Experiment: Phosphomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1015 and Shew_1016 are separated by 75 nucleotides Shew_1016 and Shew_1017 overlap by 8 nucleotides Shew_1017 and Shew_1018 are separated by 128 nucleotides Shew_1018 and Shew_1019 are separated by 60 nucleotides
Shew_1015: Shew_1015 - Cl- channel, voltage-gated family protein (RefSeq), at 1,174,427 to 1,176,136
_1015
Shew_1016: Shew_1016 - hypothetical protein (RefSeq), at 1,176,212 to 1,177,129
_1016
Shew_1017: Shew_1017 - iron-sulfur cluster insertion protein ErpA (RefSeq), at 1,177,122 to 1,177,472
_1017
Shew_1018: Shew_1018 - hypothetical protein (RefSeq), at 1,177,601 to 1,178,074
_1018
Shew_1019: Shew_1019 - hypothetical protein (RefSeq), at 1,178,135 to 1,178,521
_1019
Position (kb) 1177
1178 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 1176.829 kb on + strand, within Shew_1016 at 1176.854 kb on - strand, within Shew_1016 at 1176.895 kb on - strand, within Shew_1016 at 1176.983 kb on - strand, within Shew_1016 at 1177.713 kb on - strand, within Shew_1018 at 1177.744 kb on + strand, within Shew_1018 at 1178.060 kb on - strand at 1178.154 kb on - strand at 1178.298 kb on - strand, within Shew_1019 at 1178.438 kb on - strand, within Shew_1019
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.24 mg/ml remove 1,176,829 + Shew_1016 0.67 -3.7 1,176,854 - Shew_1016 0.70 -3.3 1,176,895 - Shew_1016 0.74 -5.6 1,176,983 - Shew_1016 0.84 +3.1 1,177,713 - Shew_1018 0.24 -0.8 1,177,744 + Shew_1018 0.30 -0.1 1,178,060 - -1.3 1,178,154 - +0.7 1,178,298 - Shew_1019 0.42 -0.2 1,178,438 - Shew_1019 0.78 -1.0
Or see this region's nucleotide sequence