Experiment: Phosphomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0011 and Shew_0012 are separated by 5 nucleotides Shew_0012 and Shew_0013 are separated by 212 nucleotides Shew_0013 and Shew_0014 are separated by 302 nucleotides Shew_0014 and Shew_0015 are separated by 165 nucleotides
Shew_0011: Shew_0011 - DNA-3-methyladenine glycosylase I (RefSeq), at 12,604 to 13,173
_0011
Shew_0012: Shew_0012 - hypothetical protein (RefSeq), at 13,179 to 13,859
_0012
Shew_0013: Shew_0013 - amidohydrolase (RefSeq), at 14,072 to 17,275
_0013
Shew_0014: Shew_0014 - hypothetical protein (RefSeq), at 17,578 to 18,018
_0014
Shew_0015: Shew_0015 - SirA family protein (RefSeq), at 18,184 to 18,429
_0015
Position (kb) 14
15
16
17
18 Strain fitness (log2 ratio) -2
-1
0
1 at 13.201 kb on + strand at 13.245 kb on - strand at 13.247 kb on + strand, within Shew_0012 at 13.584 kb on - strand, within Shew_0012 at 13.675 kb on + strand, within Shew_0012 at 13.726 kb on + strand, within Shew_0012 at 13.726 kb on + strand, within Shew_0012 at 13.726 kb on + strand, within Shew_0012 at 13.726 kb on + strand, within Shew_0012 at 13.734 kb on - strand, within Shew_0012 at 13.734 kb on - strand, within Shew_0012 at 13.734 kb on - strand, within Shew_0012 at 13.786 kb on - strand, within Shew_0012 at 13.876 kb on + strand at 13.882 kb on - strand at 14.171 kb on - strand at 14.311 kb on + strand at 14.453 kb on + strand, within Shew_0013 at 14.490 kb on + strand, within Shew_0013 at 14.498 kb on - strand, within Shew_0013 at 14.530 kb on + strand, within Shew_0013 at 14.719 kb on + strand, within Shew_0013 at 14.726 kb on - strand, within Shew_0013 at 14.815 kb on + strand, within Shew_0013 at 14.830 kb on + strand, within Shew_0013 at 14.973 kb on + strand, within Shew_0013 at 14.973 kb on + strand, within Shew_0013 at 14.978 kb on + strand, within Shew_0013 at 14.981 kb on - strand, within Shew_0013 at 14.991 kb on - strand, within Shew_0013 at 15.002 kb on - strand, within Shew_0013 at 15.012 kb on - strand, within Shew_0013 at 15.065 kb on - strand, within Shew_0013 at 15.260 kb on - strand, within Shew_0013 at 15.262 kb on + strand, within Shew_0013 at 15.360 kb on + strand, within Shew_0013 at 15.426 kb on + strand, within Shew_0013 at 15.633 kb on + strand, within Shew_0013 at 15.680 kb on + strand, within Shew_0013 at 16.026 kb on + strand, within Shew_0013 at 16.275 kb on + strand, within Shew_0013 at 16.337 kb on + strand, within Shew_0013 at 16.444 kb on + strand, within Shew_0013 at 16.501 kb on + strand, within Shew_0013 at 16.616 kb on + strand, within Shew_0013 at 16.625 kb on - strand, within Shew_0013 at 16.641 kb on - strand, within Shew_0013 at 16.696 kb on + strand, within Shew_0013 at 16.765 kb on + strand, within Shew_0013 at 16.779 kb on - strand, within Shew_0013 at 17.000 kb on + strand at 17.118 kb on + strand at 17.126 kb on - strand at 17.236 kb on - strand at 17.731 kb on - strand, within Shew_0014 at 17.744 kb on - strand, within Shew_0014 at 18.105 kb on + strand at 18.144 kb on - strand at 18.181 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.24 mg/ml remove 13,201 + -1.3 13,245 - +0.3 13,247 + Shew_0012 0.10 +0.6 13,584 - Shew_0012 0.59 -0.6 13,675 + Shew_0012 0.73 -0.9 13,726 + Shew_0012 0.80 +0.4 13,726 + Shew_0012 0.80 +1.5 13,726 + Shew_0012 0.80 +0.3 13,726 + Shew_0012 0.80 +0.2 13,734 - Shew_0012 0.81 -0.9 13,734 - Shew_0012 0.81 -0.1 13,734 - Shew_0012 0.81 -0.6 13,786 - Shew_0012 0.89 -0.5 13,876 + -0.0 13,882 - -0.0 14,171 - -0.1 14,311 + +0.5 14,453 + Shew_0013 0.12 +0.1 14,490 + Shew_0013 0.13 -0.5 14,498 - Shew_0013 0.13 -0.4 14,530 + Shew_0013 0.14 -1.5 14,719 + Shew_0013 0.20 -0.2 14,726 - Shew_0013 0.20 -2.0 14,815 + Shew_0013 0.23 -0.2 14,830 + Shew_0013 0.24 -0.7 14,973 + Shew_0013 0.28 -1.8 14,973 + Shew_0013 0.28 +0.3 14,978 + Shew_0013 0.28 -0.1 14,981 - Shew_0013 0.28 +0.3 14,991 - Shew_0013 0.29 +0.0 15,002 - Shew_0013 0.29 +0.6 15,012 - Shew_0013 0.29 +1.5 15,065 - Shew_0013 0.31 -0.5 15,260 - Shew_0013 0.37 -2.7 15,262 + Shew_0013 0.37 +0.6 15,360 + Shew_0013 0.40 +0.4 15,426 + Shew_0013 0.42 +0.1 15,633 + Shew_0013 0.49 -0.3 15,680 + Shew_0013 0.50 -0.6 16,026 + Shew_0013 0.61 +0.0 16,275 + Shew_0013 0.69 -1.4 16,337 + Shew_0013 0.71 +0.4 16,444 + Shew_0013 0.74 +0.1 16,501 + Shew_0013 0.76 -0.1 16,616 + Shew_0013 0.79 +0.6 16,625 - Shew_0013 0.80 -0.5 16,641 - Shew_0013 0.80 +0.1 16,696 + Shew_0013 0.82 +0.0 16,765 + Shew_0013 0.84 +1.0 16,779 - Shew_0013 0.84 +1.2 17,000 + -0.0 17,118 + +0.7 17,126 - -0.9 17,236 - +0.1 17,731 - Shew_0014 0.35 -0.2 17,744 - Shew_0014 0.38 -0.7 18,105 + -0.8 18,144 - +0.7 18,181 + +0.3
Or see this region's nucleotide sequence