Experiment: Phosphomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1132 and Shew_1133 are separated by 69 nucleotides Shew_1133 and Shew_1134 are separated by 86 nucleotides Shew_1134 and Shew_1135 are separated by 0 nucleotides Shew_1135 and Shew_1136 are separated by 60 nucleotides
Shew_1132: Shew_1132 - RdgB/HAM1 family non-canonical purine NTP pyrophosphatase (RefSeq), at 1,308,471 to 1,309,142
_1132
Shew_1133: Shew_1133 - hypothetical protein (RefSeq), at 1,309,212 to 1,309,646
_1133
Shew_1134: Shew_1134 - hypothetical protein (RefSeq), at 1,309,733 to 1,310,023
_1134
Shew_1135: Shew_1135 - protein of unknown function YGGT (RefSeq), at 1,310,024 to 1,310,572
_1135
Shew_1136: Shew_1136 - pyrroline-5-carboxylate reductase (EC 1.5.1.2) (from data) , at 1,310,633 to 1,311,451
_1136
Position (kb)
1309
1310
1311 Strain fitness (log2 ratio)
-2
-1
0
1 at 1309.196 kb on + strand at 1309.249 kb on + strand at 1309.367 kb on + strand, within Shew_1133 at 1309.375 kb on - strand, within Shew_1133 at 1309.536 kb on - strand, within Shew_1133 at 1309.546 kb on - strand, within Shew_1133 at 1309.701 kb on - strand at 1309.739 kb on + strand at 1309.747 kb on - strand at 1309.850 kb on - strand, within Shew_1134 at 1309.918 kb on + strand, within Shew_1134 at 1309.918 kb on + strand, within Shew_1134 at 1309.918 kb on + strand, within Shew_1134 at 1309.970 kb on - strand, within Shew_1134 at 1310.666 kb on - strand at 1310.700 kb on - strand at 1310.849 kb on + strand, within Shew_1136
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.24 mg/ml remove 1,309,196 + +0.2 1,309,249 + +0.5 1,309,367 + Shew_1133 0.36 +0.9 1,309,375 - Shew_1133 0.37 +0.3 1,309,536 - Shew_1133 0.74 +0.4 1,309,546 - Shew_1133 0.77 -2.2 1,309,701 - +0.9 1,309,739 + +0.8 1,309,747 - -0.2 1,309,850 - Shew_1134 0.40 -0.6 1,309,918 + Shew_1134 0.64 +0.3 1,309,918 + Shew_1134 0.64 +0.6 1,309,918 + Shew_1134 0.64 -0.4 1,309,970 - Shew_1134 0.81 +0.8 1,310,666 - -0.0 1,310,700 - -0.3 1,310,849 + Shew_1136 0.26 -1.1
Or see this region's nucleotide sequence