Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1127 and Pf6N2E2_1128 overlap by 10 nucleotides Pf6N2E2_1128 and Pf6N2E2_1129 overlap by 4 nucleotides Pf6N2E2_1129 and Pf6N2E2_1130 are separated by 173 nucleotides Pf6N2E2_1130 and Pf6N2E2_1131 are separated by 279 nucleotides
Pf6N2E2_1127 - Sensory box histidine kinase/response regulator, at 1,322,314 to 1,324,632
_1127
Pf6N2E2_1128 - Two-component response regulator, at 1,324,623 to 1,325,291
_1128
Pf6N2E2_1129 - Sensor histidine kinase, at 1,325,288 to 1,326,472
_1129
Pf6N2E2_1130 - hypothetical protein, at 1,326,646 to 1,327,167
_1130
Pf6N2E2_1131 - RNA polymerase sigma-70 factor, ECF subfamily, at 1,327,447 to 1,327,866
_1131
Position (kb)
1325
1326
1327 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1324.362 kb on - strand, within Pf6N2E2_1127 at 1324.362 kb on - strand, within Pf6N2E2_1127 at 1324.362 kb on - strand, within Pf6N2E2_1127 at 1324.397 kb on + strand, within Pf6N2E2_1127 at 1324.405 kb on + strand at 1324.405 kb on + strand at 1324.405 kb on + strand at 1324.406 kb on - strand at 1324.435 kb on - strand at 1324.516 kb on - strand at 1324.517 kb on + strand at 1324.517 kb on + strand at 1324.517 kb on + strand at 1324.518 kb on - strand at 1324.518 kb on - strand at 1324.518 kb on - strand at 1324.518 kb on - strand at 1324.518 kb on - strand at 1324.726 kb on - strand, within Pf6N2E2_1128 at 1324.982 kb on - strand, within Pf6N2E2_1128 at 1325.231 kb on + strand at 1325.688 kb on + strand, within Pf6N2E2_1129 at 1325.688 kb on + strand, within Pf6N2E2_1129 at 1325.708 kb on + strand, within Pf6N2E2_1129 at 1325.890 kb on - strand, within Pf6N2E2_1129 at 1326.101 kb on + strand, within Pf6N2E2_1129 at 1326.101 kb on + strand, within Pf6N2E2_1129 at 1326.102 kb on - strand, within Pf6N2E2_1129 at 1326.102 kb on - strand, within Pf6N2E2_1129 at 1326.102 kb on - strand, within Pf6N2E2_1129 at 1326.102 kb on - strand, within Pf6N2E2_1129 at 1326.522 kb on + strand at 1326.523 kb on - strand at 1326.548 kb on - strand at 1326.548 kb on - strand at 1326.678 kb on - strand at 1326.724 kb on + strand, within Pf6N2E2_1130 at 1326.724 kb on + strand, within Pf6N2E2_1130 at 1326.724 kb on + strand, within Pf6N2E2_1130 at 1326.725 kb on - strand, within Pf6N2E2_1130 at 1326.725 kb on - strand, within Pf6N2E2_1130 at 1326.725 kb on - strand, within Pf6N2E2_1130 at 1326.725 kb on - strand, within Pf6N2E2_1130 at 1326.730 kb on + strand, within Pf6N2E2_1130 at 1326.894 kb on + strand, within Pf6N2E2_1130 at 1326.894 kb on + strand, within Pf6N2E2_1130 at 1326.894 kb on + strand, within Pf6N2E2_1130 at 1326.894 kb on + strand, within Pf6N2E2_1130 at 1326.895 kb on - strand, within Pf6N2E2_1130 at 1326.917 kb on - strand, within Pf6N2E2_1130 at 1327.012 kb on + strand, within Pf6N2E2_1130 at 1327.013 kb on - strand, within Pf6N2E2_1130 at 1327.013 kb on - strand, within Pf6N2E2_1130 at 1327.013 kb on - strand, within Pf6N2E2_1130 at 1327.014 kb on + strand, within Pf6N2E2_1130 at 1327.014 kb on + strand, within Pf6N2E2_1130 at 1327.078 kb on + strand, within Pf6N2E2_1130 at 1327.078 kb on + strand, within Pf6N2E2_1130 at 1327.078 kb on + strand, within Pf6N2E2_1130 at 1327.079 kb on - strand, within Pf6N2E2_1130 at 1327.238 kb on + strand at 1327.255 kb on + strand at 1327.269 kb on + strand at 1327.269 kb on + strand at 1327.282 kb on + strand at 1327.282 kb on + strand at 1327.283 kb on - strand at 1327.283 kb on - strand at 1327.313 kb on - strand at 1327.402 kb on + strand at 1327.402 kb on + strand at 1327.416 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 1,324,362 - Pf6N2E2_1127 0.88 +0.3 1,324,362 - Pf6N2E2_1127 0.88 -0.1 1,324,362 - Pf6N2E2_1127 0.88 +0.8 1,324,397 + Pf6N2E2_1127 0.90 +1.3 1,324,405 + +1.9 1,324,405 + +0.2 1,324,405 + -0.7 1,324,406 - -1.1 1,324,435 - -0.5 1,324,516 - -0.1 1,324,517 + +0.3 1,324,517 + +0.3 1,324,517 + +0.3 1,324,518 - -0.1 1,324,518 - -0.5 1,324,518 - -1.1 1,324,518 - -0.1 1,324,518 - +0.3 1,324,726 - Pf6N2E2_1128 0.15 +0.2 1,324,982 - Pf6N2E2_1128 0.54 +1.0 1,325,231 + +1.0 1,325,688 + Pf6N2E2_1129 0.34 +0.7 1,325,688 + Pf6N2E2_1129 0.34 +0.3 1,325,708 + Pf6N2E2_1129 0.35 -1.3 1,325,890 - Pf6N2E2_1129 0.51 +0.3 1,326,101 + Pf6N2E2_1129 0.69 -0.5 1,326,101 + Pf6N2E2_1129 0.69 -2.7 1,326,102 - Pf6N2E2_1129 0.69 -1.3 1,326,102 - Pf6N2E2_1129 0.69 -0.5 1,326,102 - Pf6N2E2_1129 0.69 -0.1 1,326,102 - Pf6N2E2_1129 0.69 -0.4 1,326,522 + +1.3 1,326,523 - +0.3 1,326,548 - -0.9 1,326,548 - +0.4 1,326,678 - +0.5 1,326,724 + Pf6N2E2_1130 0.15 -2.1 1,326,724 + Pf6N2E2_1130 0.15 +1.3 1,326,724 + Pf6N2E2_1130 0.15 +0.3 1,326,725 - Pf6N2E2_1130 0.15 -0.3 1,326,725 - Pf6N2E2_1130 0.15 +3.4 1,326,725 - Pf6N2E2_1130 0.15 -1.5 1,326,725 - Pf6N2E2_1130 0.15 -0.2 1,326,730 + Pf6N2E2_1130 0.16 -0.3 1,326,894 + Pf6N2E2_1130 0.48 -0.9 1,326,894 + Pf6N2E2_1130 0.48 +0.7 1,326,894 + Pf6N2E2_1130 0.48 -0.2 1,326,894 + Pf6N2E2_1130 0.48 +1.9 1,326,895 - Pf6N2E2_1130 0.48 -0.7 1,326,917 - Pf6N2E2_1130 0.52 -0.1 1,327,012 + Pf6N2E2_1130 0.70 -2.0 1,327,013 - Pf6N2E2_1130 0.70 -0.3 1,327,013 - Pf6N2E2_1130 0.70 -0.7 1,327,013 - Pf6N2E2_1130 0.70 +0.3 1,327,014 + Pf6N2E2_1130 0.70 +1.3 1,327,014 + Pf6N2E2_1130 0.70 -1.7 1,327,078 + Pf6N2E2_1130 0.83 +0.5 1,327,078 + Pf6N2E2_1130 0.83 -0.1 1,327,078 + Pf6N2E2_1130 0.83 -1.1 1,327,079 - Pf6N2E2_1130 0.83 -0.7 1,327,238 + -0.5 1,327,255 + +0.3 1,327,269 + +0.1 1,327,269 + +0.3 1,327,282 + +0.4 1,327,282 + -0.1 1,327,283 - +0.9 1,327,283 - -0.1 1,327,313 - +1.3 1,327,402 + +1.1 1,327,402 + +0.5 1,327,416 + +0.2
Or see this region's nucleotide sequence