Experiment: Phosphomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0297 and Shew_0298 are separated by 125 nucleotides Shew_0298 and Shew_0299 are separated by 164 nucleotides Shew_0299 and Shew_0300 are separated by 130 nucleotides
Shew_0297: Shew_0297 - hypothetical protein (RefSeq), at 317,928 to 319,835
_0297
Shew_0298: Shew_0298 - Zn-dependent hydrolase of the beta-lactamase fold (RefSeq), at 319,961 to 321,049
_0298
Shew_0299: Shew_0299 - TetR family transcriptional regulator (RefSeq), at 321,214 to 321,822
_0299
Shew_0300: Shew_0300 - sodium:neurotransmitter symporter (RefSeq), at 321,953 to 323,311
_0300
Position (kb) 319
320
321
322 Strain fitness (log2 ratio) -2
-1
0
1 at 319.211 kb on - strand, within Shew_0297 at 319.325 kb on + strand, within Shew_0297 at 319.541 kb on + strand, within Shew_0297 at 319.541 kb on + strand, within Shew_0297 at 319.977 kb on + strand at 320.184 kb on - strand, within Shew_0298 at 320.208 kb on - strand, within Shew_0298 at 320.230 kb on - strand, within Shew_0298 at 320.337 kb on - strand, within Shew_0298 at 320.463 kb on + strand, within Shew_0298 at 320.513 kb on + strand, within Shew_0298 at 320.773 kb on - strand, within Shew_0298 at 320.778 kb on + strand, within Shew_0298 at 320.791 kb on - strand, within Shew_0298 at 320.960 kb on - strand at 321.034 kb on + strand at 321.251 kb on - strand at 321.387 kb on + strand, within Shew_0299 at 321.528 kb on - strand, within Shew_0299 at 321.629 kb on + strand, within Shew_0299 at 321.725 kb on + strand, within Shew_0299 at 321.762 kb on + strand at 321.782 kb on + strand at 321.825 kb on - strand at 321.888 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.24 mg/ml remove 319,211 - Shew_0297 0.67 -0.8 319,325 + Shew_0297 0.73 +0.2 319,541 + Shew_0297 0.85 -1.8 319,541 + Shew_0297 0.85 -1.7 319,977 + +0.0 320,184 - Shew_0298 0.20 +0.3 320,208 - Shew_0298 0.23 +0.3 320,230 - Shew_0298 0.25 +0.2 320,337 - Shew_0298 0.35 +0.0 320,463 + Shew_0298 0.46 +0.7 320,513 + Shew_0298 0.51 +0.5 320,773 - Shew_0298 0.75 +0.1 320,778 + Shew_0298 0.75 -0.4 320,791 - Shew_0298 0.76 +0.2 320,960 - -0.7 321,034 + -1.0 321,251 - +1.4 321,387 + Shew_0299 0.28 +0.5 321,528 - Shew_0299 0.52 -0.0 321,629 + Shew_0299 0.68 +0.2 321,725 + Shew_0299 0.84 -0.5 321,762 + +0.2 321,782 + -0.1 321,825 - +1.6 321,888 - +0.2
Or see this region's nucleotide sequence