Experiment: Phosphomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1767 and Shew_1768 are separated by 418 nucleotides Shew_1768 and Shew_1769 are separated by 33 nucleotides Shew_1769 and Shew_1770 overlap by 4 nucleotides
Shew_1767: Shew_1767 - ATP-dependent helicase HrpA (RefSeq), at 2,019,461 to 2,023,348
_1767
Shew_1768: Shew_1768 - periplasmic nitrate reductase NapE (RefSeq), at 2,023,767 to 2,023,925
_1768
Shew_1769: Shew_1769 - NapD family protein (RefSeq), at 2,023,959 to 2,024,222
_1769
Shew_1770: Shew_1770 - nitrate reductase (RefSeq), at 2,024,219 to 2,026,714
_1770
Position (kb)
2023
2024 Strain fitness (log2 ratio)
-1
0
1 at 2022.783 kb on - strand, within Shew_1767 at 2022.838 kb on - strand, within Shew_1767 at 2022.838 kb on - strand, within Shew_1767 at 2022.840 kb on + strand, within Shew_1767 at 2023.763 kb on + strand at 2023.847 kb on - strand, within Shew_1768 at 2023.859 kb on - strand, within Shew_1768 at 2023.928 kb on - strand at 2023.961 kb on - strand at 2024.168 kb on + strand, within Shew_1769 at 2024.322 kb on + strand at 2024.520 kb on + strand, within Shew_1770 at 2024.817 kb on - strand, within Shew_1770
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.24 mg/ml remove 2,022,783 - Shew_1767 0.85 -1.5 2,022,838 - Shew_1767 0.87 +0.1 2,022,838 - Shew_1767 0.87 +0.6 2,022,840 + Shew_1767 0.87 +0.3 2,023,763 + +0.3 2,023,847 - Shew_1768 0.50 -0.6 2,023,859 - Shew_1768 0.58 -0.9 2,023,928 - -0.9 2,023,961 - -0.6 2,024,168 + Shew_1769 0.79 -0.7 2,024,322 + +0.2 2,024,520 + Shew_1770 0.12 +0.7 2,024,817 - Shew_1770 0.24 +0.4
Or see this region's nucleotide sequence