Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10990 and BWI76_RS10995 are separated by 122 nucleotides BWI76_RS10995 and BWI76_RS11000 are separated by 135 nucleotides BWI76_RS11000 and BWI76_RS11005 are separated by 65 nucleotides
BWI76_RS10990: BWI76_RS10990 - dihydroorotase, at 2,318,330 to 2,319,376
_RS10990
BWI76_RS10995: BWI76_RS10995 - lipoprotein, at 2,319,499 to 2,320,059
_RS10995
BWI76_RS11000: BWI76_RS11000 - glutaredoxin 2, at 2,320,195 to 2,320,842
_RS11000
BWI76_RS11005: BWI76_RS11005 - MFS transporter, at 2,320,908 to 2,322,116
_RS11005
Position (kb)
2319
2320
2321 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2318.567 kb on + strand at 2318.866 kb on - strand, within BWI76_RS10990 at 2319.099 kb on - strand, within BWI76_RS10990 at 2319.372 kb on + strand at 2319.431 kb on + strand at 2319.761 kb on + strand, within BWI76_RS10995 at 2319.769 kb on - strand, within BWI76_RS10995 at 2319.838 kb on - strand, within BWI76_RS10995 at 2319.947 kb on - strand, within BWI76_RS10995 at 2319.989 kb on + strand, within BWI76_RS10995 at 2320.007 kb on + strand at 2320.007 kb on - strand at 2320.020 kb on - strand at 2320.176 kb on + strand at 2320.363 kb on - strand at 2320.363 kb on - strand, within BWI76_RS11000 at 2320.742 kb on + strand, within BWI76_RS11000 at 2320.744 kb on + strand, within BWI76_RS11000 at 2320.761 kb on + strand, within BWI76_RS11000 at 2320.808 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 2,318,567 + -0.5 2,318,866 - BWI76_RS10990 0.51 -4.4 2,319,099 - BWI76_RS10990 0.73 -0.8 2,319,372 + -5.4 2,319,431 + -2.8 2,319,761 + BWI76_RS10995 0.47 +0.5 2,319,769 - BWI76_RS10995 0.48 +1.0 2,319,838 - BWI76_RS10995 0.60 +0.1 2,319,947 - BWI76_RS10995 0.80 +0.8 2,319,989 + BWI76_RS10995 0.87 +0.1 2,320,007 + +0.0 2,320,007 - -0.5 2,320,020 - -0.3 2,320,176 + +0.1 2,320,363 - +1.0 2,320,363 - BWI76_RS11000 0.26 -1.0 2,320,742 + BWI76_RS11000 0.84 +0.3 2,320,744 + BWI76_RS11000 0.85 +0.2 2,320,761 + BWI76_RS11000 0.87 +0.2 2,320,808 - +0.6
Or see this region's nucleotide sequence