Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19575 and BWI76_RS19580 are separated by 8 nucleotides BWI76_RS19580 and BWI76_RS19585 are separated by 24 nucleotides BWI76_RS19585 and BWI76_RS19590 are separated by 11 nucleotides
BWI76_RS19575: BWI76_RS19575 - maleylacetoacetate isomerase, at 4,092,995 to 4,093,639
_RS19575
BWI76_RS19580: BWI76_RS19580 - FAA-hydrolase, at 4,093,648 to 4,094,346
_RS19580
BWI76_RS19585: BWI76_RS19585 - gentisate 1,2-dioxygenase, at 4,094,371 to 4,095,408
_RS19585
BWI76_RS19590: BWI76_RS19590 - MFS transporter, at 4,095,420 to 4,096,778
_RS19590
Position (kb)
4094
4095
4096 Strain fitness (log2 ratio)
-1
0
1 at 4093.886 kb on - strand, within BWI76_RS19580 at 4093.996 kb on + strand, within BWI76_RS19580 at 4094.384 kb on + strand at 4094.384 kb on + strand at 4094.553 kb on - strand, within BWI76_RS19585 at 4094.733 kb on - strand, within BWI76_RS19585 at 4094.905 kb on - strand, within BWI76_RS19585 at 4095.479 kb on + strand at 4095.500 kb on + strand at 4095.641 kb on - strand, within BWI76_RS19590 at 4095.812 kb on - strand, within BWI76_RS19590 at 4096.395 kb on - strand, within BWI76_RS19590
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 4,093,886 - BWI76_RS19580 0.34 -1.0 4,093,996 + BWI76_RS19580 0.50 +0.0 4,094,384 + +0.1 4,094,384 + -1.5 4,094,553 - BWI76_RS19585 0.18 -0.8 4,094,733 - BWI76_RS19585 0.35 -0.9 4,094,905 - BWI76_RS19585 0.51 -1.1 4,095,479 + -0.2 4,095,500 + +0.6 4,095,641 - BWI76_RS19590 0.16 +0.1 4,095,812 - BWI76_RS19590 0.29 +0.2 4,096,395 - BWI76_RS19590 0.72 +0.1
Or see this region's nucleotide sequence