Experiment: Phosphomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gltD and gltB are separated by 5 nucleotides gltB and Shew_1033 are separated by 430 nucleotides
Shew_1031: gltD - glutamate synthase subunit beta (RefSeq), at 1,189,496 to 1,190,905
gltD
Shew_1032: gltB - glutamate synthase subunit alpha (RefSeq), at 1,190,911 to 1,195,359
gltB
Shew_1033: Shew_1033 - putative radical SAM protein (RefSeq), at 1,195,790 to 1,196,782
_1033
Position (kb)
1190
1191
1192
1193
1194
1195
1196 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1190.009 kb on + strand, within gltD at 1190.009 kb on + strand, within gltD at 1190.017 kb on - strand, within gltD at 1190.017 kb on - strand, within gltD at 1190.065 kb on + strand, within gltD at 1190.090 kb on + strand, within gltD at 1190.266 kb on + strand, within gltD at 1190.327 kb on - strand, within gltD at 1190.706 kb on - strand, within gltD at 1190.745 kb on + strand, within gltD at 1190.745 kb on + strand, within gltD at 1190.798 kb on - strand at 1191.083 kb on + strand at 1191.091 kb on - strand at 1191.091 kb on - strand at 1191.251 kb on + strand at 1191.318 kb on - strand at 1191.352 kb on - strand at 1191.391 kb on - strand, within gltB at 1191.457 kb on + strand, within gltB at 1191.535 kb on + strand, within gltB at 1191.755 kb on - strand, within gltB at 1191.950 kb on + strand, within gltB at 1192.182 kb on + strand, within gltB at 1192.214 kb on + strand, within gltB at 1192.342 kb on + strand, within gltB at 1192.344 kb on - strand, within gltB at 1192.487 kb on + strand, within gltB at 1192.589 kb on + strand, within gltB at 1192.749 kb on + strand, within gltB at 1192.933 kb on - strand, within gltB at 1192.984 kb on + strand, within gltB at 1193.116 kb on + strand, within gltB at 1193.233 kb on - strand, within gltB at 1193.272 kb on + strand, within gltB at 1193.344 kb on - strand, within gltB at 1193.464 kb on + strand, within gltB at 1193.527 kb on - strand, within gltB at 1193.615 kb on - strand, within gltB at 1194.030 kb on - strand, within gltB at 1194.068 kb on - strand, within gltB at 1194.313 kb on + strand, within gltB at 1194.331 kb on + strand, within gltB at 1194.403 kb on - strand, within gltB at 1194.441 kb on + strand, within gltB at 1194.471 kb on - strand, within gltB at 1194.519 kb on + strand, within gltB at 1194.851 kb on + strand, within gltB at 1194.859 kb on - strand, within gltB at 1194.939 kb on - strand at 1194.984 kb on + strand at 1195.706 kb on + strand at 1195.860 kb on + strand at 1195.977 kb on + strand, within Shew_1033 at 1196.029 kb on + strand, within Shew_1033 at 1196.071 kb on + strand, within Shew_1033 at 1196.126 kb on - strand, within Shew_1033 at 1196.178 kb on + strand, within Shew_1033 at 1196.267 kb on - strand, within Shew_1033 at 1196.307 kb on + strand, within Shew_1033
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.24 mg/ml remove 1,190,009 + gltD Shew_1031 0.36 +0.8 1,190,009 + gltD Shew_1031 0.36 +0.6 1,190,017 - gltD Shew_1031 0.37 +0.3 1,190,017 - gltD Shew_1031 0.37 +0.6 1,190,065 + gltD Shew_1031 0.40 -0.3 1,190,090 + gltD Shew_1031 0.42 +0.4 1,190,266 + gltD Shew_1031 0.55 +0.1 1,190,327 - gltD Shew_1031 0.59 +1.4 1,190,706 - gltD Shew_1031 0.86 +1.4 1,190,745 + gltD Shew_1031 0.89 +1.7 1,190,745 + gltD Shew_1031 0.89 -0.0 1,190,798 - +1.1 1,191,083 + +0.3 1,191,091 - +0.9 1,191,091 - +0.5 1,191,251 + +0.3 1,191,318 - +0.3 1,191,352 - +0.5 1,191,391 - gltB Shew_1032 0.11 +0.9 1,191,457 + gltB Shew_1032 0.12 -0.1 1,191,535 + gltB Shew_1032 0.14 -0.4 1,191,755 - gltB Shew_1032 0.19 +0.1 1,191,950 + gltB Shew_1032 0.23 +0.3 1,192,182 + gltB Shew_1032 0.29 +0.3 1,192,214 + gltB Shew_1032 0.29 +0.0 1,192,342 + gltB Shew_1032 0.32 -0.9 1,192,344 - gltB Shew_1032 0.32 -0.2 1,192,487 + gltB Shew_1032 0.35 +0.8 1,192,589 + gltB Shew_1032 0.38 -0.5 1,192,749 + gltB Shew_1032 0.41 +0.0 1,192,933 - gltB Shew_1032 0.45 +0.2 1,192,984 + gltB Shew_1032 0.47 -0.5 1,193,116 + gltB Shew_1032 0.50 +0.3 1,193,233 - gltB Shew_1032 0.52 -1.9 1,193,272 + gltB Shew_1032 0.53 -0.5 1,193,344 - gltB Shew_1032 0.55 +0.7 1,193,464 + gltB Shew_1032 0.57 -0.8 1,193,527 - gltB Shew_1032 0.59 -0.1 1,193,615 - gltB Shew_1032 0.61 +0.7 1,194,030 - gltB Shew_1032 0.70 +0.3 1,194,068 - gltB Shew_1032 0.71 +0.8 1,194,313 + gltB Shew_1032 0.76 +0.8 1,194,331 + gltB Shew_1032 0.77 +0.2 1,194,403 - gltB Shew_1032 0.78 +0.4 1,194,441 + gltB Shew_1032 0.79 +0.3 1,194,471 - gltB Shew_1032 0.80 +1.6 1,194,519 + gltB Shew_1032 0.81 +1.2 1,194,851 + gltB Shew_1032 0.89 -1.1 1,194,859 - gltB Shew_1032 0.89 +0.1 1,194,939 - -2.1 1,194,984 + -1.5 1,195,706 + -2.1 1,195,860 + +0.3 1,195,977 + Shew_1033 0.19 +0.0 1,196,029 + Shew_1033 0.24 -0.2 1,196,071 + Shew_1033 0.28 -2.8 1,196,126 - Shew_1033 0.34 -1.9 1,196,178 + Shew_1033 0.39 -0.8 1,196,267 - Shew_1033 0.48 +0.4 1,196,307 + Shew_1033 0.52 -1.5
Or see this region's nucleotide sequence