Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1558 and Pf6N2E2_1559 overlap by 20 nucleotides Pf6N2E2_1559 and Pf6N2E2_1560 are separated by 174 nucleotides
Pf6N2E2_1558 - Sensor histidine kinase/response regulator, at 1,818,743 to 1,820,437
_1558
Pf6N2E2_1559 - Methyl-accepting chemotaxis protein, at 1,820,418 to 1,821,683
_1559
Pf6N2E2_1560 - Glycine cleavage system transcriptional activator GcvA, at 1,821,858 to 1,822,763
_1560
Position (kb)
1820
1821
1822 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1819.486 kb on - strand, within Pf6N2E2_1558 at 1819.508 kb on + strand, within Pf6N2E2_1558 at 1819.508 kb on + strand, within Pf6N2E2_1558 at 1819.508 kb on + strand, within Pf6N2E2_1558 at 1819.508 kb on + strand, within Pf6N2E2_1558 at 1819.508 kb on + strand, within Pf6N2E2_1558 at 1819.509 kb on - strand at 1819.509 kb on - strand, within Pf6N2E2_1558 at 1819.551 kb on + strand, within Pf6N2E2_1558 at 1819.552 kb on - strand, within Pf6N2E2_1558 at 1819.675 kb on + strand, within Pf6N2E2_1558 at 1819.676 kb on - strand, within Pf6N2E2_1558 at 1819.712 kb on + strand, within Pf6N2E2_1558 at 1819.712 kb on + strand, within Pf6N2E2_1558 at 1819.712 kb on + strand, within Pf6N2E2_1558 at 1819.713 kb on - strand, within Pf6N2E2_1558 at 1819.713 kb on - strand, within Pf6N2E2_1558 at 1819.713 kb on - strand at 1819.713 kb on - strand, within Pf6N2E2_1558 at 1819.713 kb on - strand, within Pf6N2E2_1558 at 1819.778 kb on + strand, within Pf6N2E2_1558 at 1819.778 kb on + strand, within Pf6N2E2_1558 at 1819.778 kb on + strand, within Pf6N2E2_1558 at 1819.779 kb on - strand, within Pf6N2E2_1558 at 1819.929 kb on + strand, within Pf6N2E2_1558 at 1819.929 kb on + strand, within Pf6N2E2_1558 at 1819.929 kb on + strand, within Pf6N2E2_1558 at 1819.930 kb on - strand, within Pf6N2E2_1558 at 1819.930 kb on - strand, within Pf6N2E2_1558 at 1819.930 kb on - strand, within Pf6N2E2_1558 at 1819.930 kb on - strand, within Pf6N2E2_1558 at 1819.930 kb on - strand, within Pf6N2E2_1558 at 1819.930 kb on - strand, within Pf6N2E2_1558 at 1819.930 kb on - strand, within Pf6N2E2_1558 at 1819.930 kb on - strand, within Pf6N2E2_1558 at 1820.047 kb on - strand, within Pf6N2E2_1558 at 1820.079 kb on + strand, within Pf6N2E2_1558 at 1820.079 kb on + strand, within Pf6N2E2_1558 at 1820.079 kb on + strand, within Pf6N2E2_1558 at 1820.229 kb on + strand, within Pf6N2E2_1558 at 1820.954 kb on + strand, within Pf6N2E2_1559 at 1820.955 kb on - strand, within Pf6N2E2_1559 at 1820.955 kb on - strand, within Pf6N2E2_1559 at 1821.062 kb on - strand, within Pf6N2E2_1559 at 1821.062 kb on - strand, within Pf6N2E2_1559 at 1821.073 kb on + strand, within Pf6N2E2_1559 at 1821.172 kb on + strand, within Pf6N2E2_1559 at 1821.244 kb on + strand, within Pf6N2E2_1559 at 1821.244 kb on + strand, within Pf6N2E2_1559 at 1821.391 kb on + strand, within Pf6N2E2_1559 at 1821.391 kb on + strand, within Pf6N2E2_1559 at 1821.391 kb on + strand, within Pf6N2E2_1559 at 1821.392 kb on - strand, within Pf6N2E2_1559 at 1821.437 kb on + strand, within Pf6N2E2_1559 at 1821.439 kb on + strand, within Pf6N2E2_1559 at 1821.439 kb on + strand, within Pf6N2E2_1559 at 1821.439 kb on + strand, within Pf6N2E2_1559 at 1821.439 kb on + strand, within Pf6N2E2_1559 at 1821.439 kb on + strand, within Pf6N2E2_1559 at 1821.440 kb on - strand, within Pf6N2E2_1559 at 1821.703 kb on - strand at 1821.715 kb on + strand at 1821.716 kb on - strand at 1821.716 kb on - strand at 1821.716 kb on - strand at 1821.716 kb on - strand at 1821.716 kb on - strand at 1821.716 kb on - strand at 1821.716 kb on - strand at 1821.716 kb on - strand at 1821.716 kb on - strand at 1821.764 kb on + strand at 1821.764 kb on + strand at 1821.765 kb on - strand at 1821.778 kb on + strand at 1821.779 kb on - strand at 1821.813 kb on + strand at 1821.813 kb on + strand at 1821.813 kb on + strand at 1821.813 kb on + strand at 1821.813 kb on + strand at 1821.813 kb on + strand at 1821.814 kb on - strand at 1821.814 kb on - strand at 1821.814 kb on - strand at 1821.903 kb on - strand at 1821.903 kb on - strand at 1822.028 kb on - strand, within Pf6N2E2_1560 at 1822.373 kb on + strand, within Pf6N2E2_1560 at 1822.373 kb on + strand, within Pf6N2E2_1560 at 1822.558 kb on + strand, within Pf6N2E2_1560 at 1822.558 kb on + strand, within Pf6N2E2_1560
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 1,819,486 - Pf6N2E2_1558 0.44 +0.1 1,819,508 + Pf6N2E2_1558 0.45 +0.5 1,819,508 + Pf6N2E2_1558 0.45 +0.1 1,819,508 + Pf6N2E2_1558 0.45 -0.3 1,819,508 + Pf6N2E2_1558 0.45 +0.7 1,819,508 + Pf6N2E2_1558 0.45 -0.3 1,819,509 - +1.3 1,819,509 - Pf6N2E2_1558 0.45 +0.4 1,819,551 + Pf6N2E2_1558 0.48 +0.4 1,819,552 - Pf6N2E2_1558 0.48 +0.3 1,819,675 + Pf6N2E2_1558 0.55 +0.7 1,819,676 - Pf6N2E2_1558 0.55 +0.8 1,819,712 + Pf6N2E2_1558 0.57 +0.9 1,819,712 + Pf6N2E2_1558 0.57 -0.1 1,819,712 + Pf6N2E2_1558 0.57 +0.1 1,819,713 - Pf6N2E2_1558 0.57 -1.0 1,819,713 - Pf6N2E2_1558 0.57 +0.2 1,819,713 - -0.3 1,819,713 - Pf6N2E2_1558 0.57 -0.0 1,819,713 - Pf6N2E2_1558 0.57 +1.3 1,819,778 + Pf6N2E2_1558 0.61 +0.3 1,819,778 + Pf6N2E2_1558 0.61 -0.3 1,819,778 + Pf6N2E2_1558 0.61 -1.5 1,819,779 - Pf6N2E2_1558 0.61 +0.1 1,819,929 + Pf6N2E2_1558 0.70 +0.2 1,819,929 + Pf6N2E2_1558 0.70 -0.9 1,819,929 + Pf6N2E2_1558 0.70 +0.9 1,819,930 - Pf6N2E2_1558 0.70 -1.9 1,819,930 - Pf6N2E2_1558 0.70 -1.7 1,819,930 - Pf6N2E2_1558 0.70 +0.9 1,819,930 - Pf6N2E2_1558 0.70 -1.7 1,819,930 - Pf6N2E2_1558 0.70 +2.0 1,819,930 - Pf6N2E2_1558 0.70 -0.4 1,819,930 - Pf6N2E2_1558 0.70 +1.5 1,819,930 - Pf6N2E2_1558 0.70 +0.6 1,820,047 - Pf6N2E2_1558 0.77 -1.5 1,820,079 + Pf6N2E2_1558 0.79 -0.9 1,820,079 + Pf6N2E2_1558 0.79 -0.1 1,820,079 + Pf6N2E2_1558 0.79 +1.5 1,820,229 + Pf6N2E2_1558 0.88 +2.4 1,820,954 + Pf6N2E2_1559 0.42 +2.3 1,820,955 - Pf6N2E2_1559 0.42 +0.7 1,820,955 - Pf6N2E2_1559 0.42 +0.1 1,821,062 - Pf6N2E2_1559 0.51 +1.3 1,821,062 - Pf6N2E2_1559 0.51 -1.7 1,821,073 + Pf6N2E2_1559 0.52 -3.3 1,821,172 + Pf6N2E2_1559 0.60 -1.9 1,821,244 + Pf6N2E2_1559 0.65 -1.2 1,821,244 + Pf6N2E2_1559 0.65 -1.1 1,821,391 + Pf6N2E2_1559 0.77 -0.3 1,821,391 + Pf6N2E2_1559 0.77 -0.3 1,821,391 + Pf6N2E2_1559 0.77 +0.0 1,821,392 - Pf6N2E2_1559 0.77 -0.1 1,821,437 + Pf6N2E2_1559 0.80 +0.6 1,821,439 + Pf6N2E2_1559 0.81 -0.9 1,821,439 + Pf6N2E2_1559 0.81 +0.3 1,821,439 + Pf6N2E2_1559 0.81 -0.7 1,821,439 + Pf6N2E2_1559 0.81 -0.1 1,821,439 + Pf6N2E2_1559 0.81 +0.7 1,821,440 - Pf6N2E2_1559 0.81 +2.0 1,821,703 - +0.3 1,821,715 + -0.3 1,821,716 - +0.2 1,821,716 - -0.7 1,821,716 - -0.9 1,821,716 - -0.2 1,821,716 - -1.6 1,821,716 - -0.3 1,821,716 - -1.2 1,821,716 - +0.7 1,821,716 - -1.2 1,821,764 + +0.3 1,821,764 + +1.0 1,821,765 - +0.7 1,821,778 + +0.7 1,821,779 - -0.0 1,821,813 + -0.9 1,821,813 + -1.9 1,821,813 + +0.6 1,821,813 + +1.0 1,821,813 + +1.4 1,821,813 + +0.1 1,821,814 - +1.3 1,821,814 - +1.3 1,821,814 - +0.2 1,821,903 - +0.0 1,821,903 - -1.9 1,822,028 - Pf6N2E2_1560 0.19 -0.1 1,822,373 + Pf6N2E2_1560 0.57 -0.3 1,822,373 + Pf6N2E2_1560 0.57 -1.1 1,822,558 + Pf6N2E2_1560 0.77 -0.0 1,822,558 + Pf6N2E2_1560 0.77 +0.8
Or see this region's nucleotide sequence