Experiment: Phosphomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1221 and Shew_1222 are separated by 363 nucleotides Shew_1222 and Shew_1223 are separated by 29 nucleotides Shew_1223 and Shew_1224 are separated by 132 nucleotides
Shew_1221: Shew_1221 - sodium ion-translocating decarboxylase, beta subunit (RefSeq), at 1,419,368 to 1,420,501
_1221
Shew_1222: Shew_1222 - hypothetical protein (RefSeq), at 1,420,865 to 1,421,050
_1222
Shew_1223: Shew_1223 - hypothetical protein (RefSeq), at 1,421,080 to 1,421,265
_1223
Shew_1224: Shew_1224 - sodium:dicarboxylate symporter (RefSeq), at 1,421,398 to 1,422,648
_1224
Position (kb) 1421
1422 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 1420.573 kb on + strand at 1420.581 kb on - strand at 1420.690 kb on - strand at 1420.975 kb on - strand, within Shew_1222 at 1421.139 kb on + strand, within Shew_1223 at 1421.575 kb on + strand, within Shew_1224 at 1421.705 kb on + strand, within Shew_1224 at 1421.713 kb on - strand, within Shew_1224 at 1422.135 kb on + strand, within Shew_1224
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.24 mg/ml remove 1,420,573 + -0.7 1,420,581 - +1.1 1,420,690 - +2.9 1,420,975 - Shew_1222 0.59 +0.3 1,421,139 + Shew_1223 0.32 -2.1 1,421,575 + Shew_1224 0.14 +4.9 1,421,705 + Shew_1224 0.25 +5.0 1,421,713 - Shew_1224 0.25 +4.9 1,422,135 + Shew_1224 0.59 +5.1
Or see this region's nucleotide sequence