Experiment: Minimal media pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_02995 and AO353_03000 are separated by 12 nucleotides AO353_03000 and AO353_03005 are separated by 63 nucleotides
AO353_02995: AO353_02995 - succinylglutamate desuccinylase, at 635,209 to 636,219
_02995
AO353_03000: AO353_03000 - topoisomerase II, at 636,232 to 636,519
_03000
AO353_03005: AO353_03005 - succinylarginine dihydrolase, at 636,583 to 637,929
_03005
Position (kb) 636
637 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 635.351 kb on + strand, within AO353_02995 at 635.352 kb on - strand, within AO353_02995 at 635.609 kb on + strand, within AO353_02995 at 635.609 kb on + strand, within AO353_02995 at 635.609 kb on + strand, within AO353_02995 at 635.610 kb on - strand, within AO353_02995 at 635.610 kb on - strand, within AO353_02995 at 635.610 kb on - strand, within AO353_02995 at 635.610 kb on - strand, within AO353_02995 at 635.610 kb on - strand, within AO353_02995 at 635.615 kb on + strand, within AO353_02995 at 635.616 kb on - strand, within AO353_02995 at 635.616 kb on - strand, within AO353_02995 at 635.726 kb on + strand, within AO353_02995 at 635.777 kb on + strand, within AO353_02995 at 635.778 kb on - strand, within AO353_02995 at 635.961 kb on + strand, within AO353_02995 at 635.961 kb on + strand, within AO353_02995 at 635.961 kb on + strand, within AO353_02995 at 635.961 kb on + strand, within AO353_02995 at 635.961 kb on + strand, within AO353_02995 at 635.961 kb on + strand, within AO353_02995 at 635.962 kb on - strand, within AO353_02995 at 635.962 kb on - strand, within AO353_02995 at 635.962 kb on - strand, within AO353_02995 at 636.017 kb on - strand, within AO353_02995 at 636.017 kb on - strand, within AO353_02995 at 636.219 kb on + strand at 636.219 kb on + strand at 636.219 kb on + strand at 636.219 kb on + strand at 636.219 kb on + strand at 636.219 kb on + strand at 636.219 kb on + strand at 636.220 kb on - strand at 636.220 kb on - strand at 636.313 kb on + strand, within AO353_03000 at 636.313 kb on + strand, within AO353_03000 at 636.314 kb on - strand, within AO353_03000 at 636.314 kb on - strand, within AO353_03000 at 636.322 kb on - strand, within AO353_03000 at 636.523 kb on + strand at 636.541 kb on + strand at 636.541 kb on + strand at 636.541 kb on + strand at 636.542 kb on - strand at 636.542 kb on - strand at 636.542 kb on - strand at 636.542 kb on - strand at 636.542 kb on - strand at 636.542 kb on - strand at 636.603 kb on - strand at 636.619 kb on - strand at 636.741 kb on - strand, within AO353_03005 at 636.741 kb on - strand, within AO353_03005 at 636.741 kb on - strand, within AO353_03005 at 636.741 kb on - strand, within AO353_03005 at 636.741 kb on - strand, within AO353_03005 at 636.741 kb on - strand, within AO353_03005 at 636.808 kb on + strand, within AO353_03005 at 636.911 kb on + strand, within AO353_03005 at 636.911 kb on + strand, within AO353_03005 at 636.911 kb on + strand, within AO353_03005 at 636.912 kb on - strand, within AO353_03005 at 637.004 kb on + strand, within AO353_03005 at 637.004 kb on + strand, within AO353_03005 at 637.005 kb on - strand, within AO353_03005 at 637.005 kb on - strand, within AO353_03005 at 637.005 kb on - strand, within AO353_03005 at 637.005 kb on - strand, within AO353_03005 at 637.139 kb on + strand, within AO353_03005 at 637.139 kb on + strand, within AO353_03005 at 637.140 kb on - strand, within AO353_03005 at 637.298 kb on + strand, within AO353_03005
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH7 remove 635,351 + AO353_02995 0.14 -1.4 635,352 - AO353_02995 0.14 +0.1 635,609 + AO353_02995 0.40 -0.1 635,609 + AO353_02995 0.40 -0.1 635,609 + AO353_02995 0.40 +0.6 635,610 - AO353_02995 0.40 +1.2 635,610 - AO353_02995 0.40 +0.7 635,610 - AO353_02995 0.40 -0.0 635,610 - AO353_02995 0.40 -0.1 635,610 - AO353_02995 0.40 +1.3 635,615 + AO353_02995 0.40 -1.2 635,616 - AO353_02995 0.40 +0.3 635,616 - AO353_02995 0.40 -1.1 635,726 + AO353_02995 0.51 +0.3 635,777 + AO353_02995 0.56 +1.9 635,778 - AO353_02995 0.56 +0.3 635,961 + AO353_02995 0.74 -0.4 635,961 + AO353_02995 0.74 -0.1 635,961 + AO353_02995 0.74 -0.2 635,961 + AO353_02995 0.74 -0.3 635,961 + AO353_02995 0.74 +1.7 635,961 + AO353_02995 0.74 -0.3 635,962 - AO353_02995 0.74 -0.4 635,962 - AO353_02995 0.74 -2.4 635,962 - AO353_02995 0.74 +0.6 636,017 - AO353_02995 0.80 +0.3 636,017 - AO353_02995 0.80 +0.6 636,219 + +0.1 636,219 + -0.9 636,219 + -0.0 636,219 + -0.1 636,219 + -0.1 636,219 + -0.1 636,219 + +0.9 636,220 - +0.6 636,220 - -1.6 636,313 + AO353_03000 0.28 +0.1 636,313 + AO353_03000 0.28 -2.0 636,314 - AO353_03000 0.28 -0.1 636,314 - AO353_03000 0.28 -1.8 636,322 - AO353_03000 0.31 -1.3 636,523 + -0.2 636,541 + -0.0 636,541 + +0.6 636,541 + +0.6 636,542 - +0.5 636,542 - -1.3 636,542 - +1.1 636,542 - -0.3 636,542 - -1.2 636,542 - +2.6 636,603 - -2.8 636,619 - +1.6 636,741 - AO353_03005 0.12 -0.7 636,741 - AO353_03005 0.12 -0.9 636,741 - AO353_03005 0.12 -0.4 636,741 - AO353_03005 0.12 -0.3 636,741 - AO353_03005 0.12 -0.9 636,741 - AO353_03005 0.12 +0.3 636,808 + AO353_03005 0.17 -1.4 636,911 + AO353_03005 0.24 -0.1 636,911 + AO353_03005 0.24 -1.4 636,911 + AO353_03005 0.24 -2.1 636,912 - AO353_03005 0.24 +0.7 637,004 + AO353_03005 0.31 -0.3 637,004 + AO353_03005 0.31 +0.9 637,005 - AO353_03005 0.31 +0.5 637,005 - AO353_03005 0.31 -2.3 637,005 - AO353_03005 0.31 -1.2 637,005 - AO353_03005 0.31 -0.3 637,139 + AO353_03005 0.41 +1.2 637,139 + AO353_03005 0.41 +0.1 637,140 - AO353_03005 0.41 -0.4 637,298 + AO353_03005 0.53 -0.4
Or see this region's nucleotide sequence