Experiment: Phosphomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1837 and Shew_1838 are separated by 90 nucleotides Shew_1838 and Shew_1839 are separated by 130 nucleotides Shew_1839 and Shew_1840 are separated by 14 nucleotides Shew_1840 and Shew_1841 are separated by 72 nucleotides
Shew_1837: Shew_1837 - hypothetical protein (RefSeq), at 2,114,231 to 2,115,361
_1837
Shew_1838: Shew_1838 - DNA replication initiation factor (RefSeq), at 2,115,452 to 2,116,162
_1838
Shew_1839: Shew_1839 - hypothetical protein (RefSeq), at 2,116,293 to 2,116,664
_1839
Shew_1840: Shew_1840 - arsenate reductase (RefSeq), at 2,116,679 to 2,117,041
_1840
Shew_1841: Shew_1841 - peptidase M48, Ste24p (RefSeq), at 2,117,114 to 2,118,571
_1841
Position (kb) 2115
2116
2117 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7 at 2114.457 kb on - strand, within Shew_1837 at 2115.446 kb on + strand at 2116.486 kb on + strand, within Shew_1839 at 2116.486 kb on + strand, within Shew_1839 at 2116.759 kb on + strand, within Shew_1840 at 2116.759 kb on + strand, within Shew_1840 at 2116.767 kb on - strand, within Shew_1840
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.24 mg/ml remove 2,114,457 - Shew_1837 0.20 +7.2 2,115,446 + +0.7 2,116,486 + Shew_1839 0.52 -0.9 2,116,486 + Shew_1839 0.52 +0.1 2,116,759 + Shew_1840 0.22 -0.9 2,116,759 + Shew_1840 0.22 -2.6 2,116,767 - Shew_1840 0.24 -0.6
Or see this region's nucleotide sequence