046 kb on - strand, within Pf6N2E2_3909 at 4545.100 kb on + strand, within Pf6N2E2_3909 at 4545.162 kb on + strand, within Pf6N2E2_3909 at 4545.162 kb on + strand, within Pf6N2E2_3909 at 4545.162 kb on + strand, within Pf6N2E2_3909 at 4545.162 kb on + strand, within Pf6N2E2_3909 at 4545.162 kb on + strand, within Pf6N2E2_3909 at 4545.163 kb on - strand, within Pf6N2E2_3909 at 4545.163 kb on - strand, within Pf6N2E2_3909 at 4545.163 kb on - strand, within Pf6N2E2_3909 at 4545.163 kb on - strand, within Pf6N2E2_3909 at 4545.163 kb on - strand, within Pf6N2E2_3909 at 4545.163 kb on - strand, within Pf6N2E2_3909 at 4545.336 kb on + strand, within Pf6N2E2_3909 at 4545.336 kb on + strand, within Pf6N2E2_3909 at 4545.570 kb on + strand at 4545.571 kb on - strand at 4545.571 kb on - strand at 4545.580 kb on - strand at 4545.594 kb on + strand at 4545.594 kb on + strand at 4545.594 kb on + strand at 4545.594 kb on + strand at 4545.594 kb on + strand at 4545.594 kb on + strand at 4545.594 kb on + strand at 4545.595 kb on - strand at 4545.595 kb on - strand at 4545.595 kb on - strand at 4545.595 kb on - strand at 4545.595 kb on - strand at 4545.595 kb on - strand at 4545.595 kb on - strand at 4545.595 kb on - strand at 4545.667 kb on - strand at 4545.667 kb on - strand at 4546.016 kb on + strand, within Pf6N2E2_3910 at 4546.017 kb on - strand, within Pf6N2E2_3910 at 4546.150 kb on + strand, within Pf6N2E2_3910 at 4546.150 kb on + strand, within Pf6N2E2_3910 at 4546.150 kb on + strand, within Pf6N2E2_3910 at 4546.150 kb on + strand, within Pf6N2E2_3910 at 4546.150 kb on + strand, within Pf6N2E2_3910 at 4546.151 kb on - strand, within Pf6N2E2_3910 at 4546.151 kb on - strand, within Pf6N2E2_3910 at 4546.279 kb on + strand, within Pf6N2E2_3910 at 4546.280 kb on - strand, within Pf6N2E2_3910 at 4546.280 kb on - strand, within Pf6N2E2_3910 at 4546.431 kb on - strand at 4546.485 kb on + strand at 4546.486 kb on - strand at 4546.486 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 4,543,666 + Pf6N2E2_3908 0.55 -1.6 4,543,694 + Pf6N2E2_3908 0.57 -2.2 4,543,695 - Pf6N2E2_3908 0.57 -1.3 4,543,695 - Pf6N2E2_3908 0.57 -0.2 4,543,697 + Pf6N2E2_3908 0.57 -0.1 4,543,697 + Pf6N2E2_3908 0.57 -1.8 4,543,727 + Pf6N2E2_3908 0.59 +1.7 4,543,776 - Pf6N2E2_3908 0.62 +0.1 4,543,865 + Pf6N2E2_3908 0.68 -0.6 4,543,866 - Pf6N2E2_3908 0.68 +1.1 4,544,349 - +0.8 4,544,374 + -0.2 4,544,374 + +0.3 4,544,374 + +1.7 4,544,375 - -0.2 4,544,375 - -0.3 4,544,375 - -0.8 4,544,508 - -0.8 4,544,770 - Pf6N2E2_3909 0.14 +0.7 4,545,045 + Pf6N2E2_3909 0.42 +0.0 4,545,046 - Pf6N2E2_3909 0.42 +0.4 4,545,046 - Pf6N2E2_3909 0.42 +0.7 4,545,046 - Pf6N2E2_3909 0.42 +1.2 4,545,046 - Pf6N2E2_3909 0.42 +0.2 4,545,100 + Pf6N2E2_3909 0.48 -0.2 4,545,162 + Pf6N2E2_3909 0.54 -0.2 4,545,162 + Pf6N2E2_3909 0.54 +0.3 4,545,162 + Pf6N2E2_3909 0.54 +0.8 4,545,162 + Pf6N2E2_3909 0.54 -1.2 4,545,162 + Pf6N2E2_3909 0.54 +1.3 4,545,163 - Pf6N2E2_3909 0.54 +0.0 4,545,163 - Pf6N2E2_3909 0.54 +1.1 4,545,163 - Pf6N2E2_3909 0.54 +1.2 4,545,163 - Pf6N2E2_3909 0.54 -0.2 4,545,163 - Pf6N2E2_3909 0.54 -0.6 4,545,163 - Pf6N2E2_3909 0.54 +1.2 4,545,336 + Pf6N2E2_3909 0.72 +0.4 4,545,336 + Pf6N2E2_3909 0.72 +0.4 4,545,570 + -0.1 4,545,571 - +0.2 4,545,571 - -0.9 4,545,580 - -0.8 4,545,594 + -0.3 4,545,594 + -0.4 4,545,594 + -0.4 4,545,594 + +0.0 4,545,594 + -0.4 4,545,594 + -1.3 4,545,594 + -0.4 4,545,595 - -0.0 4,545,595 - +1.1 4,545,595 - +0.2 4,545,595 - +0.2 4,545,595 - -0.5 4,545,595 - -0.3 4,545,595 - +0.3 4,545,595 - +0.1 4,545,667 - +0.6 4,545,667 - +1.0 4,546,016 + Pf6N2E2_3910 0.49 -0.3 4,546,017 - Pf6N2E2_3910 0.49 +0.8 4,546,150 + Pf6N2E2_3910 0.65 -2.0 4,546,150 + Pf6N2E2_3910 0.65 -0.6 4,546,150 + Pf6N2E2_3910 0.65 -0.8 4,546,150 + Pf6N2E2_3910 0.65 +1.5 4,546,150 + Pf6N2E2_3910 0.65 -0.0 4,546,151 - Pf6N2E2_3910 0.66 -0.5 4,546,151 - Pf6N2E2_3910 0.66 -0.2 4,546,279 + Pf6N2E2_3910 0.82 -2.2 4,546,280 - Pf6N2E2_3910 0.82 +2.6 4,546,280 - Pf6N2E2_3910 0.82 +0.8 4,546,431 - -0.5 4,546,485 + -1.0 4,546,486 - -0.8 4,546,486 - +0.3
Or see this region's nucleotide sequence