Experiment: Succinic Acid; Light intensity 18 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3397 and Rru_A3398 are separated by 98 nucleotides Rru_A3398 and Rru_A3399 are separated by 244 nucleotides Rru_A3399 and Rru_A3400 are separated by 222 nucleotides
Rru_A3397: Rru_A3397 - hypothetical protein (NCBI), at 3,914,148 to 3,914,399
_A3397
Rru_A3398: Rru_A3398 - Transcriptional Regulator, XRE family (NCBI), at 3,914,498 to 3,915,040
_A3398
Rru_A3399: Rru_A3399 - Transcriptional Regulator, BadM/Rrf2 family (NCBI), at 3,915,285 to 3,915,767
_A3399
Rru_A3400: Rru_A3400 - Thiosulphate-binding protein (NCBI), at 3,915,990 to 3,917,054
_A3400
Position (kb)
3915
3916 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 3914.580 kb on + strand, within Rru_A3398 at 3914.581 kb on - strand, within Rru_A3398 at 3914.945 kb on - strand, within Rru_A3398 at 3915.057 kb on - strand at 3915.121 kb on + strand at 3915.249 kb on - strand at 3915.258 kb on - strand at 3915.289 kb on + strand at 3915.290 kb on - strand at 3915.306 kb on + strand at 3915.386 kb on + strand, within Rru_A3399 at 3915.386 kb on + strand, within Rru_A3399 at 3915.387 kb on - strand, within Rru_A3399 at 3915.472 kb on - strand, within Rru_A3399 at 3915.498 kb on - strand, within Rru_A3399 at 3915.498 kb on - strand, within Rru_A3399 at 3915.636 kb on + strand, within Rru_A3399 at 3915.798 kb on - strand at 3915.910 kb on + strand at 3915.910 kb on + strand at 3915.911 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 18 uM remove 3,914,580 + Rru_A3398 0.15 -5.9 3,914,581 - Rru_A3398 0.15 +2.2 3,914,945 - Rru_A3398 0.82 +0.8 3,915,057 - +0.9 3,915,121 + +1.4 3,915,249 - -2.3 3,915,258 - -0.6 3,915,289 + +0.1 3,915,290 - +2.3 3,915,306 + -4.6 3,915,386 + Rru_A3399 0.21 -1.9 3,915,386 + Rru_A3399 0.21 +0.1 3,915,387 - Rru_A3399 0.21 -1.8 3,915,472 - Rru_A3399 0.39 +0.6 3,915,498 - Rru_A3399 0.44 +0.1 3,915,498 - Rru_A3399 0.44 +0.1 3,915,636 + Rru_A3399 0.73 +0.1 3,915,798 - -0.9 3,915,910 + -0.9 3,915,910 + +0.2 3,915,911 - +0.1
Or see this region's nucleotide sequence