Experiment: Phosphomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2145 and Shew_2146 are separated by 489 nucleotides Shew_2146 and Shew_2147 are separated by 149 nucleotides Shew_2147 and Shew_2148 are separated by 229 nucleotides Shew_2148 and Shew_2149 are separated by 86 nucleotides
Shew_2145: Shew_2145 - MscS mechanosensitive ion channel (RefSeq), at 2,490,993 to 2,492,606
_2145
Shew_2146: Shew_2146 - hypothetical protein (RefSeq), at 2,493,096 to 2,493,269
_2146
Shew_2147: Shew_2147 - hypothetical protein (RefSeq), at 2,493,419 to 2,494,345
_2147
Shew_2148: Shew_2148 - hypothetical protein (RefSeq), at 2,494,575 to 2,494,982
_2148
Shew_2149: Shew_2149 - ABC transporter (RefSeq), at 2,495,069 to 2,495,929
_2149
Position (kb)
2493
2494
2495 Strain fitness (log2 ratio)
-2
-1
0
1 at 2492.428 kb on + strand, within Shew_2145 at 2492.669 kb on + strand at 2492.669 kb on + strand at 2492.677 kb on - strand at 2492.677 kb on - strand at 2493.218 kb on + strand, within Shew_2146 at 2493.280 kb on + strand at 2493.312 kb on - strand at 2494.195 kb on + strand, within Shew_2147 at 2494.311 kb on - strand at 2494.378 kb on + strand at 2494.386 kb on - strand at 2494.684 kb on - strand, within Shew_2148 at 2495.032 kb on + strand at 2495.186 kb on - strand, within Shew_2149
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.24 mg/ml remove 2,492,428 + Shew_2145 0.89 -1.0 2,492,669 + +1.0 2,492,669 + -0.9 2,492,677 - +0.3 2,492,677 - +0.4 2,493,218 + Shew_2146 0.70 -0.0 2,493,280 + +0.7 2,493,312 - -0.1 2,494,195 + Shew_2147 0.84 -0.9 2,494,311 - -2.1 2,494,378 + +0.8 2,494,386 - -0.5 2,494,684 - Shew_2148 0.27 -0.3 2,495,032 + +1.0 2,495,186 - Shew_2149 0.14 -1.1
Or see this region's nucleotide sequence