Experiment: Phosphomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0400 and Shew_0401 overlap by 11 nucleotides Shew_0401 and Shew_0402 overlap by 8 nucleotides Shew_0402 and Shew_0403 are separated by 165 nucleotides
Shew_0400: Shew_0400 - methylation site containing protein (RefSeq), at 448,909 to 449,706
_0400
Shew_0401: Shew_0401 - MSHA biogenesis protein MshP (RefSeq), at 449,696 to 450,133
_0401
Shew_0402: Shew_0402 - MSHA biogenesis protein MshQ (RefSeq), at 450,126 to 452,828
_0402
Shew_0403: Shew_0403 - cell shape determining protein MreB (RefSeq), at 452,994 to 454,043
_0403
Position (kb)
450
451
452
453 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 449.252 kb on + strand, within Shew_0400 at 449.313 kb on - strand, within Shew_0400 at 449.363 kb on - strand, within Shew_0400 at 449.418 kb on - strand, within Shew_0400 at 449.418 kb on - strand, within Shew_0400 at 449.642 kb on + strand at 449.989 kb on - strand, within Shew_0401 at 450.014 kb on + strand, within Shew_0401 at 450.170 kb on + strand at 450.321 kb on - strand at 451.130 kb on + strand, within Shew_0402 at 451.583 kb on + strand, within Shew_0402 at 451.583 kb on + strand, within Shew_0402 at 451.591 kb on - strand, within Shew_0402 at 451.643 kb on + strand, within Shew_0402 at 451.784 kb on - strand, within Shew_0402 at 451.814 kb on + strand, within Shew_0402 at 451.814 kb on + strand, within Shew_0402 at 451.852 kb on + strand, within Shew_0402 at 451.884 kb on + strand, within Shew_0402 at 451.884 kb on + strand, within Shew_0402 at 451.884 kb on + strand, within Shew_0402 at 451.884 kb on + strand, within Shew_0402 at 451.884 kb on + strand, within Shew_0402 at 452.080 kb on + strand, within Shew_0402 at 452.088 kb on - strand, within Shew_0402 at 452.536 kb on + strand, within Shew_0402 at 452.549 kb on - strand, within Shew_0402 at 452.680 kb on + strand at 452.747 kb on - strand at 452.859 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.24 mg/ml remove 449,252 + Shew_0400 0.43 +1.6 449,313 - Shew_0400 0.51 -1.9 449,363 - Shew_0400 0.57 +1.1 449,418 - Shew_0400 0.64 +0.8 449,418 - Shew_0400 0.64 +1.4 449,642 + +1.7 449,989 - Shew_0401 0.67 -1.2 450,014 + Shew_0401 0.73 +0.0 450,170 + +1.4 450,321 - +0.1 451,130 + Shew_0402 0.37 +1.4 451,583 + Shew_0402 0.54 +1.3 451,583 + Shew_0402 0.54 +1.2 451,591 - Shew_0402 0.54 +1.8 451,643 + Shew_0402 0.56 +0.8 451,784 - Shew_0402 0.61 +2.5 451,814 + Shew_0402 0.62 +2.9 451,814 + Shew_0402 0.62 +1.4 451,852 + Shew_0402 0.64 +1.2 451,884 + Shew_0402 0.65 -4.1 451,884 + Shew_0402 0.65 +3.0 451,884 + Shew_0402 0.65 +0.8 451,884 + Shew_0402 0.65 +2.0 451,884 + Shew_0402 0.65 +0.2 452,080 + Shew_0402 0.72 +1.5 452,088 - Shew_0402 0.73 +0.5 452,536 + Shew_0402 0.89 +1.4 452,549 - Shew_0402 0.90 +0.7 452,680 + +1.0 452,747 - +1.4 452,859 - -0.3
Or see this region's nucleotide sequence