Experiment: Phosphomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0061 and Shew_0062 are separated by 491 nucleotides Shew_0062 and Shew_0063 are separated by 17 nucleotides Shew_0063 and Shew_0064 are separated by 20 nucleotides Shew_0064 and Shew_0065 are separated by 68 nucleotides
Shew_0061: Shew_0061 - formate dehydrogenase, gamma subunit (RefSeq), at 73,390 to 74,397
_0061
Shew_0062: Shew_0062 - hypothetical protein (RefSeq), at 74,889 to 75,086
_0062
Shew_0063: Shew_0063 - molybdopterin oxidoreductase (RefSeq), at 75,104 to 77,959
_0063
Shew_0064: Shew_0064 - 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein (RefSeq), at 77,980 to 78,549
_0064
Shew_0065: Shew_0065 - formate dehydrogenase, gamma subunit (RefSeq), at 78,618 to 79,610
_0065
Position (kb) 75
76
77
78 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 74.119 kb on - strand, within Shew_0061 at 74.119 kb on - strand, within Shew_0061 at 74.130 kb on + strand, within Shew_0061 at 74.295 kb on - strand, within Shew_0061 at 74.330 kb on - strand at 74.330 kb on - strand at 74.332 kb on + strand at 74.332 kb on + strand at 74.332 kb on + strand at 74.340 kb on - strand at 74.340 kb on - strand at 74.399 kb on - strand at 74.642 kb on + strand at 74.653 kb on + strand at 74.653 kb on + strand at 74.661 kb on - strand at 74.661 kb on - strand at 75.419 kb on - strand, within Shew_0063 at 75.712 kb on + strand, within Shew_0063 at 75.718 kb on + strand, within Shew_0063 at 75.726 kb on - strand, within Shew_0063 at 75.726 kb on - strand, within Shew_0063 at 75.897 kb on + strand, within Shew_0063 at 75.905 kb on - strand, within Shew_0063 at 75.909 kb on + strand, within Shew_0063 at 75.910 kb on - strand, within Shew_0063 at 76.063 kb on - strand, within Shew_0063 at 76.072 kb on + strand, within Shew_0063 at 76.086 kb on + strand, within Shew_0063 at 76.488 kb on + strand, within Shew_0063 at 76.574 kb on + strand, within Shew_0063 at 76.719 kb on - strand, within Shew_0063 at 76.719 kb on - strand, within Shew_0063 at 76.932 kb on + strand, within Shew_0063 at 76.994 kb on + strand, within Shew_0063 at 77.047 kb on + strand, within Shew_0063 at 77.060 kb on - strand, within Shew_0063 at 77.060 kb on - strand, within Shew_0063 at 77.060 kb on - strand, within Shew_0063 at 77.083 kb on - strand, within Shew_0063 at 77.156 kb on + strand, within Shew_0063 at 77.164 kb on - strand, within Shew_0063 at 77.256 kb on - strand, within Shew_0063 at 77.370 kb on + strand, within Shew_0063 at 77.608 kb on + strand, within Shew_0063 at 77.621 kb on - strand, within Shew_0063 at 77.751 kb on - strand at 77.794 kb on - strand at 77.882 kb on + strand at 77.928 kb on + strand at 78.067 kb on + strand, within Shew_0064 at 78.075 kb on - strand, within Shew_0064 at 78.138 kb on - strand, within Shew_0064 at 78.265 kb on + strand, within Shew_0064 at 78.265 kb on + strand, within Shew_0064 at 78.372 kb on - strand, within Shew_0064 at 78.519 kb on - strand at 78.568 kb on - strand at 78.579 kb on - strand at 78.712 kb on + strand at 78.817 kb on + strand, within Shew_0065 at 78.853 kb on + strand, within Shew_0065
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.24 mg/ml remove 74,119 - Shew_0061 0.72 -2.7 74,119 - Shew_0061 0.72 -0.1 74,130 + Shew_0061 0.73 -0.1 74,295 - Shew_0061 0.90 -0.1 74,330 - +0.0 74,330 - +0.3 74,332 + -0.9 74,332 + -0.5 74,332 + -0.5 74,340 - -0.4 74,340 - -0.4 74,399 - -1.3 74,642 + -0.5 74,653 + +0.2 74,653 + +0.2 74,661 - -2.4 74,661 - +0.1 75,419 - Shew_0063 0.11 -0.6 75,712 + Shew_0063 0.21 -0.1 75,718 + Shew_0063 0.21 -0.0 75,726 - Shew_0063 0.22 -0.1 75,726 - Shew_0063 0.22 +1.5 75,897 + Shew_0063 0.28 +2.0 75,905 - Shew_0063 0.28 -3.6 75,909 + Shew_0063 0.28 +0.9 75,910 - Shew_0063 0.28 +0.9 76,063 - Shew_0063 0.34 +0.2 76,072 + Shew_0063 0.34 +0.1 76,086 + Shew_0063 0.34 +0.0 76,488 + Shew_0063 0.48 -3.1 76,574 + Shew_0063 0.51 -0.0 76,719 - Shew_0063 0.57 -1.1 76,719 - Shew_0063 0.57 -0.1 76,932 + Shew_0063 0.64 -2.6 76,994 + Shew_0063 0.66 +0.2 77,047 + Shew_0063 0.68 -0.2 77,060 - Shew_0063 0.68 +0.4 77,060 - Shew_0063 0.68 +0.4 77,060 - Shew_0063 0.68 +2.0 77,083 - Shew_0063 0.69 +0.2 77,156 + Shew_0063 0.72 +0.2 77,164 - Shew_0063 0.72 +0.1 77,256 - Shew_0063 0.75 -0.5 77,370 + Shew_0063 0.79 +0.3 77,608 + Shew_0063 0.88 -0.6 77,621 - Shew_0063 0.88 +0.3 77,751 - -0.4 77,794 - +0.1 77,882 + +0.7 77,928 + +0.6 78,067 + Shew_0064 0.15 -0.1 78,075 - Shew_0064 0.17 +1.4 78,138 - Shew_0064 0.28 -0.9 78,265 + Shew_0064 0.50 +0.5 78,265 + Shew_0064 0.50 -0.3 78,372 - Shew_0064 0.69 +0.7 78,519 - -0.9 78,568 - -2.3 78,579 - +0.1 78,712 + -0.2 78,817 + Shew_0065 0.20 -0.4 78,853 + Shew_0065 0.24 -0.1
Or see this region's nucleotide sequence