Experiment: Phosphomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1135 and Shew_1136 are separated by 60 nucleotides Shew_1136 and Shew_1137 are separated by 81 nucleotides Shew_1137 and Shew_1138 are separated by 35 nucleotides
Shew_1135: Shew_1135 - protein of unknown function YGGT (RefSeq), at 1,310,024 to 1,310,572
_1135
Shew_1136: Shew_1136 - pyrroline-5-carboxylate reductase (EC 1.5.1.2) (from data) , at 1,310,633 to 1,311,451
_1136
Shew_1137: Shew_1137 - alanine racemase domain-containing protein (RefSeq), at 1,311,533 to 1,312,234
_1137
Shew_1138: Shew_1138 - twitching motility protein (RefSeq), at 1,312,270 to 1,313,307
_1138
Position (kb)
1311
1312
1313 Strain fitness (log2 ratio)
-2
-1
0
1 at 1310.666 kb on - strand at 1310.700 kb on - strand at 1310.849 kb on + strand, within Shew_1136 at 1311.110 kb on - strand, within Shew_1136 at 1311.303 kb on + strand, within Shew_1136 at 1311.378 kb on + strand at 1311.378 kb on + strand at 1311.378 kb on + strand at 1311.386 kb on - strand at 1311.518 kb on - strand at 1311.518 kb on - strand at 1311.530 kb on + strand at 1311.636 kb on - strand, within Shew_1137 at 1311.764 kb on - strand, within Shew_1137 at 1312.306 kb on - strand at 1312.500 kb on - strand, within Shew_1138 at 1312.531 kb on - strand, within Shew_1138 at 1312.531 kb on - strand, within Shew_1138 at 1312.773 kb on - strand, within Shew_1138 at 1312.821 kb on + strand, within Shew_1138 at 1313.169 kb on + strand, within Shew_1138 at 1313.213 kb on + strand at 1313.213 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.24 mg/ml remove 1,310,666 - -0.0 1,310,700 - -0.3 1,310,849 + Shew_1136 0.26 -1.1 1,311,110 - Shew_1136 0.58 -0.6 1,311,303 + Shew_1136 0.82 -1.0 1,311,378 + -0.5 1,311,378 + +0.8 1,311,378 + -0.1 1,311,386 - +0.4 1,311,518 - +0.6 1,311,518 - +0.1 1,311,530 + +0.2 1,311,636 - Shew_1137 0.15 +0.9 1,311,764 - Shew_1137 0.33 -0.2 1,312,306 - -1.1 1,312,500 - Shew_1138 0.22 -1.8 1,312,531 - Shew_1138 0.25 -2.3 1,312,531 - Shew_1138 0.25 -2.3 1,312,773 - Shew_1138 0.48 -1.3 1,312,821 + Shew_1138 0.53 -2.4 1,313,169 + Shew_1138 0.87 -2.1 1,313,213 + -1.7 1,313,213 + -1.5
Or see this region's nucleotide sequence