Experiment: Phosphomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0014 and Shew_0015 are separated by 165 nucleotides Shew_0015 and Shew_0016 are separated by 133 nucleotides Shew_0016 and Shew_0017 overlap by 17 nucleotides Shew_0017 and fadA are separated by 354 nucleotides
Shew_0014: Shew_0014 - hypothetical protein (RefSeq), at 17,578 to 18,018
_0014
Shew_0015: Shew_0015 - SirA family protein (RefSeq), at 18,184 to 18,429
_0015
Shew_0016: Shew_0016 - ybaK/ebsC protein (RefSeq), at 18,563 to 19,027
_0016
Shew_0017: Shew_0017 - GCN5-related N-acetyltransferase (RefSeq), at 19,011 to 19,499
_0017
Shew_0018: fadA - 3-ketoacyl-CoA thiolase (RefSeq), at 19,854 to 21,017
fadA
Position (kb) 18
19
20 Strain fitness (log2 ratio) -2
-1
0
1 at 17.731 kb on - strand, within Shew_0014 at 17.744 kb on - strand, within Shew_0014 at 18.105 kb on + strand at 18.144 kb on - strand at 18.181 kb on + strand at 19.024 kb on + strand at 19.075 kb on + strand, within Shew_0017 at 19.170 kb on + strand, within Shew_0017 at 19.334 kb on + strand, within Shew_0017 at 19.339 kb on + strand, within Shew_0017 at 19.346 kb on + strand, within Shew_0017 at 19.347 kb on - strand, within Shew_0017 at 19.426 kb on + strand, within Shew_0017 at 19.771 kb on - strand at 19.871 kb on - strand at 19.900 kb on - strand at 19.960 kb on - strand at 19.961 kb on + strand at 19.965 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.24 mg/ml remove 17,731 - Shew_0014 0.35 -0.2 17,744 - Shew_0014 0.38 -0.7 18,105 + -0.8 18,144 - +0.7 18,181 + +0.3 19,024 + -0.2 19,075 + Shew_0017 0.13 -0.4 19,170 + Shew_0017 0.33 -0.9 19,334 + Shew_0017 0.66 +0.1 19,339 + Shew_0017 0.67 -0.0 19,346 + Shew_0017 0.69 -0.4 19,347 - Shew_0017 0.69 -2.1 19,426 + Shew_0017 0.85 +1.1 19,771 - -1.6 19,871 - -0.1 19,900 - -0.3 19,960 - -2.0 19,961 + +0.1 19,965 - +0.3
Or see this region's nucleotide sequence